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Determination of Histamine in Silages Using Nanomaghemite Core ( γ -Fe 2 O 3 )-Titanium Dioxide Shell Nanoparticles Off-Line Coupled with Ion Exchange Chromatography

View Article: PubMed Central - PubMed

ABSTRACT

The presence of biogenic amines is a hallmark of degraded food and its products. Herein, we focused on the utilization of magnetic nanoparticles off-line coupled with ion exchange chromatography with post-column ninhydrin derivatization and Vis detection for histamine (Him) separation and detection. Primarily, we described the synthesis of magnetic nanoparticles with nanomaghemite core (γ-Fe2O3) functionalized with titanium dioxide and, then, applied these particles to specific isolation of Him. To obtain further insight into interactions between paramagnetic particles’ (PMP) surface and Him, a scanning electron microscope was employed. It was shown that binding of histamine causes an increase of relative current response of deprotonated PMPs, which confirmed formation of Him-PMPs clusters. The recovery of the isolation showed that titanium dioxide-based particles were able to bind and preconcentrate Him with recovery exceeding 90%. Finally, we successfully carried out the analyses of real samples obtained from silage. We can conclude that our modified particles are suitable for Him isolation, and thus may serve as the first isolation step of Him from biological samples, as it is demonstrated on alfalfa seed variety Tereza silage.

No MeSH data available.


Basic characterization of prepared PMPs. (A) Micrograph, expressing microparticles surface and morphology, the length of scale bar is 200 nm; (B) XPS narrow scan of Ti2p of MAN18; (C) Binding specificity of the prepared particles after application of 100 µg·mL−1 of selected Bas. Chromatograms showing various retention times of BAs immobilized on PMPs, with expression of their peak area representing the amount specifically bound on PMPs. Scanning electrochemical microscopy 3D images characterizing the PMP electrochemical surface changes after binding of Him, relative current response of PMP surface: (D) for blank PMPs without Him bound to their surface and (E) for PMPs after binding of Him.
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ijerph-13-00904-f003: Basic characterization of prepared PMPs. (A) Micrograph, expressing microparticles surface and morphology, the length of scale bar is 200 nm; (B) XPS narrow scan of Ti2p of MAN18; (C) Binding specificity of the prepared particles after application of 100 µg·mL−1 of selected Bas. Chromatograms showing various retention times of BAs immobilized on PMPs, with expression of their peak area representing the amount specifically bound on PMPs. Scanning electrochemical microscopy 3D images characterizing the PMP electrochemical surface changes after binding of Him, relative current response of PMP surface: (D) for blank PMPs without Him bound to their surface and (E) for PMPs after binding of Him.

Mentions: An issue of derivatization methods carries drawbacks such as time-consuming and laborious sample preparation, interference from by-products, long analysis times, and the risk of indeterminate errors [28,29]. For this reason, we decided to use the PMP separation approach with the ability to increase the selectivity of the isolation of the analyte and increase the sensitivity of detection. For the modification of maghemite surface, a reaction with titanium (IV) isopropoxide in methanol water solution was used. During the reaction, titanium (IV) isopropoxide is slowly hydrolyzed and titanium dioxide nanoparticles are formed on the surface of maghemite core. The morphology of resulting product is shown in SEM micrograph in Figure 3A. The presence of Ti on magnetic particles was confirmed by XRF analysis (Figure S1). Further, the particles were characterized using XPS and XRD. XPS survey spectrum is shown in Figure S2.


Determination of Histamine in Silages Using Nanomaghemite Core ( γ -Fe 2 O 3 )-Titanium Dioxide Shell Nanoparticles Off-Line Coupled with Ion Exchange Chromatography
Basic characterization of prepared PMPs. (A) Micrograph, expressing microparticles surface and morphology, the length of scale bar is 200 nm; (B) XPS narrow scan of Ti2p of MAN18; (C) Binding specificity of the prepared particles after application of 100 µg·mL−1 of selected Bas. Chromatograms showing various retention times of BAs immobilized on PMPs, with expression of their peak area representing the amount specifically bound on PMPs. Scanning electrochemical microscopy 3D images characterizing the PMP electrochemical surface changes after binding of Him, relative current response of PMP surface: (D) for blank PMPs without Him bound to their surface and (E) for PMPs after binding of Him.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036737&req=5

ijerph-13-00904-f003: Basic characterization of prepared PMPs. (A) Micrograph, expressing microparticles surface and morphology, the length of scale bar is 200 nm; (B) XPS narrow scan of Ti2p of MAN18; (C) Binding specificity of the prepared particles after application of 100 µg·mL−1 of selected Bas. Chromatograms showing various retention times of BAs immobilized on PMPs, with expression of their peak area representing the amount specifically bound on PMPs. Scanning electrochemical microscopy 3D images characterizing the PMP electrochemical surface changes after binding of Him, relative current response of PMP surface: (D) for blank PMPs without Him bound to their surface and (E) for PMPs after binding of Him.
Mentions: An issue of derivatization methods carries drawbacks such as time-consuming and laborious sample preparation, interference from by-products, long analysis times, and the risk of indeterminate errors [28,29]. For this reason, we decided to use the PMP separation approach with the ability to increase the selectivity of the isolation of the analyte and increase the sensitivity of detection. For the modification of maghemite surface, a reaction with titanium (IV) isopropoxide in methanol water solution was used. During the reaction, titanium (IV) isopropoxide is slowly hydrolyzed and titanium dioxide nanoparticles are formed on the surface of maghemite core. The morphology of resulting product is shown in SEM micrograph in Figure 3A. The presence of Ti on magnetic particles was confirmed by XRF analysis (Figure S1). Further, the particles were characterized using XPS and XRD. XPS survey spectrum is shown in Figure S2.

View Article: PubMed Central - PubMed

ABSTRACT

The presence of biogenic amines is a hallmark of degraded food and its products. Herein, we focused on the utilization of magnetic nanoparticles off-line coupled with ion exchange chromatography with post-column ninhydrin derivatization and Vis detection for histamine (Him) separation and detection. Primarily, we described the synthesis of magnetic nanoparticles with nanomaghemite core (γ-Fe2O3) functionalized with titanium dioxide and, then, applied these particles to specific isolation of Him. To obtain further insight into interactions between paramagnetic particles’ (PMP) surface and Him, a scanning electron microscope was employed. It was shown that binding of histamine causes an increase of relative current response of deprotonated PMPs, which confirmed formation of Him-PMPs clusters. The recovery of the isolation showed that titanium dioxide-based particles were able to bind and preconcentrate Him with recovery exceeding 90%. Finally, we successfully carried out the analyses of real samples obtained from silage. We can conclude that our modified particles are suitable for Him isolation, and thus may serve as the first isolation step of Him from biological samples, as it is demonstrated on alfalfa seed variety Tereza silage.

No MeSH data available.