Limits...
Staurosporine induces lamellipodial widening in locomoting fish keratocytes by abolishing the gradient from radial extension of leading edge

View Article: PubMed Central - PubMed

ABSTRACT

Fish epidermal keratocytes locomote along surfaces without overall cell size or shape changes, as kinematically described by the graded radial extension (GRE) model. We found that the cell size increased during locomotion after the addition of a low dose of staurosporine or K-252a, broad-spectrum protein-serine/threonine kinase inhibitors. Quantitative shape analysis showed that the cell size increase resulted from an increase in lamellipodial width, the maximal length perpendicular to the direction of the cell locomotion, whereas the lamellipodial length, along the locomotion direction, remained constant. Importantly, the gradient of radial extension in the leading edge disappeared during lamellipodial width increase. These results suggest that a special mechanism for producing graded radial extension of lamellipodia exists to keep cell size constant, and that a protein-serine/threonine kinase plays an important role in regulating this mechanism.

No MeSH data available.


Transition between stationary and widening phases. Four successive outlines of a half-lamellipod separated by 12-s intervals are superimposed and colored from blue to red to represent time. Data for the same cell in as Fig. 2B–E, Fig. 3 and Supplementary Movie 3 are shown. (A) From stationary to widening phase. (B) From widening to stationary phase (outlines are mirrored for to allow comparison with A). Note that morphological changes in lamellipodial ends occurred as a result of extension speed changes along leading edges.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC5036771&req=5

f4-7_69: Transition between stationary and widening phases. Four successive outlines of a half-lamellipod separated by 12-s intervals are superimposed and colored from blue to red to represent time. Data for the same cell in as Fig. 2B–E, Fig. 3 and Supplementary Movie 3 are shown. (A) From stationary to widening phase. (B) From widening to stationary phase (outlines are mirrored for to allow comparison with A). Note that morphological changes in lamellipodial ends occurred as a result of extension speed changes along leading edges.

Mentions: Detailed observation of the transition processes between the stationary and widening phases revealed that morphological changes in lamellipodial ends occurred by extension speed changes along leading edges with no retraction of leading edges detected (Fig. 4).


Staurosporine induces lamellipodial widening in locomoting fish keratocytes by abolishing the gradient from radial extension of leading edge
Transition between stationary and widening phases. Four successive outlines of a half-lamellipod separated by 12-s intervals are superimposed and colored from blue to red to represent time. Data for the same cell in as Fig. 2B–E, Fig. 3 and Supplementary Movie 3 are shown. (A) From stationary to widening phase. (B) From widening to stationary phase (outlines are mirrored for to allow comparison with A). Note that morphological changes in lamellipodial ends occurred as a result of extension speed changes along leading edges.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC5036771&req=5

f4-7_69: Transition between stationary and widening phases. Four successive outlines of a half-lamellipod separated by 12-s intervals are superimposed and colored from blue to red to represent time. Data for the same cell in as Fig. 2B–E, Fig. 3 and Supplementary Movie 3 are shown. (A) From stationary to widening phase. (B) From widening to stationary phase (outlines are mirrored for to allow comparison with A). Note that morphological changes in lamellipodial ends occurred as a result of extension speed changes along leading edges.
Mentions: Detailed observation of the transition processes between the stationary and widening phases revealed that morphological changes in lamellipodial ends occurred by extension speed changes along leading edges with no retraction of leading edges detected (Fig. 4).

View Article: PubMed Central - PubMed

ABSTRACT

Fish epidermal keratocytes locomote along surfaces without overall cell size or shape changes, as kinematically described by the graded radial extension (GRE) model. We found that the cell size increased during locomotion after the addition of a low dose of staurosporine or K-252a, broad-spectrum protein-serine/threonine kinase inhibitors. Quantitative shape analysis showed that the cell size increase resulted from an increase in lamellipodial width, the maximal length perpendicular to the direction of the cell locomotion, whereas the lamellipodial length, along the locomotion direction, remained constant. Importantly, the gradient of radial extension in the leading edge disappeared during lamellipodial width increase. These results suggest that a special mechanism for producing graded radial extension of lamellipodia exists to keep cell size constant, and that a protein-serine/threonine kinase plays an important role in regulating this mechanism.

No MeSH data available.