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Spatial Variability of PAHs and Microbial Community Structure in Surrounding Surficial Soil of Coal-Fired Power Plants in Xuzhou, China

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ABSTRACT

This work investigated the spatial profile and source analysis of polycyclic aromatic hydrocarbons (PAHs) in soil that surrounds coal-fired power plants in Xuzhou, China. High-throughput sequencing was employed to investigate the composition and structure of soil bacterial communities. The total concentration of 15 PAHs in the surface soils ranged from 164.87 to 3494.81 μg/kg dry weight. The spatial profile of PAHs was site-specific with a concentration of 1400.09–3494.81 μg/kg in Yaozhuang. Based on the qualitative and principal component analysis results, coal burning and vehicle emission were found to be the main sources of PAHs in the surface soils. The phylogenetic analysis revealed differences in bacterial community compositions among different sampling sites. Proteobacteria was the most abundant phylum, while Acidobacteria was the second most abundant. The orders of Campylobacterales, Desulfobacterales and Hydrogenophilales had the most significant differences in relative abundance among the sampling sites. The redundancy analysis revealed that the differences in bacterial communities could be explained by the organic matter content. They could also be explicated by the acenaphthene concentration with longer arrows. Furthermore, OTUs of Proteobacteria phylum plotted around particular samples were confirmed to have a different composition of Proteobacteria phylum among the sample sites. Evaluating the relationship between soil PAHs concentration and bacterial community composition may provide useful information for the remediation of PAH contaminated sites.

No MeSH data available.


Redundancy analysis showed the correlation between bacterial consortia and soil environment factors (a) and some frequent OTUs of phylum Proteobacteria (b).
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ijerph-13-00878-f006: Redundancy analysis showed the correlation between bacterial consortia and soil environment factors (a) and some frequent OTUs of phylum Proteobacteria (b).

Mentions: The bacterial OTUs obtained from Illumina sequencing of 16S rRNA genes were classified into 35 different phyla or 254 orders. Figure 5a showed that Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla accounted for more than 80% of the total reads in every sample. Phyla that occupied less than 1% of the bacterial communities were not shown. Proteobacteria was the most abundant phylum (Figure 5a), ranging from 15.1% to 36.6% in the samples. Acidobacteria, the second most abundant phylum, occupied about 15.6% in all samples. In general, bacterial community structures varied among different sampling sites (Figure 5a). The trend was more evident as revealed by RDA analysis with samples that came from around the same power plants grouped nearer to each other (Figure 6).


Spatial Variability of PAHs and Microbial Community Structure in Surrounding Surficial Soil of Coal-Fired Power Plants in Xuzhou, China
Redundancy analysis showed the correlation between bacterial consortia and soil environment factors (a) and some frequent OTUs of phylum Proteobacteria (b).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036711&req=5

ijerph-13-00878-f006: Redundancy analysis showed the correlation between bacterial consortia and soil environment factors (a) and some frequent OTUs of phylum Proteobacteria (b).
Mentions: The bacterial OTUs obtained from Illumina sequencing of 16S rRNA genes were classified into 35 different phyla or 254 orders. Figure 5a showed that Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla accounted for more than 80% of the total reads in every sample. Phyla that occupied less than 1% of the bacterial communities were not shown. Proteobacteria was the most abundant phylum (Figure 5a), ranging from 15.1% to 36.6% in the samples. Acidobacteria, the second most abundant phylum, occupied about 15.6% in all samples. In general, bacterial community structures varied among different sampling sites (Figure 5a). The trend was more evident as revealed by RDA analysis with samples that came from around the same power plants grouped nearer to each other (Figure 6).

View Article: PubMed Central - PubMed

ABSTRACT

This work investigated the spatial profile and source analysis of polycyclic aromatic hydrocarbons (PAHs) in soil that surrounds coal-fired power plants in Xuzhou, China. High-throughput sequencing was employed to investigate the composition and structure of soil bacterial communities. The total concentration of 15 PAHs in the surface soils ranged from 164.87 to 3494.81 μg/kg dry weight. The spatial profile of PAHs was site-specific with a concentration of 1400.09–3494.81 μg/kg in Yaozhuang. Based on the qualitative and principal component analysis results, coal burning and vehicle emission were found to be the main sources of PAHs in the surface soils. The phylogenetic analysis revealed differences in bacterial community compositions among different sampling sites. Proteobacteria was the most abundant phylum, while Acidobacteria was the second most abundant. The orders of Campylobacterales, Desulfobacterales and Hydrogenophilales had the most significant differences in relative abundance among the sampling sites. The redundancy analysis revealed that the differences in bacterial communities could be explained by the organic matter content. They could also be explicated by the acenaphthene concentration with longer arrows. Furthermore, OTUs of Proteobacteria phylum plotted around particular samples were confirmed to have a different composition of Proteobacteria phylum among the sample sites. Evaluating the relationship between soil PAHs concentration and bacterial community composition may provide useful information for the remediation of PAH contaminated sites.

No MeSH data available.