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Femoral Bone Marrow Insulin Sensitivity Is Increased by Resistance Training in Elderly Female Offspring of Overweight and Obese Mothers

View Article: PubMed Central - PubMed

ABSTRACT

Bone marrow insulin sensitivity may be an important factor for bone health in addition to bone mineral density especially in insulin resistant conditions. First we aimed to study if prenatal maternal obesity plays a role in determining bone marrow insulin sensitivity in elderly female offspring. Secondly we studied if a four-month individualized resistance training intervention increases bone marrow insulin sensitivity in elderly female offspring and whether this possible positive outcome is regulated by the offspring’s mother’s obesity status. 37 frail elderly females (mean age 71.9 ± 3.1 years) of which 20 were offspring of lean/normal-weight mothers (OLM, maternal BMI ≤ 26.3 kg/m2) and 17 were offspring of obese/overweight mothers (OOM, maternal BMI ≥ 28.1 kg/m2) were studied before and after a four-month individualized resistance training intervention. Nine age- and sex-matched non-frail controls (maternal BMI ≤ 26.3 kg/m2) were studied at baseline. Femoral bone marrow (FBM) and vertebral bone marrow (VBM) insulin sensitivity were measured using [18F]fluoro-2-deoxy-D-glucose positron emission tomography with computer tomography under hyperinsulinemic euglycemic clamp. We found that bone marrow insulin sensitivity was not related to maternal obesity status but FBM insulin sensitivity correlated with whole body insulin sensitivity (R = 0.487, p = 0.001). A four-month resistance training intervention increased FBM insulin sensitivity by 47% (p = 0.006) only in OOM, while VBM insulin sensitivity remained unchanged regardless of the maternal obesity status. In conclusion, FBM and VBM glucose metabolism reacts differently to a four-month resistance training intervention in elderly women according to their maternal obesity status.

Trial registration: ClinicalTrials.gov NCT01931540

No MeSH data available.


Cylinder-shaped volumes of interests of vertebral (upper row) and femoral bone marrow (lower row) in transaxial and coronal PET/CT-images.
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pone.0163723.g002: Cylinder-shaped volumes of interests of vertebral (upper row) and femoral bone marrow (lower row) in transaxial and coronal PET/CT-images.

Mentions: All data was corrected for time decay, dead time and measured photon attenuation. Data was reconstructed with GE iterative VuePoint method and with an axial slice thickness of 3.75 mm. Volumes of interest (VOIs) were drawn on the diaphysis of the right femoral bone marrow and on the lumbar bone marrow (L1-L2) excluding the cortex (Fig 2), on the psoas major muscle and on subcutaneous adipose tissue of the hip using Carimas 2.7 software (Turku PET Centre). CT-images were used as an anatomical reference. Time activity curves were obtained as described in our previous article [6]. The transfer rate of [18F]FDG from plasma into tissue was calculated from the time-activity curves in plasma and using a Gjedde-Patlak plot as a graphical analysis [30]. Tissue glucose uptake was obtained after multiplying the transfer rate with mean plasma glucose concentration divided by lumped constant, which was set to 1.0 for bone marrow, 1.2 for skeletal muscle [31] and 1.14 for subcutaneous adipose tissue [18]. Maternal obesity status of the subjects was not known by the investigators during the PET/CT-image processing and analysis.


Femoral Bone Marrow Insulin Sensitivity Is Increased by Resistance Training in Elderly Female Offspring of Overweight and Obese Mothers
Cylinder-shaped volumes of interests of vertebral (upper row) and femoral bone marrow (lower row) in transaxial and coronal PET/CT-images.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036877&req=5

pone.0163723.g002: Cylinder-shaped volumes of interests of vertebral (upper row) and femoral bone marrow (lower row) in transaxial and coronal PET/CT-images.
Mentions: All data was corrected for time decay, dead time and measured photon attenuation. Data was reconstructed with GE iterative VuePoint method and with an axial slice thickness of 3.75 mm. Volumes of interest (VOIs) were drawn on the diaphysis of the right femoral bone marrow and on the lumbar bone marrow (L1-L2) excluding the cortex (Fig 2), on the psoas major muscle and on subcutaneous adipose tissue of the hip using Carimas 2.7 software (Turku PET Centre). CT-images were used as an anatomical reference. Time activity curves were obtained as described in our previous article [6]. The transfer rate of [18F]FDG from plasma into tissue was calculated from the time-activity curves in plasma and using a Gjedde-Patlak plot as a graphical analysis [30]. Tissue glucose uptake was obtained after multiplying the transfer rate with mean plasma glucose concentration divided by lumped constant, which was set to 1.0 for bone marrow, 1.2 for skeletal muscle [31] and 1.14 for subcutaneous adipose tissue [18]. Maternal obesity status of the subjects was not known by the investigators during the PET/CT-image processing and analysis.

View Article: PubMed Central - PubMed

ABSTRACT

Bone marrow insulin sensitivity may be an important factor for bone health in addition to bone mineral density especially in insulin resistant conditions. First we aimed to study if prenatal maternal obesity plays a role in determining bone marrow insulin sensitivity in elderly female offspring. Secondly we studied if a four-month individualized resistance training intervention increases bone marrow insulin sensitivity in elderly female offspring and whether this possible positive outcome is regulated by the offspring’s mother’s obesity status. 37 frail elderly females (mean age 71.9 ± 3.1 years) of which 20 were offspring of lean/normal-weight mothers (OLM, maternal BMI ≤ 26.3 kg/m2) and 17 were offspring of obese/overweight mothers (OOM, maternal BMI ≥ 28.1 kg/m2) were studied before and after a four-month individualized resistance training intervention. Nine age- and sex-matched non-frail controls (maternal BMI ≤ 26.3 kg/m2) were studied at baseline. Femoral bone marrow (FBM) and vertebral bone marrow (VBM) insulin sensitivity were measured using [18F]fluoro-2-deoxy-D-glucose positron emission tomography with computer tomography under hyperinsulinemic euglycemic clamp. We found that bone marrow insulin sensitivity was not related to maternal obesity status but FBM insulin sensitivity correlated with whole body insulin sensitivity (R = 0.487, p = 0.001). A four-month resistance training intervention increased FBM insulin sensitivity by 47% (p = 0.006) only in OOM, while VBM insulin sensitivity remained unchanged regardless of the maternal obesity status. In conclusion, FBM and VBM glucose metabolism reacts differently to a four-month resistance training intervention in elderly women according to their maternal obesity status.

Trial registration: ClinicalTrials.gov NCT01931540

No MeSH data available.