Limits...
Determination of Histamine in Silages Using Nanomaghemite Core ( γ -Fe 2 O 3 )-Titanium Dioxide Shell Nanoparticles Off-Line Coupled with Ion Exchange Chromatography

View Article: PubMed Central - PubMed

ABSTRACT

The presence of biogenic amines is a hallmark of degraded food and its products. Herein, we focused on the utilization of magnetic nanoparticles off-line coupled with ion exchange chromatography with post-column ninhydrin derivatization and Vis detection for histamine (Him) separation and detection. Primarily, we described the synthesis of magnetic nanoparticles with nanomaghemite core (γ-Fe2O3) functionalized with titanium dioxide and, then, applied these particles to specific isolation of Him. To obtain further insight into interactions between paramagnetic particles’ (PMP) surface and Him, a scanning electron microscope was employed. It was shown that binding of histamine causes an increase of relative current response of deprotonated PMPs, which confirmed formation of Him-PMPs clusters. The recovery of the isolation showed that titanium dioxide-based particles were able to bind and preconcentrate Him with recovery exceeding 90%. Finally, we successfully carried out the analyses of real samples obtained from silage. We can conclude that our modified particles are suitable for Him isolation, and thus may serve as the first isolation step of Him from biological samples, as it is demonstrated on alfalfa seed variety Tereza silage.

No MeSH data available.


(A) The chemical structure of studied biogenic amines; (B) The scheme of amines oxidation in plants, their effects on damaging of membranes and defense mechanisms. H2O2 can be removed by catalase and HO· by mannitol. The defense mechanism of GPx/GR and TPx/TR systems and nicotinamide nucleotide TH are also shown. In the scheme, GPx stays for glutathione peroxidase; GRx for glutathione reductase; MPT for mitochondrial permeability transition; TH for transhydrogenase; TPx for thioredoxin peroxidase; and TR for thioredoxin reductase. This scheme was inspired by Agostinelli et al. [2].
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5036737&req=5

ijerph-13-00904-f001: (A) The chemical structure of studied biogenic amines; (B) The scheme of amines oxidation in plants, their effects on damaging of membranes and defense mechanisms. H2O2 can be removed by catalase and HO· by mannitol. The defense mechanism of GPx/GR and TPx/TR systems and nicotinamide nucleotide TH are also shown. In the scheme, GPx stays for glutathione peroxidase; GRx for glutathione reductase; MPT for mitochondrial permeability transition; TH for transhydrogenase; TPx for thioredoxin peroxidase; and TR for thioredoxin reductase. This scheme was inspired by Agostinelli et al. [2].

Mentions: The occurrence of biogenic amines (BAs) is considered as an important indicator of freshness and quality of food [1]. Therefore, it is not surprising that BAs attract an attention of various branches from chemists to food technologists. From the chemical point of view, BAs are basic, nitrogenous, low molecular-mass compounds formed as products of physiological metabolic activities of microorganisms, plants, and animals [2]. BAs can have aliphatic (e.g., spermine, spermidine, putrescine, cadaverine), heterocyclic (e.g., tryptamine, histamine), or aromatic (e.g., tyramine) structure derived mainly from the decarboxylation of amino acids [3], whereas the chemical structures of the selected BAs can be found in Figure 1A. The most amines occurring in foods originate from the corresponding amino acids, which have undergone decarboxylation by putrefactive bacteria, producing mainly putrescine (Put), Cadaverine (Cad), and histamine (Him) or lactic acid bacteria producing mainly tyramine (Tym) [4]. To produce Him, the bacteria have to express histidine decarboxylase.


Determination of Histamine in Silages Using Nanomaghemite Core ( γ -Fe 2 O 3 )-Titanium Dioxide Shell Nanoparticles Off-Line Coupled with Ion Exchange Chromatography
(A) The chemical structure of studied biogenic amines; (B) The scheme of amines oxidation in plants, their effects on damaging of membranes and defense mechanisms. H2O2 can be removed by catalase and HO· by mannitol. The defense mechanism of GPx/GR and TPx/TR systems and nicotinamide nucleotide TH are also shown. In the scheme, GPx stays for glutathione peroxidase; GRx for glutathione reductase; MPT for mitochondrial permeability transition; TH for transhydrogenase; TPx for thioredoxin peroxidase; and TR for thioredoxin reductase. This scheme was inspired by Agostinelli et al. [2].
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036737&req=5

ijerph-13-00904-f001: (A) The chemical structure of studied biogenic amines; (B) The scheme of amines oxidation in plants, their effects on damaging of membranes and defense mechanisms. H2O2 can be removed by catalase and HO· by mannitol. The defense mechanism of GPx/GR and TPx/TR systems and nicotinamide nucleotide TH are also shown. In the scheme, GPx stays for glutathione peroxidase; GRx for glutathione reductase; MPT for mitochondrial permeability transition; TH for transhydrogenase; TPx for thioredoxin peroxidase; and TR for thioredoxin reductase. This scheme was inspired by Agostinelli et al. [2].
Mentions: The occurrence of biogenic amines (BAs) is considered as an important indicator of freshness and quality of food [1]. Therefore, it is not surprising that BAs attract an attention of various branches from chemists to food technologists. From the chemical point of view, BAs are basic, nitrogenous, low molecular-mass compounds formed as products of physiological metabolic activities of microorganisms, plants, and animals [2]. BAs can have aliphatic (e.g., spermine, spermidine, putrescine, cadaverine), heterocyclic (e.g., tryptamine, histamine), or aromatic (e.g., tyramine) structure derived mainly from the decarboxylation of amino acids [3], whereas the chemical structures of the selected BAs can be found in Figure 1A. The most amines occurring in foods originate from the corresponding amino acids, which have undergone decarboxylation by putrefactive bacteria, producing mainly putrescine (Put), Cadaverine (Cad), and histamine (Him) or lactic acid bacteria producing mainly tyramine (Tym) [4]. To produce Him, the bacteria have to express histidine decarboxylase.

View Article: PubMed Central - PubMed

ABSTRACT

The presence of biogenic amines is a hallmark of degraded food and its products. Herein, we focused on the utilization of magnetic nanoparticles off-line coupled with ion exchange chromatography with post-column ninhydrin derivatization and Vis detection for histamine (Him) separation and detection. Primarily, we described the synthesis of magnetic nanoparticles with nanomaghemite core (γ-Fe2O3) functionalized with titanium dioxide and, then, applied these particles to specific isolation of Him. To obtain further insight into interactions between paramagnetic particles’ (PMP) surface and Him, a scanning electron microscope was employed. It was shown that binding of histamine causes an increase of relative current response of deprotonated PMPs, which confirmed formation of Him-PMPs clusters. The recovery of the isolation showed that titanium dioxide-based particles were able to bind and preconcentrate Him with recovery exceeding 90%. Finally, we successfully carried out the analyses of real samples obtained from silage. We can conclude that our modified particles are suitable for Him isolation, and thus may serve as the first isolation step of Him from biological samples, as it is demonstrated on alfalfa seed variety Tereza silage.

No MeSH data available.