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Two-dimensional periodic texture of actin filaments formed upon drying

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ABSTRACT

We found that a solution of actin filaments can form a periodic texture in the process of drying on a flat glass surface in the air; the periodic texture was composed of smooth meandering bundles of actin filaments. We also found that a branched salt crystal grows in the space between the meandering bundles of actin filaments. The distance between the adjacent striae (striation period) in the resulting dried two-dimensional pattern of striation decreased from about 50 to 2 μm, as the ambient temperature was increased from 4 to 40°C at 1 mg/ml actin, and showed an increasing tendency from a few to several tens μm with the increase in the initial concentration of actin filaments from 0.6 to 2.0mg/ml at room temperature. As the speed of drying is increased at a certain temperature, the striation period was also found to decrease. We propose that the formation of the two-dimensional striation pattern of bundles of actin filaments is the result of condensation of proteins due to dehydration, and suggest that the solvent flow from the center to the periphery of the sample causes the meandering of actin filaments.

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Relationship between striation period and the temperature at which samples were dried. F-actin solution was dried under the same conditions as in Figure 1 on the glass slide which was mounted on the temperature-controlled brass block. The temperature of the block was plotted on the abscissa. Striation period (p) was inversely proportional to temperature (T) as shown by the solid curve p = 0.11 + 58.7/(T − 3.9).
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f4-7_11: Relationship between striation period and the temperature at which samples were dried. F-actin solution was dried under the same conditions as in Figure 1 on the glass slide which was mounted on the temperature-controlled brass block. The temperature of the block was plotted on the abscissa. Striation period (p) was inversely proportional to temperature (T) as shown by the solid curve p = 0.11 + 58.7/(T − 3.9).

Mentions: When the drying temperature was increased from 4 to 40°C under the condition of natural drying, the striation period largely decreased monotonically from about 50 μm (at 4°C, Fig. 3a) to 8 μm (at 10°C, Fig. 3b). The temperature dependence of the striation period is summarized in Figure 4. The relationship between the striation period (p) and the temperature (T) was expressed by p = p0 + α/(T − T0), where α, p0 and T0 are, respectively, set to be 58.7 μm°C, 0.11 μm and 3.9°C. This relationship is equivalent to that obtained for a cholesteric liquid crystal12. On lowering the temperature, the drying speed was markedly slowed, so that it became difficult to form the clear striation pattern; in practice, the drying did not occur at lower than 1°C under the drying conditions used in the present study.


Two-dimensional periodic texture of actin filaments formed upon drying
Relationship between striation period and the temperature at which samples were dried. F-actin solution was dried under the same conditions as in Figure 1 on the glass slide which was mounted on the temperature-controlled brass block. The temperature of the block was plotted on the abscissa. Striation period (p) was inversely proportional to temperature (T) as shown by the solid curve p = 0.11 + 58.7/(T − 3.9).
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getmorefigures.php?uid=PMC5036779&req=5

f4-7_11: Relationship between striation period and the temperature at which samples were dried. F-actin solution was dried under the same conditions as in Figure 1 on the glass slide which was mounted on the temperature-controlled brass block. The temperature of the block was plotted on the abscissa. Striation period (p) was inversely proportional to temperature (T) as shown by the solid curve p = 0.11 + 58.7/(T − 3.9).
Mentions: When the drying temperature was increased from 4 to 40°C under the condition of natural drying, the striation period largely decreased monotonically from about 50 μm (at 4°C, Fig. 3a) to 8 μm (at 10°C, Fig. 3b). The temperature dependence of the striation period is summarized in Figure 4. The relationship between the striation period (p) and the temperature (T) was expressed by p = p0 + α/(T − T0), where α, p0 and T0 are, respectively, set to be 58.7 μm°C, 0.11 μm and 3.9°C. This relationship is equivalent to that obtained for a cholesteric liquid crystal12. On lowering the temperature, the drying speed was markedly slowed, so that it became difficult to form the clear striation pattern; in practice, the drying did not occur at lower than 1°C under the drying conditions used in the present study.

View Article: PubMed Central - PubMed

ABSTRACT

We found that a solution of actin filaments can form a periodic texture in the process of drying on a flat glass surface in the air; the periodic texture was composed of smooth meandering bundles of actin filaments. We also found that a branched salt crystal grows in the space between the meandering bundles of actin filaments. The distance between the adjacent striae (striation period) in the resulting dried two-dimensional pattern of striation decreased from about 50 to 2 μm, as the ambient temperature was increased from 4 to 40°C at 1 mg/ml actin, and showed an increasing tendency from a few to several tens μm with the increase in the initial concentration of actin filaments from 0.6 to 2.0mg/ml at room temperature. As the speed of drying is increased at a certain temperature, the striation period was also found to decrease. We propose that the formation of the two-dimensional striation pattern of bundles of actin filaments is the result of condensation of proteins due to dehydration, and suggest that the solvent flow from the center to the periphery of the sample causes the meandering of actin filaments.

No MeSH data available.


Related in: MedlinePlus