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Characterization of the Expression of the RNA Binding Protein eIF4G1 and Its Clinicopathological Correlation with Serous Ovarian Cancer

View Article: PubMed Central - PubMed

ABSTRACT

Background: Ovarian cancer is the most lethal type of malignant tumor in gynecological cancers and is associated with a high percentage of late diagnosis and chemotherapy resistance. Thus, it is urgent to identify a tumor marker or a molecular target that allows early detection and effective treatment. RNA-binding proteins (RBPs) are crucial in various cellular processes at the post-transcriptional level. The eukaryotic translation initiation factor 4 gamma, 1(eIF4G1), an RNA-binding protein, facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. However, little is known regarding the characteristics of eIF4G1 expression and its clinical significance in ovarian cancer. Therefore, we propose to investigate the expression and clinicopathological significance of eIF4G1 in ovarian cancer patients.

Methods: We performed Real-time PCR in 40 fresh serous ovarian cancer tissues and 27 normal ovarian surface epithelial cell specimens to assess eIF4G1mRNA expression. Immunohistochemistry (IHC) was used to examine the expression of eIF4G1 at the protein level in 134 patients with serous ovarian cancer and 18 normal ovarian tissues. Statistical analysis was conducted to determine the correlation of the eIF4G1 protein levels with the clinicopathological characteristics and prognosis in ovarian cancer.

Results: The expression of eIF4G1 was upregulated in serous ovarian cancer tissues at both the mRNA (P = 0.0375) and the protein (P = 0.0007) levels. The eIF4G1 expression was significantly correlated with the clinical tumor stage (P = 0.0004) and omentum metastasis (P = 0.024). Moreover, patients with low eIF4G1 protein expression had a longer overall survival time (P = 0.026).

Conclusions: These data revealed that eIF4G1 is markedly expressed in serous ovarian cancer and that upregulation of the eIF4G1 protein expression is significantly associated with an advanced tumor stage. Besides, the patients with lower expression of eIF4G1 tend to have a longer overall survival time. Thus, eIF4G1 may contribute to the occurrence and metastasis of ovarian cancer and can serve as a potential therapeutic target for the treatment of ovarian cancer.

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The expression of eIF4G1 at the protein level in ovarian cancer tissues from patients.(Left, ×40; Right, ×100) Immunohistochemical staining of eIF4G1 in ovarian cancer tissue at different staining score using anti-human eIF4G1 antibodies. (Left magnification, ×40; Right magnification, ×100).
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pone.0163447.g002: The expression of eIF4G1 at the protein level in ovarian cancer tissues from patients.(Left, ×40; Right, ×100) Immunohistochemical staining of eIF4G1 in ovarian cancer tissue at different staining score using anti-human eIF4G1 antibodies. (Left magnification, ×40; Right magnification, ×100).

Mentions: To further determine whether eIF4G1 protein expression is consistent with the results for mRNA expression, we used three tissue microarrays, which contained the cores from 134 serous epithelial ovarian cancers and 18 normal ovaries to localize and quantify eIF4G1 expression. Positively stained eIF4G1 was primarily located in the cytoplasm of ovarian cancer cells and manifested as light brown and brown particles (Fig 2). In contrast to OSE, the expression of eIF4G1 was much higher in the ovarian cancer samples (P = 0.0007) (Fig 3). The histochemical score was consistent with the previous RT-PCR results for eIF4G1mRNA.


Characterization of the Expression of the RNA Binding Protein eIF4G1 and Its Clinicopathological Correlation with Serous Ovarian Cancer
The expression of eIF4G1 at the protein level in ovarian cancer tissues from patients.(Left, ×40; Right, ×100) Immunohistochemical staining of eIF4G1 in ovarian cancer tissue at different staining score using anti-human eIF4G1 antibodies. (Left magnification, ×40; Right magnification, ×100).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036801&req=5

pone.0163447.g002: The expression of eIF4G1 at the protein level in ovarian cancer tissues from patients.(Left, ×40; Right, ×100) Immunohistochemical staining of eIF4G1 in ovarian cancer tissue at different staining score using anti-human eIF4G1 antibodies. (Left magnification, ×40; Right magnification, ×100).
Mentions: To further determine whether eIF4G1 protein expression is consistent with the results for mRNA expression, we used three tissue microarrays, which contained the cores from 134 serous epithelial ovarian cancers and 18 normal ovaries to localize and quantify eIF4G1 expression. Positively stained eIF4G1 was primarily located in the cytoplasm of ovarian cancer cells and manifested as light brown and brown particles (Fig 2). In contrast to OSE, the expression of eIF4G1 was much higher in the ovarian cancer samples (P = 0.0007) (Fig 3). The histochemical score was consistent with the previous RT-PCR results for eIF4G1mRNA.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Ovarian cancer is the most lethal type of malignant tumor in gynecological cancers and is associated with a high percentage of late diagnosis and chemotherapy resistance. Thus, it is urgent to identify a tumor marker or a molecular target that allows early detection and effective treatment. RNA-binding proteins (RBPs) are crucial in various cellular processes at the post-transcriptional level. The eukaryotic translation initiation factor 4 gamma, 1(eIF4G1), an RNA-binding protein, facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. However, little is known regarding the characteristics of eIF4G1 expression and its clinical significance in ovarian cancer. Therefore, we propose to investigate the expression and clinicopathological significance of eIF4G1 in ovarian cancer patients.

Methods: We performed Real-time PCR in 40 fresh serous ovarian cancer tissues and 27 normal ovarian surface epithelial cell specimens to assess eIF4G1mRNA expression. Immunohistochemistry (IHC) was used to examine the expression of eIF4G1 at the protein level in 134 patients with serous ovarian cancer and 18 normal ovarian tissues. Statistical analysis was conducted to determine the correlation of the eIF4G1 protein levels with the clinicopathological characteristics and prognosis in ovarian cancer.

Results: The expression of eIF4G1 was upregulated in serous ovarian cancer tissues at both the mRNA (P = 0.0375) and the protein (P = 0.0007) levels. The eIF4G1 expression was significantly correlated with the clinical tumor stage (P = 0.0004) and omentum metastasis (P = 0.024). Moreover, patients with low eIF4G1 protein expression had a longer overall survival time (P = 0.026).

Conclusions: These data revealed that eIF4G1 is markedly expressed in serous ovarian cancer and that upregulation of the eIF4G1 protein expression is significantly associated with an advanced tumor stage. Besides, the patients with lower expression of eIF4G1 tend to have a longer overall survival time. Thus, eIF4G1 may contribute to the occurrence and metastasis of ovarian cancer and can serve as a potential therapeutic target for the treatment of ovarian cancer.

No MeSH data available.


Related in: MedlinePlus