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Identification of Genes Potentially Associated with the Fertility Instability of S-Type Cytoplasmic Male Sterility in Maize via Bulked Segregant RNA-Seq

View Article: PubMed Central - PubMed

ABSTRACT

S-type cytoplasmic male sterility (CMS-S) is the largest group among the three major types of CMS in maize. CMS-S exhibits fertility instability as a partial fertility restoration in a specific nuclear genetic background, which impedes its commercial application in hybrid breeding programs. The fertility instability phenomenon of CMS-S is controlled by several minor quantitative trait locus (QTLs), but not the major nuclear fertility restorer (Rf3). However, the gene mapping of these minor QTLs and the molecular mechanism of the genetic modifications are still unclear. Using completely sterile and partially rescued plants of fertility instable line (FIL)-B, we performed bulk segregant RNA-Seq and identified six potential associated genes in minor effect QTLs contributing to fertility instability. Analyses demonstrate that these potential associated genes may be involved in biological processes, such as floral organ differentiation and development regulation, energy metabolism and carbohydrates biosynthesis, which results in a partial anther exsertion and pollen fertility restoration in the partially rescued plants. The single nucleotide polymorphisms (SNPs) identified in two potential associated genes were validated to be related to the fertility restoration phenotype by KASP marker assays. This novel knowledge contributes to the understanding of the molecular mechanism of the partial fertility restoration of CMS-S in maize and thus helps to guide the breeding programs.

No MeSH data available.


Related in: MedlinePlus

Pollen staining with I2-KI revealed the normal development of a small portion of pollen from the partially rescued FIL-B.Representative images of the stained pollen of Jing724 (A) and the partially rescued (B) and sterile (C) individuals of FIL-B. Pollen was collected when the anthers exserted in the partially rescued plants. Round pollen with black staining was recorded as normal. The scale bars represent 200 μm.
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pone.0163489.g002: Pollen staining with I2-KI revealed the normal development of a small portion of pollen from the partially rescued FIL-B.Representative images of the stained pollen of Jing724 (A) and the partially rescued (B) and sterile (C) individuals of FIL-B. Pollen was collected when the anthers exserted in the partially rescued plants. Round pollen with black staining was recorded as normal. The scale bars represent 200 μm.

Mentions: Although a small portion of pollen from the partially rescued plants showed normal development with black staining (Fig 2), the majority exhibited an irregular shape without staining, which was similar to the pollen from the sterile plants. No pollen was stained in the sterile plants, but 5.56% of the pollen from the partially rescued plants was stained to show normal starch accumulation, indicating that pollen of FIL-B is fertility instable with possible fertility restoration.


Identification of Genes Potentially Associated with the Fertility Instability of S-Type Cytoplasmic Male Sterility in Maize via Bulked Segregant RNA-Seq
Pollen staining with I2-KI revealed the normal development of a small portion of pollen from the partially rescued FIL-B.Representative images of the stained pollen of Jing724 (A) and the partially rescued (B) and sterile (C) individuals of FIL-B. Pollen was collected when the anthers exserted in the partially rescued plants. Round pollen with black staining was recorded as normal. The scale bars represent 200 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036866&req=5

pone.0163489.g002: Pollen staining with I2-KI revealed the normal development of a small portion of pollen from the partially rescued FIL-B.Representative images of the stained pollen of Jing724 (A) and the partially rescued (B) and sterile (C) individuals of FIL-B. Pollen was collected when the anthers exserted in the partially rescued plants. Round pollen with black staining was recorded as normal. The scale bars represent 200 μm.
Mentions: Although a small portion of pollen from the partially rescued plants showed normal development with black staining (Fig 2), the majority exhibited an irregular shape without staining, which was similar to the pollen from the sterile plants. No pollen was stained in the sterile plants, but 5.56% of the pollen from the partially rescued plants was stained to show normal starch accumulation, indicating that pollen of FIL-B is fertility instable with possible fertility restoration.

View Article: PubMed Central - PubMed

ABSTRACT

S-type cytoplasmic male sterility (CMS-S) is the largest group among the three major types of CMS in maize. CMS-S exhibits fertility instability as a partial fertility restoration in a specific nuclear genetic background, which impedes its commercial application in hybrid breeding programs. The fertility instability phenomenon of CMS-S is controlled by several minor quantitative trait locus (QTLs), but not the major nuclear fertility restorer (Rf3). However, the gene mapping of these minor QTLs and the molecular mechanism of the genetic modifications are still unclear. Using completely sterile and partially rescued plants of fertility instable line (FIL)-B, we performed bulk segregant RNA-Seq and identified six potential associated genes in minor effect QTLs contributing to fertility instability. Analyses demonstrate that these potential associated genes may be involved in biological processes, such as floral organ differentiation and development regulation, energy metabolism and carbohydrates biosynthesis, which results in a partial anther exsertion and pollen fertility restoration in the partially rescued plants. The single nucleotide polymorphisms (SNPs) identified in two potential associated genes were validated to be related to the fertility restoration phenotype by KASP marker assays. This novel knowledge contributes to the understanding of the molecular mechanism of the partial fertility restoration of CMS-S in maize and thus helps to guide the breeding programs.

No MeSH data available.


Related in: MedlinePlus