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Two-dimensional periodic texture of actin filaments formed upon drying

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ABSTRACT

We found that a solution of actin filaments can form a periodic texture in the process of drying on a flat glass surface in the air; the periodic texture was composed of smooth meandering bundles of actin filaments. We also found that a branched salt crystal grows in the space between the meandering bundles of actin filaments. The distance between the adjacent striae (striation period) in the resulting dried two-dimensional pattern of striation decreased from about 50 to 2 μm, as the ambient temperature was increased from 4 to 40°C at 1 mg/ml actin, and showed an increasing tendency from a few to several tens μm with the increase in the initial concentration of actin filaments from 0.6 to 2.0mg/ml at room temperature. As the speed of drying is increased at a certain temperature, the striation period was also found to decrease. We propose that the formation of the two-dimensional striation pattern of bundles of actin filaments is the result of condensation of proteins due to dehydration, and suggest that the solvent flow from the center to the periphery of the sample causes the meandering of actin filaments.

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Relationship between striation period and F-actin concentrations. Various concentrations of F-actin solutions were dried under the same condition as in Figure 1 on the glass slide which was mounted on the temperature-controlled brass block at 25°C. The striation period increased in accordance with the increase in the concentration of F-actin. No striation was formed at the F-actin concentration lower than 0.4mg/ml.
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f5-7_11: Relationship between striation period and F-actin concentrations. Various concentrations of F-actin solutions were dried under the same condition as in Figure 1 on the glass slide which was mounted on the temperature-controlled brass block at 25°C. The striation period increased in accordance with the increase in the concentration of F-actin. No striation was formed at the F-actin concentration lower than 0.4mg/ml.

Mentions: The effects of the F-actin concentration on the striation period were examined between 0.4 and 2mg/ml under the condition of natural drying at 25°C. The result is summarized in Figure 5, in which monotonic increase in the striation period with the increase in the F-actin concentration is seen. We confirmed that no striation was formed at F-actin concentrations of lower than 0.4mg/ml at room temperature, implying that there is a critical actin concentration for the formation of periodic texture.


Two-dimensional periodic texture of actin filaments formed upon drying
Relationship between striation period and F-actin concentrations. Various concentrations of F-actin solutions were dried under the same condition as in Figure 1 on the glass slide which was mounted on the temperature-controlled brass block at 25°C. The striation period increased in accordance with the increase in the concentration of F-actin. No striation was formed at the F-actin concentration lower than 0.4mg/ml.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5036779&req=5

f5-7_11: Relationship between striation period and F-actin concentrations. Various concentrations of F-actin solutions were dried under the same condition as in Figure 1 on the glass slide which was mounted on the temperature-controlled brass block at 25°C. The striation period increased in accordance with the increase in the concentration of F-actin. No striation was formed at the F-actin concentration lower than 0.4mg/ml.
Mentions: The effects of the F-actin concentration on the striation period were examined between 0.4 and 2mg/ml under the condition of natural drying at 25°C. The result is summarized in Figure 5, in which monotonic increase in the striation period with the increase in the F-actin concentration is seen. We confirmed that no striation was formed at F-actin concentrations of lower than 0.4mg/ml at room temperature, implying that there is a critical actin concentration for the formation of periodic texture.

View Article: PubMed Central - PubMed

ABSTRACT

We found that a solution of actin filaments can form a periodic texture in the process of drying on a flat glass surface in the air; the periodic texture was composed of smooth meandering bundles of actin filaments. We also found that a branched salt crystal grows in the space between the meandering bundles of actin filaments. The distance between the adjacent striae (striation period) in the resulting dried two-dimensional pattern of striation decreased from about 50 to 2 μm, as the ambient temperature was increased from 4 to 40°C at 1 mg/ml actin, and showed an increasing tendency from a few to several tens μm with the increase in the initial concentration of actin filaments from 0.6 to 2.0mg/ml at room temperature. As the speed of drying is increased at a certain temperature, the striation period was also found to decrease. We propose that the formation of the two-dimensional striation pattern of bundles of actin filaments is the result of condensation of proteins due to dehydration, and suggest that the solvent flow from the center to the periphery of the sample causes the meandering of actin filaments.

No MeSH data available.


Related in: MedlinePlus