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Staurosporine induces lamellipodial widening in locomoting fish keratocytes by abolishing the gradient from radial extension of leading edge

View Article: PubMed Central - PubMed

ABSTRACT

Fish epidermal keratocytes locomote along surfaces without overall cell size or shape changes, as kinematically described by the graded radial extension (GRE) model. We found that the cell size increased during locomotion after the addition of a low dose of staurosporine or K-252a, broad-spectrum protein-serine/threonine kinase inhibitors. Quantitative shape analysis showed that the cell size increase resulted from an increase in lamellipodial width, the maximal length perpendicular to the direction of the cell locomotion, whereas the lamellipodial length, along the locomotion direction, remained constant. Importantly, the gradient of radial extension in the leading edge disappeared during lamellipodial width increase. These results suggest that a special mechanism for producing graded radial extension of lamellipodia exists to keep cell size constant, and that a protein-serine/threonine kinase plays an important role in regulating this mechanism.

No MeSH data available.


Models for illustrating keratocyte locomotion. (A) GRE model. Since leading edge extension and cell rear retraction occur perpendicularly to the cell margin at graded speeds (red arrows), cell sizes and shapes are maintained at their subsequent position (red margin). (B) Without a gradient in the extension speeds of the leading edge, cell sizes increase during locomotion.
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f5-7_69: Models for illustrating keratocyte locomotion. (A) GRE model. Since leading edge extension and cell rear retraction occur perpendicularly to the cell margin at graded speeds (red arrows), cell sizes and shapes are maintained at their subsequent position (red margin). (B) Without a gradient in the extension speeds of the leading edge, cell sizes increase during locomotion.

Mentions: As shown in Fig. 5, this phenomenon can be interpreted as the “gradient” of the radial extension of the lamellipod having been abolished in the GRE model. In fact, morphological analysis clearly showed the disappearance of the gradient from the edge extension speeds along leading edges during the widening phases (Fig. 3).


Staurosporine induces lamellipodial widening in locomoting fish keratocytes by abolishing the gradient from radial extension of leading edge
Models for illustrating keratocyte locomotion. (A) GRE model. Since leading edge extension and cell rear retraction occur perpendicularly to the cell margin at graded speeds (red arrows), cell sizes and shapes are maintained at their subsequent position (red margin). (B) Without a gradient in the extension speeds of the leading edge, cell sizes increase during locomotion.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5036771&req=5

f5-7_69: Models for illustrating keratocyte locomotion. (A) GRE model. Since leading edge extension and cell rear retraction occur perpendicularly to the cell margin at graded speeds (red arrows), cell sizes and shapes are maintained at their subsequent position (red margin). (B) Without a gradient in the extension speeds of the leading edge, cell sizes increase during locomotion.
Mentions: As shown in Fig. 5, this phenomenon can be interpreted as the “gradient” of the radial extension of the lamellipod having been abolished in the GRE model. In fact, morphological analysis clearly showed the disappearance of the gradient from the edge extension speeds along leading edges during the widening phases (Fig. 3).

View Article: PubMed Central - PubMed

ABSTRACT

Fish epidermal keratocytes locomote along surfaces without overall cell size or shape changes, as kinematically described by the graded radial extension (GRE) model. We found that the cell size increased during locomotion after the addition of a low dose of staurosporine or K-252a, broad-spectrum protein-serine/threonine kinase inhibitors. Quantitative shape analysis showed that the cell size increase resulted from an increase in lamellipodial width, the maximal length perpendicular to the direction of the cell locomotion, whereas the lamellipodial length, along the locomotion direction, remained constant. Importantly, the gradient of radial extension in the leading edge disappeared during lamellipodial width increase. These results suggest that a special mechanism for producing graded radial extension of lamellipodia exists to keep cell size constant, and that a protein-serine/threonine kinase plays an important role in regulating this mechanism.

No MeSH data available.