Limits...
N 6 -(2-Hydroxyethyl)-Adenosine Exhibits Insecticidal Activity against Plutella xylostella via Adenosine Receptors

View Article: PubMed Central - PubMed

ABSTRACT

The diamondback moth, Plutella xylostella, is one of the most important pests of cruciferous crops. We have earlier shown that N6-(2-hydroxyethyl)-adenosine (HEA) exhibits insecticidal activity against P. xylostella. In the present study we investigated the possible mechanism of insecticidal action of HEA on P. xylostella. HEA is a derivative of adenosine, therefore, we speculated whether it acts via P. xylostella adenosine receptor (PxAdoR). We used RNAi approach to silence PxAdoR gene and used antagonist of denosine receptor (AdoR) to study the insecticidal effect of HEA. We cloned the whole sequence of PxAdoR gene. A BLAST search using NCBI protein database showed a 61% identity with the Drosophila adenosine receptor (DmAdoR) and a 32–35% identity with human AdoR. Though the amino acids sequence of PxAdoR was different compared to other adenosine receptors, most of the amino acids that are known to be important for adenosine receptor ligand binding and signaling were present. However, only 30% binding sites key residues was similar between PxAdoR and A1R. HEA, at a dose of 1 mg/mL, was found to be lethal to the second-instar larvae of P. xylostella, and a significant reduction of mortality and growth inhibition ratio were obtained when HEA was administered to the larvae along with PxAdoR-dsRNA or antagonist of AdoR (SCH58261) for 36, 48, or 60 h. Especially at 48 h, the rate of growth inhibition of the PxAdoR knockdown group was 3.5-fold less than that of the HEA group, and the corrected mortality of SCH58261 group was reduced almost 2-fold compared with the HEA group. Our findings show that HEA may exert its insecticidal activity against P. xylostella larvae via acting on PxAdoR.

No MeSH data available.


Alignment of the human A2b adenosine receptor and N-terminal part of AdoR amino acids sequences of other insects and Plutella xylostella.Different color shows different homology levels. Black means 100% homology level, pink shows 75% homology level, blue stands for 50% homology level, and yellow marks 33% homology level. The PxAdoR and human adenosine receptors share the same key residues marked by red solid border and the mismatched key residues between PxAdoR and human adenosine receptors are marked by black solid border. “#” sign indicates the special residues of PxAdoR compared with human adenosine receptors.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5036850&req=5

pone.0162859.g002: Alignment of the human A2b adenosine receptor and N-terminal part of AdoR amino acids sequences of other insects and Plutella xylostella.Different color shows different homology levels. Black means 100% homology level, pink shows 75% homology level, blue stands for 50% homology level, and yellow marks 33% homology level. The PxAdoR and human adenosine receptors share the same key residues marked by red solid border and the mismatched key residues between PxAdoR and human adenosine receptors are marked by black solid border. “#” sign indicates the special residues of PxAdoR compared with human adenosine receptors.

Mentions: The predicted ORF of the PxAdoR gene encodes a protein of 440 amino acids. Using the method of Clustal W, a comparison of amino acids sequence showed that the N-terminal sequence of PxAdoR had a low homology with human A2b adenosine receptor, but was more similar with Drosophila AdoR and other insects AdoRs. The human adenosine receptor have 193 binding sites key residues [18], only 71 binding sites key residues of A1R, 84 of A2aR, 85 of A2bR, and 64 of A3R are identical compared to that of PxAdoR (Fig 2).


N 6 -(2-Hydroxyethyl)-Adenosine Exhibits Insecticidal Activity against Plutella xylostella via Adenosine Receptors
Alignment of the human A2b adenosine receptor and N-terminal part of AdoR amino acids sequences of other insects and Plutella xylostella.Different color shows different homology levels. Black means 100% homology level, pink shows 75% homology level, blue stands for 50% homology level, and yellow marks 33% homology level. The PxAdoR and human adenosine receptors share the same key residues marked by red solid border and the mismatched key residues between PxAdoR and human adenosine receptors are marked by black solid border. “#” sign indicates the special residues of PxAdoR compared with human adenosine receptors.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036850&req=5

pone.0162859.g002: Alignment of the human A2b adenosine receptor and N-terminal part of AdoR amino acids sequences of other insects and Plutella xylostella.Different color shows different homology levels. Black means 100% homology level, pink shows 75% homology level, blue stands for 50% homology level, and yellow marks 33% homology level. The PxAdoR and human adenosine receptors share the same key residues marked by red solid border and the mismatched key residues between PxAdoR and human adenosine receptors are marked by black solid border. “#” sign indicates the special residues of PxAdoR compared with human adenosine receptors.
Mentions: The predicted ORF of the PxAdoR gene encodes a protein of 440 amino acids. Using the method of Clustal W, a comparison of amino acids sequence showed that the N-terminal sequence of PxAdoR had a low homology with human A2b adenosine receptor, but was more similar with Drosophila AdoR and other insects AdoRs. The human adenosine receptor have 193 binding sites key residues [18], only 71 binding sites key residues of A1R, 84 of A2aR, 85 of A2bR, and 64 of A3R are identical compared to that of PxAdoR (Fig 2).

View Article: PubMed Central - PubMed

ABSTRACT

The diamondback moth, Plutella xylostella, is one of the most important pests of cruciferous crops. We have earlier shown that N6-(2-hydroxyethyl)-adenosine (HEA) exhibits insecticidal activity against P. xylostella. In the present study we investigated the possible mechanism of insecticidal action of HEA on P. xylostella. HEA is a derivative of adenosine, therefore, we speculated whether it acts via P. xylostella adenosine receptor (PxAdoR). We used RNAi approach to silence PxAdoR gene and used antagonist of denosine receptor (AdoR) to study the insecticidal effect of HEA. We cloned the whole sequence of PxAdoR gene. A BLAST search using NCBI protein database showed a 61% identity with the Drosophila adenosine receptor (DmAdoR) and a 32–35% identity with human AdoR. Though the amino acids sequence of PxAdoR was different compared to other adenosine receptors, most of the amino acids that are known to be important for adenosine receptor ligand binding and signaling were present. However, only 30% binding sites key residues was similar between PxAdoR and A1R. HEA, at a dose of 1 mg/mL, was found to be lethal to the second-instar larvae of P. xylostella, and a significant reduction of mortality and growth inhibition ratio were obtained when HEA was administered to the larvae along with PxAdoR-dsRNA or antagonist of AdoR (SCH58261) for 36, 48, or 60 h. Especially at 48 h, the rate of growth inhibition of the PxAdoR knockdown group was 3.5-fold less than that of the HEA group, and the corrected mortality of SCH58261 group was reduced almost 2-fold compared with the HEA group. Our findings show that HEA may exert its insecticidal activity against P. xylostella larvae via acting on PxAdoR.

No MeSH data available.