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Two-dimensional periodic texture of actin filaments formed upon drying

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ABSTRACT

We found that a solution of actin filaments can form a periodic texture in the process of drying on a flat glass surface in the air; the periodic texture was composed of smooth meandering bundles of actin filaments. We also found that a branched salt crystal grows in the space between the meandering bundles of actin filaments. The distance between the adjacent striae (striation period) in the resulting dried two-dimensional pattern of striation decreased from about 50 to 2 μm, as the ambient temperature was increased from 4 to 40°C at 1 mg/ml actin, and showed an increasing tendency from a few to several tens μm with the increase in the initial concentration of actin filaments from 0.6 to 2.0mg/ml at room temperature. As the speed of drying is increased at a certain temperature, the striation period was also found to decrease. We propose that the formation of the two-dimensional striation pattern of bundles of actin filaments is the result of condensation of proteins due to dehydration, and suggest that the solvent flow from the center to the periphery of the sample causes the meandering of actin filaments.

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Polarizing micrographs obtained from two different focal planes (a and b) of the sample prepared according to method 2 in a cylindrical chamber and the schematic representation of the sample well and the focal planes (c). (a) Photo taken at the focus near the surface and (b) photo taken at the near-central depth of the solution. The left part of each micrograph was covered with surface structure of the sample and the right part was focused of the area beneath them as revealed by peeling away the dried surface using tweezers, where the striation pattern was observed. Scale bar, 200 μm. The schematic illustration (c) represents the location of the edge of the peeled surface structure and the focal plane taken for panels (a) and (b).
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f8-7_11: Polarizing micrographs obtained from two different focal planes (a and b) of the sample prepared according to method 2 in a cylindrical chamber and the schematic representation of the sample well and the focal planes (c). (a) Photo taken at the focus near the surface and (b) photo taken at the near-central depth of the solution. The left part of each micrograph was covered with surface structure of the sample and the right part was focused of the area beneath them as revealed by peeling away the dried surface using tweezers, where the striation pattern was observed. Scale bar, 200 μm. The schematic illustration (c) represents the location of the edge of the peeled surface structure and the focal plane taken for panels (a) and (b).

Mentions: Figure 8 shows the polarizing micrographs of the F-actin solution (10mg/ml), of which the film-like structure was partly peeled in the right area of Figure 8a and b. Figure 8a focuses on the top of the sample where the film-like structure was formed, and in Figure 8b the focus was set at about mid-depth of the sample where the striation patterns were observed. Figure 8c schematically illustrates the side view of this sample.


Two-dimensional periodic texture of actin filaments formed upon drying
Polarizing micrographs obtained from two different focal planes (a and b) of the sample prepared according to method 2 in a cylindrical chamber and the schematic representation of the sample well and the focal planes (c). (a) Photo taken at the focus near the surface and (b) photo taken at the near-central depth of the solution. The left part of each micrograph was covered with surface structure of the sample and the right part was focused of the area beneath them as revealed by peeling away the dried surface using tweezers, where the striation pattern was observed. Scale bar, 200 μm. The schematic illustration (c) represents the location of the edge of the peeled surface structure and the focal plane taken for panels (a) and (b).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5036779&req=5

f8-7_11: Polarizing micrographs obtained from two different focal planes (a and b) of the sample prepared according to method 2 in a cylindrical chamber and the schematic representation of the sample well and the focal planes (c). (a) Photo taken at the focus near the surface and (b) photo taken at the near-central depth of the solution. The left part of each micrograph was covered with surface structure of the sample and the right part was focused of the area beneath them as revealed by peeling away the dried surface using tweezers, where the striation pattern was observed. Scale bar, 200 μm. The schematic illustration (c) represents the location of the edge of the peeled surface structure and the focal plane taken for panels (a) and (b).
Mentions: Figure 8 shows the polarizing micrographs of the F-actin solution (10mg/ml), of which the film-like structure was partly peeled in the right area of Figure 8a and b. Figure 8a focuses on the top of the sample where the film-like structure was formed, and in Figure 8b the focus was set at about mid-depth of the sample where the striation patterns were observed. Figure 8c schematically illustrates the side view of this sample.

View Article: PubMed Central - PubMed

ABSTRACT

We found that a solution of actin filaments can form a periodic texture in the process of drying on a flat glass surface in the air; the periodic texture was composed of smooth meandering bundles of actin filaments. We also found that a branched salt crystal grows in the space between the meandering bundles of actin filaments. The distance between the adjacent striae (striation period) in the resulting dried two-dimensional pattern of striation decreased from about 50 to 2 μm, as the ambient temperature was increased from 4 to 40°C at 1 mg/ml actin, and showed an increasing tendency from a few to several tens μm with the increase in the initial concentration of actin filaments from 0.6 to 2.0mg/ml at room temperature. As the speed of drying is increased at a certain temperature, the striation period was also found to decrease. We propose that the formation of the two-dimensional striation pattern of bundles of actin filaments is the result of condensation of proteins due to dehydration, and suggest that the solvent flow from the center to the periphery of the sample causes the meandering of actin filaments.

No MeSH data available.


Related in: MedlinePlus