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Determination of Histamine in Silages Using Nanomaghemite Core ( γ -Fe 2 O 3 )-Titanium Dioxide Shell Nanoparticles Off-Line Coupled with Ion Exchange Chromatography

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ABSTRACT

The presence of biogenic amines is a hallmark of degraded food and its products. Herein, we focused on the utilization of magnetic nanoparticles off-line coupled with ion exchange chromatography with post-column ninhydrin derivatization and Vis detection for histamine (Him) separation and detection. Primarily, we described the synthesis of magnetic nanoparticles with nanomaghemite core (γ-Fe2O3) functionalized with titanium dioxide and, then, applied these particles to specific isolation of Him. To obtain further insight into interactions between paramagnetic particles’ (PMP) surface and Him, a scanning electron microscope was employed. It was shown that binding of histamine causes an increase of relative current response of deprotonated PMPs, which confirmed formation of Him-PMPs clusters. The recovery of the isolation showed that titanium dioxide-based particles were able to bind and preconcentrate Him with recovery exceeding 90%. Finally, we successfully carried out the analyses of real samples obtained from silage. We can conclude that our modified particles are suitable for Him isolation, and thus may serve as the first isolation step of Him from biological samples, as it is demonstrated on alfalfa seed variety Tereza silage.

No MeSH data available.


Related in: MedlinePlus

(A) Scheme of isolation of biogenic amines from silage sample of PMPs; (B) Typical chromatograms overlay: red—Samples of silage bound on PMPs and green—Sample of silage before isolation on PMPs; (C) Saturation curve measured under the optimal conditions within tested concentration range 2, 4, 8, 12, and 16 mg·mL−1 of PMPs.
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ijerph-13-00904-f004: (A) Scheme of isolation of biogenic amines from silage sample of PMPs; (B) Typical chromatograms overlay: red—Samples of silage bound on PMPs and green—Sample of silage before isolation on PMPs; (C) Saturation curve measured under the optimal conditions within tested concentration range 2, 4, 8, 12, and 16 mg·mL−1 of PMPs.

Mentions: Prior to the analysis of real samples, the method for the isolation of Him by PMPs MAN18 and for its detection by subsequent IEC analysis was validated. The analytical parameters of this method are shown in Table 2. To test the ability of PMPs to serve as an isolation tool applicable for analysis of real samples without pre-treatment, we decided to use samples of silage. More detailed information about silage samples (locality and concentration) is shown in Table 3. Scheme of BAs isolation from silage samples is shown in Figure 4A. Briefly, to the samples of silage (300 mg dry sample weight) 2 mL of ACS water were added. Subsequently, the samples were incubated at 37 °C, 300 rpm, 60 min in thermoblock. After the incubation, samples were centrifuged at 2500g, 4 °C, 20 min. Supernatants were pipetted and applied to PMPs directly (250 µL), and the optimized isolation steps according to Figure 4A were carried out.


Determination of Histamine in Silages Using Nanomaghemite Core ( γ -Fe 2 O 3 )-Titanium Dioxide Shell Nanoparticles Off-Line Coupled with Ion Exchange Chromatography
(A) Scheme of isolation of biogenic amines from silage sample of PMPs; (B) Typical chromatograms overlay: red—Samples of silage bound on PMPs and green—Sample of silage before isolation on PMPs; (C) Saturation curve measured under the optimal conditions within tested concentration range 2, 4, 8, 12, and 16 mg·mL−1 of PMPs.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036737&req=5

ijerph-13-00904-f004: (A) Scheme of isolation of biogenic amines from silage sample of PMPs; (B) Typical chromatograms overlay: red—Samples of silage bound on PMPs and green—Sample of silage before isolation on PMPs; (C) Saturation curve measured under the optimal conditions within tested concentration range 2, 4, 8, 12, and 16 mg·mL−1 of PMPs.
Mentions: Prior to the analysis of real samples, the method for the isolation of Him by PMPs MAN18 and for its detection by subsequent IEC analysis was validated. The analytical parameters of this method are shown in Table 2. To test the ability of PMPs to serve as an isolation tool applicable for analysis of real samples without pre-treatment, we decided to use samples of silage. More detailed information about silage samples (locality and concentration) is shown in Table 3. Scheme of BAs isolation from silage samples is shown in Figure 4A. Briefly, to the samples of silage (300 mg dry sample weight) 2 mL of ACS water were added. Subsequently, the samples were incubated at 37 °C, 300 rpm, 60 min in thermoblock. After the incubation, samples were centrifuged at 2500g, 4 °C, 20 min. Supernatants were pipetted and applied to PMPs directly (250 µL), and the optimized isolation steps according to Figure 4A were carried out.

View Article: PubMed Central - PubMed

ABSTRACT

The presence of biogenic amines is a hallmark of degraded food and its products. Herein, we focused on the utilization of magnetic nanoparticles off-line coupled with ion exchange chromatography with post-column ninhydrin derivatization and Vis detection for histamine (Him) separation and detection. Primarily, we described the synthesis of magnetic nanoparticles with nanomaghemite core (γ-Fe2O3) functionalized with titanium dioxide and, then, applied these particles to specific isolation of Him. To obtain further insight into interactions between paramagnetic particles’ (PMP) surface and Him, a scanning electron microscope was employed. It was shown that binding of histamine causes an increase of relative current response of deprotonated PMPs, which confirmed formation of Him-PMPs clusters. The recovery of the isolation showed that titanium dioxide-based particles were able to bind and preconcentrate Him with recovery exceeding 90%. Finally, we successfully carried out the analyses of real samples obtained from silage. We can conclude that our modified particles are suitable for Him isolation, and thus may serve as the first isolation step of Him from biological samples, as it is demonstrated on alfalfa seed variety Tereza silage.

No MeSH data available.


Related in: MedlinePlus