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Two-dimensional periodic texture of actin filaments formed upon drying

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ABSTRACT

We found that a solution of actin filaments can form a periodic texture in the process of drying on a flat glass surface in the air; the periodic texture was composed of smooth meandering bundles of actin filaments. We also found that a branched salt crystal grows in the space between the meandering bundles of actin filaments. The distance between the adjacent striae (striation period) in the resulting dried two-dimensional pattern of striation decreased from about 50 to 2 μm, as the ambient temperature was increased from 4 to 40°C at 1 mg/ml actin, and showed an increasing tendency from a few to several tens μm with the increase in the initial concentration of actin filaments from 0.6 to 2.0mg/ml at room temperature. As the speed of drying is increased at a certain temperature, the striation period was also found to decrease. We propose that the formation of the two-dimensional striation pattern of bundles of actin filaments is the result of condensation of proteins due to dehydration, and suggest that the solvent flow from the center to the periphery of the sample causes the meandering of actin filaments.

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General view of the two-dimensional texture obtained by natural drying of F-actin solution. (a) About 70 μl of 1.0mg/ml F-actin solution containing 0.1M KCl, 5mM Tris-HCl (pH 8.0) and 0.2mM ATP was placed on a glass slide at room temperature. The sample was dried under normal atmospheric conditions for about 5 hrs. Image, bright-field. Scale bar, 10.0 mm. Salt crystal region and the periphery of the dried sample are indicated with arrows i) and ii), respectively. (b) A magnified view under phase-contrast microscope of the portion surrounded by a white square with an arrow iii) in (a) shows the concentric circular striation. Scale bar, 30 μm.
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f1-7_11: General view of the two-dimensional texture obtained by natural drying of F-actin solution. (a) About 70 μl of 1.0mg/ml F-actin solution containing 0.1M KCl, 5mM Tris-HCl (pH 8.0) and 0.2mM ATP was placed on a glass slide at room temperature. The sample was dried under normal atmospheric conditions for about 5 hrs. Image, bright-field. Scale bar, 10.0 mm. Salt crystal region and the periphery of the dried sample are indicated with arrows i) and ii), respectively. (b) A magnified view under phase-contrast microscope of the portion surrounded by a white square with an arrow iii) in (a) shows the concentric circular striation. Scale bar, 30 μm.

Mentions: Two-dimensional patterns of F-actin were formed by the following two methods: 1) natural drying of an F-actin solution (0.4 to 2.0mg/ml) or (2) incubation in a small chamber without drying of an F-actin solution (10mg/ml). In the method 1, a droplet of 40–70 μl solution was dropped from about 2 cm above the pre-cleaned glass slide (No. 1, Matsunami, Tokyo) to form a circular shaped spread sample of 10–15mm in diameter. After evaporation of water from the periphery, a two-dimensional pattern having a concentric striation of a few μm to 20–40 μm was formed around the crystallized salt that was finally formed at the center of the circularly spread sample (cf. Fig. 1a). In the method 2, the F-actin solution was put in a covered cylindrical chamber (about 5mm in diameter and about 2mm in depth), and then incubated for a week at room temperature in an ordinary atmospheric conditions.


Two-dimensional periodic texture of actin filaments formed upon drying
General view of the two-dimensional texture obtained by natural drying of F-actin solution. (a) About 70 μl of 1.0mg/ml F-actin solution containing 0.1M KCl, 5mM Tris-HCl (pH 8.0) and 0.2mM ATP was placed on a glass slide at room temperature. The sample was dried under normal atmospheric conditions for about 5 hrs. Image, bright-field. Scale bar, 10.0 mm. Salt crystal region and the periphery of the dried sample are indicated with arrows i) and ii), respectively. (b) A magnified view under phase-contrast microscope of the portion surrounded by a white square with an arrow iii) in (a) shows the concentric circular striation. Scale bar, 30 μm.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5036779&req=5

f1-7_11: General view of the two-dimensional texture obtained by natural drying of F-actin solution. (a) About 70 μl of 1.0mg/ml F-actin solution containing 0.1M KCl, 5mM Tris-HCl (pH 8.0) and 0.2mM ATP was placed on a glass slide at room temperature. The sample was dried under normal atmospheric conditions for about 5 hrs. Image, bright-field. Scale bar, 10.0 mm. Salt crystal region and the periphery of the dried sample are indicated with arrows i) and ii), respectively. (b) A magnified view under phase-contrast microscope of the portion surrounded by a white square with an arrow iii) in (a) shows the concentric circular striation. Scale bar, 30 μm.
Mentions: Two-dimensional patterns of F-actin were formed by the following two methods: 1) natural drying of an F-actin solution (0.4 to 2.0mg/ml) or (2) incubation in a small chamber without drying of an F-actin solution (10mg/ml). In the method 1, a droplet of 40–70 μl solution was dropped from about 2 cm above the pre-cleaned glass slide (No. 1, Matsunami, Tokyo) to form a circular shaped spread sample of 10–15mm in diameter. After evaporation of water from the periphery, a two-dimensional pattern having a concentric striation of a few μm to 20–40 μm was formed around the crystallized salt that was finally formed at the center of the circularly spread sample (cf. Fig. 1a). In the method 2, the F-actin solution was put in a covered cylindrical chamber (about 5mm in diameter and about 2mm in depth), and then incubated for a week at room temperature in an ordinary atmospheric conditions.

View Article: PubMed Central - PubMed

ABSTRACT

We found that a solution of actin filaments can form a periodic texture in the process of drying on a flat glass surface in the air; the periodic texture was composed of smooth meandering bundles of actin filaments. We also found that a branched salt crystal grows in the space between the meandering bundles of actin filaments. The distance between the adjacent striae (striation period) in the resulting dried two-dimensional pattern of striation decreased from about 50 to 2 μm, as the ambient temperature was increased from 4 to 40°C at 1 mg/ml actin, and showed an increasing tendency from a few to several tens μm with the increase in the initial concentration of actin filaments from 0.6 to 2.0mg/ml at room temperature. As the speed of drying is increased at a certain temperature, the striation period was also found to decrease. We propose that the formation of the two-dimensional striation pattern of bundles of actin filaments is the result of condensation of proteins due to dehydration, and suggest that the solvent flow from the center to the periphery of the sample causes the meandering of actin filaments.

No MeSH data available.


Related in: MedlinePlus