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Analysis of SOX2-Regulated Transcriptome in Glioma Stem Cells

View Article: PubMed Central - PubMed

ABSTRACT

Introduction: Glioblastoma is the most malignant brain tumor in adults and is associated with poor survival despite multimodal treatments. Glioma stem-like cells (GSCs) are cells functionally defined by their self-renewal potential and the ability to reconstitute the original tumor upon orthotopic implantation. They have been postulated to be the culprit of glioma chemo- and radio-resistance ultimately leading to relapse. Understanding the molecular circuits governing the GSC compartment is essential. SOX2, a critical transcription regulator of embryonic and neural stem cell function, is deregulated in GSCs however; the precise molecular pathways regulated by this gene in GSCs remain poorly understood.

Results: We performed a genome-wide analysis of SOX2-regulated transcripts in GSCs, using a microarray. We identified a total of 2048 differentially expressed coding transcripts and 261 non-coding transcripts. Cell adhesion and cell-cell signaling are among the most enriched terms using Gene Ontology (GO) classification. The pathways altered after SOX2 down-modulation includes multiple cellular processes such as amino-acid metabolism and intercellular signaling cascades. We also defined and classified the set of non-coding transcripts differentially expressed regulated by SOX2 in GSCs, and validated two of them.

Conclusions: We present a comprehensive analysis of the transcriptome controlled by SOX2 in GSCs, gaining insights in the understanding of the potential roles of SOX2 in glioblastoma.

No MeSH data available.


Related in: MedlinePlus

Top-10 GO Biological Processes analysis of protein-coding genes regulated by SOX2 in GSC11 cells.Bar chart represents classification of GO Biological Processes, Cellular Component or Molecular Function as determined by DAVID web tool. Bars represent the number of genes in the specified category, organized by p-value.
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pone.0163155.g003: Top-10 GO Biological Processes analysis of protein-coding genes regulated by SOX2 in GSC11 cells.Bar chart represents classification of GO Biological Processes, Cellular Component or Molecular Function as determined by DAVID web tool. Bars represent the number of genes in the specified category, organized by p-value.

Mentions: Enrichment analysis of GO categories including biological process (BP), molecular function (MF), and cellular component (CC) were obtained using DAVID web tool (Fig 3). We observed the highest enrichment in the categories related to “cell adhesion”, “biological adhesion”, “cell-cell signaling”, “extracellular region” and “calcium ion binding”.


Analysis of SOX2-Regulated Transcriptome in Glioma Stem Cells
Top-10 GO Biological Processes analysis of protein-coding genes regulated by SOX2 in GSC11 cells.Bar chart represents classification of GO Biological Processes, Cellular Component or Molecular Function as determined by DAVID web tool. Bars represent the number of genes in the specified category, organized by p-value.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036841&req=5

pone.0163155.g003: Top-10 GO Biological Processes analysis of protein-coding genes regulated by SOX2 in GSC11 cells.Bar chart represents classification of GO Biological Processes, Cellular Component or Molecular Function as determined by DAVID web tool. Bars represent the number of genes in the specified category, organized by p-value.
Mentions: Enrichment analysis of GO categories including biological process (BP), molecular function (MF), and cellular component (CC) were obtained using DAVID web tool (Fig 3). We observed the highest enrichment in the categories related to “cell adhesion”, “biological adhesion”, “cell-cell signaling”, “extracellular region” and “calcium ion binding”.

View Article: PubMed Central - PubMed

ABSTRACT

Introduction: Glioblastoma is the most malignant brain tumor in adults and is associated with poor survival despite multimodal treatments. Glioma stem-like cells (GSCs) are cells functionally defined by their self-renewal potential and the ability to reconstitute the original tumor upon orthotopic implantation. They have been postulated to be the culprit of glioma chemo- and radio-resistance ultimately leading to relapse. Understanding the molecular circuits governing the GSC compartment is essential. SOX2, a critical transcription regulator of embryonic and neural stem cell function, is deregulated in GSCs however; the precise molecular pathways regulated by this gene in GSCs remain poorly understood.

Results: We performed a genome-wide analysis of SOX2-regulated transcripts in GSCs, using a microarray. We identified a total of 2048 differentially expressed coding transcripts and 261 non-coding transcripts. Cell adhesion and cell-cell signaling are among the most enriched terms using Gene Ontology (GO) classification. The pathways altered after SOX2 down-modulation includes multiple cellular processes such as amino-acid metabolism and intercellular signaling cascades. We also defined and classified the set of non-coding transcripts differentially expressed regulated by SOX2 in GSCs, and validated two of them.

Conclusions: We present a comprehensive analysis of the transcriptome controlled by SOX2 in GSCs, gaining insights in the understanding of the potential roles of SOX2 in glioblastoma.

No MeSH data available.


Related in: MedlinePlus