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Suppression of Very Early Stage Of Adipogenesis by Baicalein, a Plant-Derived Flavonoid through Reduced Akt-C/EBP α -GLUT4 Signaling-Mediated Glucose Uptake in 3T3-L1 Adipocytes

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ABSTRACT

Baicalein has been used as a Chinese medicine, and is an abundant plant flavonoid present in fruits and vegetables. Here, we examined the effects of baicalein in adipogenesis and investigated its molecular mechanism in adipocytes. Baicalein lowered the intracellular lipid accumulation and decreased the transcription levels of the adipocyte-specific genes in mouse 3T3-L1 adipocytes. Glucose uptake mediated by glucose transporter 4 (GLUT4) was reduced, causing down-regulation of the intracellular lipid accumulation. These reductions were also observed even when baicalein was added in only early stage of adipogenesis (0–2 days) of 6-day-adipogenesis. Chromatin immunoprecipitation assay showed that baicalein decreased the binding level of C/EBPα protein to the promoter region of the GLUT4 gene. Phosphorylation of Akt at 1 h after the initiation of adipogenesis was inhibited by the treatment with baicalein. Inhibition during only the first 1.5 h after the initiation of adipogenesis by baicalein or an Akt inhibitor was enough to decrease the lipid contents in the cells undergoing adipocyte differentiation for 6 days. These results indicate that baicalein decreased the intracellular lipid accumulation by down-regulation of glucose uptake via repression of Akt-C/EBPα-GLUT4 signaling in the very early stage of adipogenesis of 3T3-L1 adipocytes.

No MeSH data available.


Change of expression of proteins associated with adipogenesis in baicalein-treated 3T3-L1 cells.3T3-L1 cells were differentiated as described in Fig 2A. Protein levels were detected by Western blot analysis using cell extracts (15 μg/lane).
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pone.0163640.g003: Change of expression of proteins associated with adipogenesis in baicalein-treated 3T3-L1 cells.3T3-L1 cells were differentiated as described in Fig 2A. Protein levels were detected by Western blot analysis using cell extracts (15 μg/lane).

Mentions: Moreover, we investigated the protein expression in Western blot analysis. The expression of PPARγ, C/EBPα, FAS, SCD, and GLUT4 was enhanced during adipogenesis, and their levels were lowered by the treatment with baicalein (Fig 3).


Suppression of Very Early Stage Of Adipogenesis by Baicalein, a Plant-Derived Flavonoid through Reduced Akt-C/EBP α -GLUT4 Signaling-Mediated Glucose Uptake in 3T3-L1 Adipocytes
Change of expression of proteins associated with adipogenesis in baicalein-treated 3T3-L1 cells.3T3-L1 cells were differentiated as described in Fig 2A. Protein levels were detected by Western blot analysis using cell extracts (15 μg/lane).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC5036867&req=5

pone.0163640.g003: Change of expression of proteins associated with adipogenesis in baicalein-treated 3T3-L1 cells.3T3-L1 cells were differentiated as described in Fig 2A. Protein levels were detected by Western blot analysis using cell extracts (15 μg/lane).
Mentions: Moreover, we investigated the protein expression in Western blot analysis. The expression of PPARγ, C/EBPα, FAS, SCD, and GLUT4 was enhanced during adipogenesis, and their levels were lowered by the treatment with baicalein (Fig 3).

View Article: PubMed Central - PubMed

ABSTRACT

Baicalein has been used as a Chinese medicine, and is an abundant plant flavonoid present in fruits and vegetables. Here, we examined the effects of baicalein in adipogenesis and investigated its molecular mechanism in adipocytes. Baicalein lowered the intracellular lipid accumulation and decreased the transcription levels of the adipocyte-specific genes in mouse 3T3-L1 adipocytes. Glucose uptake mediated by glucose transporter 4 (GLUT4) was reduced, causing down-regulation of the intracellular lipid accumulation. These reductions were also observed even when baicalein was added in only early stage of adipogenesis (0–2 days) of 6-day-adipogenesis. Chromatin immunoprecipitation assay showed that baicalein decreased the binding level of C/EBPα protein to the promoter region of the GLUT4 gene. Phosphorylation of Akt at 1 h after the initiation of adipogenesis was inhibited by the treatment with baicalein. Inhibition during only the first 1.5 h after the initiation of adipogenesis by baicalein or an Akt inhibitor was enough to decrease the lipid contents in the cells undergoing adipocyte differentiation for 6 days. These results indicate that baicalein decreased the intracellular lipid accumulation by down-regulation of glucose uptake via repression of Akt-C/EBPα-GLUT4 signaling in the very early stage of adipogenesis of 3T3-L1 adipocytes.

No MeSH data available.