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Two-dimensional periodic texture of actin filaments formed upon drying

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ABSTRACT

We found that a solution of actin filaments can form a periodic texture in the process of drying on a flat glass surface in the air; the periodic texture was composed of smooth meandering bundles of actin filaments. We also found that a branched salt crystal grows in the space between the meandering bundles of actin filaments. The distance between the adjacent striae (striation period) in the resulting dried two-dimensional pattern of striation decreased from about 50 to 2 μm, as the ambient temperature was increased from 4 to 40°C at 1 mg/ml actin, and showed an increasing tendency from a few to several tens μm with the increase in the initial concentration of actin filaments from 0.6 to 2.0mg/ml at room temperature. As the speed of drying is increased at a certain temperature, the striation period was also found to decrease. We propose that the formation of the two-dimensional striation pattern of bundles of actin filaments is the result of condensation of proteins due to dehydration, and suggest that the solvent flow from the center to the periphery of the sample causes the meandering of actin filaments.

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Polarizing micrograph showing the wet part of sample in the process of drying. Two mg/ml of F-actin was dried under the same condition as in Figure 1. The directions of polarizer (P) and analyzer (A) are shown by crossed bipolar arrows. The edge of the wet region is indicated by an arrow. Scale bar, 200 μm.
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f6-7_11: Polarizing micrograph showing the wet part of sample in the process of drying. Two mg/ml of F-actin was dried under the same condition as in Figure 1. The directions of polarizer (P) and analyzer (A) are shown by crossed bipolar arrows. The edge of the wet region is indicated by an arrow. Scale bar, 200 μm.

Mentions: Next we observed the pattern formation at the boundary between the wet and dry regions under a polarizing microscope. Figure 6 demonstrates that the striation pattern formed in the dried portion extends to the wet region, implying that the striation pattern begins to be formed in solution before drying. It is to be noted that the region where the striation pattern is observed is limited near the boundary region (about 10 μm wide), but no type of domain structure was observed within a region away from this boundary region.


Two-dimensional periodic texture of actin filaments formed upon drying
Polarizing micrograph showing the wet part of sample in the process of drying. Two mg/ml of F-actin was dried under the same condition as in Figure 1. The directions of polarizer (P) and analyzer (A) are shown by crossed bipolar arrows. The edge of the wet region is indicated by an arrow. Scale bar, 200 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC5036779&req=5

f6-7_11: Polarizing micrograph showing the wet part of sample in the process of drying. Two mg/ml of F-actin was dried under the same condition as in Figure 1. The directions of polarizer (P) and analyzer (A) are shown by crossed bipolar arrows. The edge of the wet region is indicated by an arrow. Scale bar, 200 μm.
Mentions: Next we observed the pattern formation at the boundary between the wet and dry regions under a polarizing microscope. Figure 6 demonstrates that the striation pattern formed in the dried portion extends to the wet region, implying that the striation pattern begins to be formed in solution before drying. It is to be noted that the region where the striation pattern is observed is limited near the boundary region (about 10 μm wide), but no type of domain structure was observed within a region away from this boundary region.

View Article: PubMed Central - PubMed

ABSTRACT

We found that a solution of actin filaments can form a periodic texture in the process of drying on a flat glass surface in the air; the periodic texture was composed of smooth meandering bundles of actin filaments. We also found that a branched salt crystal grows in the space between the meandering bundles of actin filaments. The distance between the adjacent striae (striation period) in the resulting dried two-dimensional pattern of striation decreased from about 50 to 2 μm, as the ambient temperature was increased from 4 to 40°C at 1 mg/ml actin, and showed an increasing tendency from a few to several tens μm with the increase in the initial concentration of actin filaments from 0.6 to 2.0mg/ml at room temperature. As the speed of drying is increased at a certain temperature, the striation period was also found to decrease. We propose that the formation of the two-dimensional striation pattern of bundles of actin filaments is the result of condensation of proteins due to dehydration, and suggest that the solvent flow from the center to the periphery of the sample causes the meandering of actin filaments.

No MeSH data available.


Related in: MedlinePlus