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Biodegradation of Methyl tert -Butyl Ether by Co-Metabolism with a Pseudomonas sp. Strain

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ABSTRACT

Co-metabolic bioremediation is supposed to be an impressive and promising approach in the elimination technology of methyl tert-butyl ether (MTBE), which was found to be a common pollutant worldwide in the ground or underground water in recent years. In this paper, bacterial strain DZ13 (which can co-metabolically degrade MTBE) was isolated and named as Pseudomonas sp. DZ13 based on the result of 16S rRNA gene sequencing analysis. Strain DZ13 could grow on n-alkanes (C5-C8), accompanied with the co-metabolic degradation of MTBE. Diverse n-alkanes with different carbon number showed a significant influence on the degradation rate of MTBE and accumulation of tert-butyl alcohol (TBA). When Pseudomonas sp. DZ13 co-metabolically degraded MTBE with n-pentane as the growth substrate, a higher MTBE-degrading rate (Vmax = 38.1 nmol/min/mgprotein, Ks = 6.8 mmol/L) and lower TBA-accumulation was observed. In the continuous degradation experiment, the removal efficiency of MTBE by Pseudomonas sp. Strain DZ13 did not show an obvious decrease after five times of continuous addition.

No MeSH data available.


Co-metabolic degradation of MTBE with n-pentane as growth substrate. Vertical arrows indicate the additions of MTBE and n-pentane. MTBE (10 mg/L) and n-pentane (50 mg/L) were re-spiked when the residual concentrations of both MTBE and n-pentane in the cultures were below 5% of the initial concentrations. All of the values are the means of triplicate.
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ijerph-13-00883-f003: Co-metabolic degradation of MTBE with n-pentane as growth substrate. Vertical arrows indicate the additions of MTBE and n-pentane. MTBE (10 mg/L) and n-pentane (50 mg/L) were re-spiked when the residual concentrations of both MTBE and n-pentane in the cultures were below 5% of the initial concentrations. All of the values are the means of triplicate.

Mentions: Further research on the co-metabolic degradation of MTBE with n-pentane as the growth substrate was conducted to determine the continuous degradation ability of strain DZ13. MTBE (10 mg/L) and n-pentane (50 mg/L) were re-spiked when the residual concentrations of both MTBE and n-pentane in the cultures were below 5% of the initial concentrations. Figure 3 showed the degradation curves of MTBE and n-pentane after repeated feeding and degrading for five times. Pure strain DZ13 can co-metabolically degrade MTBE (10 mg/L) at the rate of 45.3–52.5 mgMTBE/gprotein/h with n-octane (50 mg/L) as the growth substrate. An obvious lag phase was observed in the MTBE-degradation curve compared to the n-octane-degradation curve, in which n-pentane was transformed immediately once it was added. The first degradation circle of MTBE and n-octane was completed in two days. After being re-spiked, n-pentane was degraded continuously, accompanied by the removal of MTBE. It has been observed that the MTBE degradation rate did not apparently decrease after the fifth addition.


Biodegradation of Methyl tert -Butyl Ether by Co-Metabolism with a Pseudomonas sp. Strain
Co-metabolic degradation of MTBE with n-pentane as growth substrate. Vertical arrows indicate the additions of MTBE and n-pentane. MTBE (10 mg/L) and n-pentane (50 mg/L) were re-spiked when the residual concentrations of both MTBE and n-pentane in the cultures were below 5% of the initial concentrations. All of the values are the means of triplicate.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036716&req=5

ijerph-13-00883-f003: Co-metabolic degradation of MTBE with n-pentane as growth substrate. Vertical arrows indicate the additions of MTBE and n-pentane. MTBE (10 mg/L) and n-pentane (50 mg/L) were re-spiked when the residual concentrations of both MTBE and n-pentane in the cultures were below 5% of the initial concentrations. All of the values are the means of triplicate.
Mentions: Further research on the co-metabolic degradation of MTBE with n-pentane as the growth substrate was conducted to determine the continuous degradation ability of strain DZ13. MTBE (10 mg/L) and n-pentane (50 mg/L) were re-spiked when the residual concentrations of both MTBE and n-pentane in the cultures were below 5% of the initial concentrations. Figure 3 showed the degradation curves of MTBE and n-pentane after repeated feeding and degrading for five times. Pure strain DZ13 can co-metabolically degrade MTBE (10 mg/L) at the rate of 45.3–52.5 mgMTBE/gprotein/h with n-octane (50 mg/L) as the growth substrate. An obvious lag phase was observed in the MTBE-degradation curve compared to the n-octane-degradation curve, in which n-pentane was transformed immediately once it was added. The first degradation circle of MTBE and n-octane was completed in two days. After being re-spiked, n-pentane was degraded continuously, accompanied by the removal of MTBE. It has been observed that the MTBE degradation rate did not apparently decrease after the fifth addition.

View Article: PubMed Central - PubMed

ABSTRACT

Co-metabolic bioremediation is supposed to be an impressive and promising approach in the elimination technology of methyl tert-butyl ether (MTBE), which was found to be a common pollutant worldwide in the ground or underground water in recent years. In this paper, bacterial strain DZ13 (which can co-metabolically degrade MTBE) was isolated and named as Pseudomonas sp. DZ13 based on the result of 16S rRNA gene sequencing analysis. Strain DZ13 could grow on n-alkanes (C5-C8), accompanied with the co-metabolic degradation of MTBE. Diverse n-alkanes with different carbon number showed a significant influence on the degradation rate of MTBE and accumulation of tert-butyl alcohol (TBA). When Pseudomonas sp. DZ13 co-metabolically degraded MTBE with n-pentane as the growth substrate, a higher MTBE-degrading rate (Vmax = 38.1 nmol/min/mgprotein, Ks = 6.8 mmol/L) and lower TBA-accumulation was observed. In the continuous degradation experiment, the removal efficiency of MTBE by Pseudomonas sp. Strain DZ13 did not show an obvious decrease after five times of continuous addition.

No MeSH data available.