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Biodegradation of Methyl tert -Butyl Ether by Co-Metabolism with a Pseudomonas sp. Strain

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ABSTRACT

Co-metabolic bioremediation is supposed to be an impressive and promising approach in the elimination technology of methyl tert-butyl ether (MTBE), which was found to be a common pollutant worldwide in the ground or underground water in recent years. In this paper, bacterial strain DZ13 (which can co-metabolically degrade MTBE) was isolated and named as Pseudomonas sp. DZ13 based on the result of 16S rRNA gene sequencing analysis. Strain DZ13 could grow on n-alkanes (C5-C8), accompanied with the co-metabolic degradation of MTBE. Diverse n-alkanes with different carbon number showed a significant influence on the degradation rate of MTBE and accumulation of tert-butyl alcohol (TBA). When Pseudomonas sp. DZ13 co-metabolically degraded MTBE with n-pentane as the growth substrate, a higher MTBE-degrading rate (Vmax = 38.1 nmol/min/mgprotein, Ks = 6.8 mmol/L) and lower TBA-accumulation was observed. In the continuous degradation experiment, the removal efficiency of MTBE by Pseudomonas sp. Strain DZ13 did not show an obvious decrease after five times of continuous addition.

No MeSH data available.


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Phylogenetic tree of Pseudomonas sp. DZ13 based on 16S rRNA sequences. The tree is constructed by the neighbor-joining method of the bootstrap test (1000 replicates). The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. All positions containing gaps and missing data were eliminated from the dataset (Complete deletion option).
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ijerph-13-00883-f001: Phylogenetic tree of Pseudomonas sp. DZ13 based on 16S rRNA sequences. The tree is constructed by the neighbor-joining method of the bootstrap test (1000 replicates). The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. All positions containing gaps and missing data were eliminated from the dataset (Complete deletion option).

Mentions: Finally, the pure strain DZ13, which was gram-negative and short rod shaped, was selected for further study. The 16S rRNA-based sequence analysis revealed that strain DZ13 belongs to the Pseudomonas genus, and was named Pseudomonas sp. DZ13. Pseudomonas hunanensis LV was found to be phylogenetically closest to the isolated strain DZ13, showing 99% sequence similarity between the 16S rRNA genes (Figure 1). The 16S rRNA sequence of the isolated DZ13 strain was deposited in NCBI GenBank database under the accession number of KX301316.


Biodegradation of Methyl tert -Butyl Ether by Co-Metabolism with a Pseudomonas sp. Strain
Phylogenetic tree of Pseudomonas sp. DZ13 based on 16S rRNA sequences. The tree is constructed by the neighbor-joining method of the bootstrap test (1000 replicates). The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. All positions containing gaps and missing data were eliminated from the dataset (Complete deletion option).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036716&req=5

ijerph-13-00883-f001: Phylogenetic tree of Pseudomonas sp. DZ13 based on 16S rRNA sequences. The tree is constructed by the neighbor-joining method of the bootstrap test (1000 replicates). The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. All positions containing gaps and missing data were eliminated from the dataset (Complete deletion option).
Mentions: Finally, the pure strain DZ13, which was gram-negative and short rod shaped, was selected for further study. The 16S rRNA-based sequence analysis revealed that strain DZ13 belongs to the Pseudomonas genus, and was named Pseudomonas sp. DZ13. Pseudomonas hunanensis LV was found to be phylogenetically closest to the isolated strain DZ13, showing 99% sequence similarity between the 16S rRNA genes (Figure 1). The 16S rRNA sequence of the isolated DZ13 strain was deposited in NCBI GenBank database under the accession number of KX301316.

View Article: PubMed Central - PubMed

ABSTRACT

Co-metabolic bioremediation is supposed to be an impressive and promising approach in the elimination technology of methyl tert-butyl ether (MTBE), which was found to be a common pollutant worldwide in the ground or underground water in recent years. In this paper, bacterial strain DZ13 (which can co-metabolically degrade MTBE) was isolated and named as Pseudomonas sp. DZ13 based on the result of 16S rRNA gene sequencing analysis. Strain DZ13 could grow on n-alkanes (C5-C8), accompanied with the co-metabolic degradation of MTBE. Diverse n-alkanes with different carbon number showed a significant influence on the degradation rate of MTBE and accumulation of tert-butyl alcohol (TBA). When Pseudomonas sp. DZ13 co-metabolically degraded MTBE with n-pentane as the growth substrate, a higher MTBE-degrading rate (Vmax = 38.1 nmol/min/mgprotein, Ks = 6.8 mmol/L) and lower TBA-accumulation was observed. In the continuous degradation experiment, the removal efficiency of MTBE by Pseudomonas sp. Strain DZ13 did not show an obvious decrease after five times of continuous addition.

No MeSH data available.


Related in: MedlinePlus