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Identification of Genes Potentially Associated with the Fertility Instability of S-Type Cytoplasmic Male Sterility in Maize via Bulked Segregant RNA-Seq

View Article: PubMed Central - PubMed

ABSTRACT

S-type cytoplasmic male sterility (CMS-S) is the largest group among the three major types of CMS in maize. CMS-S exhibits fertility instability as a partial fertility restoration in a specific nuclear genetic background, which impedes its commercial application in hybrid breeding programs. The fertility instability phenomenon of CMS-S is controlled by several minor quantitative trait locus (QTLs), but not the major nuclear fertility restorer (Rf3). However, the gene mapping of these minor QTLs and the molecular mechanism of the genetic modifications are still unclear. Using completely sterile and partially rescued plants of fertility instable line (FIL)-B, we performed bulk segregant RNA-Seq and identified six potential associated genes in minor effect QTLs contributing to fertility instability. Analyses demonstrate that these potential associated genes may be involved in biological processes, such as floral organ differentiation and development regulation, energy metabolism and carbohydrates biosynthesis, which results in a partial anther exsertion and pollen fertility restoration in the partially rescued plants. The single nucleotide polymorphisms (SNPs) identified in two potential associated genes were validated to be related to the fertility restoration phenotype by KASP marker assays. This novel knowledge contributes to the understanding of the molecular mechanism of the partial fertility restoration of CMS-S in maize and thus helps to guide the breeding programs.

No MeSH data available.


Related in: MedlinePlus

Partially restored anther exsertion in the FIL-B population.A. Representative image of the tassels of Jing724 and FIL-B individuals with partial anther exsertion and complete sterility at six days after tasseling. The scale bars represent 3 cm. B. Representative image of the anthers harvested from A. The scale bars represent 3 cm.
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pone.0163489.g001: Partially restored anther exsertion in the FIL-B population.A. Representative image of the tassels of Jing724 and FIL-B individuals with partial anther exsertion and complete sterility at six days after tasseling. The scale bars represent 3 cm. B. Representative image of the anthers harvested from A. The scale bars represent 3 cm.

Mentions: Anther exsertion was first observed in FIL-B population 3 days after tasseling, and 3 additional days later, approximately 1/3 of the plants showed anther exsertion (Fig 1A), which categorized the FIL-B as grade II fertility according to the classification in Feng et al.[17]. Unlike Jing724, anthers of FIL-B showed no anther dehiscence or pollen shedding (Fig 1B). The anther size of the partially rescued FIL-B was smaller than the Jing724 but was comparable to the sterile anthers. The anthers of the partially rescued plants exhibited an intermediate color and shape when compared to the Jing724 and sterile individuals. Anther exsertion served as the phenotypic classification of fertility in the BC4 population, which characterized 14.9% of the 462 plants as partially rescued. These data suggest that the fertility instability of FIL-B is regulated by multiple loci.


Identification of Genes Potentially Associated with the Fertility Instability of S-Type Cytoplasmic Male Sterility in Maize via Bulked Segregant RNA-Seq
Partially restored anther exsertion in the FIL-B population.A. Representative image of the tassels of Jing724 and FIL-B individuals with partial anther exsertion and complete sterility at six days after tasseling. The scale bars represent 3 cm. B. Representative image of the anthers harvested from A. The scale bars represent 3 cm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036866&req=5

pone.0163489.g001: Partially restored anther exsertion in the FIL-B population.A. Representative image of the tassels of Jing724 and FIL-B individuals with partial anther exsertion and complete sterility at six days after tasseling. The scale bars represent 3 cm. B. Representative image of the anthers harvested from A. The scale bars represent 3 cm.
Mentions: Anther exsertion was first observed in FIL-B population 3 days after tasseling, and 3 additional days later, approximately 1/3 of the plants showed anther exsertion (Fig 1A), which categorized the FIL-B as grade II fertility according to the classification in Feng et al.[17]. Unlike Jing724, anthers of FIL-B showed no anther dehiscence or pollen shedding (Fig 1B). The anther size of the partially rescued FIL-B was smaller than the Jing724 but was comparable to the sterile anthers. The anthers of the partially rescued plants exhibited an intermediate color and shape when compared to the Jing724 and sterile individuals. Anther exsertion served as the phenotypic classification of fertility in the BC4 population, which characterized 14.9% of the 462 plants as partially rescued. These data suggest that the fertility instability of FIL-B is regulated by multiple loci.

View Article: PubMed Central - PubMed

ABSTRACT

S-type cytoplasmic male sterility (CMS-S) is the largest group among the three major types of CMS in maize. CMS-S exhibits fertility instability as a partial fertility restoration in a specific nuclear genetic background, which impedes its commercial application in hybrid breeding programs. The fertility instability phenomenon of CMS-S is controlled by several minor quantitative trait locus (QTLs), but not the major nuclear fertility restorer (Rf3). However, the gene mapping of these minor QTLs and the molecular mechanism of the genetic modifications are still unclear. Using completely sterile and partially rescued plants of fertility instable line (FIL)-B, we performed bulk segregant RNA-Seq and identified six potential associated genes in minor effect QTLs contributing to fertility instability. Analyses demonstrate that these potential associated genes may be involved in biological processes, such as floral organ differentiation and development regulation, energy metabolism and carbohydrates biosynthesis, which results in a partial anther exsertion and pollen fertility restoration in the partially rescued plants. The single nucleotide polymorphisms (SNPs) identified in two potential associated genes were validated to be related to the fertility restoration phenotype by KASP marker assays. This novel knowledge contributes to the understanding of the molecular mechanism of the partial fertility restoration of CMS-S in maize and thus helps to guide the breeding programs.

No MeSH data available.


Related in: MedlinePlus