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Photochemical and photophysical properties of carotenoid immobilized on a surfactant micellar medium including chlorophyll as an artificial photosynthesis system

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ABSTRACT

To develop an artificial photosynthesis model, the anionic water-soluble carotenoid dye crocetin was electrostatically immobilized onto the surface of cationic surfactant cetyltrimethylammonium bromide (CTAB) micellar medium including Mg chlorophyll-a and b (MgChl-a and b) (Cro/MgChl), and its photophysical properties were studied using UV-vis absorption and fluorescence spectroscopy. The fluorescence of MgChl-a and b was observed, with the excitation wavelength attributed to the absorption band of crocetin, indicating that photo-induced energy transfer from the photoexcited state of crocetin to MgChl-a and b occurs. The photostability of MgChl-a and b in Cro/MgChl was investigated under continuous irradiation. After 60 min irradiation, the absorbance decreases at 660 nm Cro/MgChl and MgChl-a/b, without crocetin, were 3.0 and 17%, respectively. These results indicate that the photo-bleaching rate of MgChl-a/b in Cro/MgChl on irradiation is suppressed by the crocetin molecule on the surface of micelles.

No MeSH data available.


Schematic representation of the preparation of Cro/MgChl.
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f1-2_57: Schematic representation of the preparation of Cro/MgChl.

Mentions: Cro/MgChl was prepared according to the method shown in Figure 1. At first, MgChl-a and b were solubilized in 20 mM cetyltrimethylammonium bromide (CTAB) micellar medium containing potassium phosphate buffer (pH 7) (procedure 1 in Fig. 1). Then, crocetin solution containing potassium phosphate buffer (pH 7) was added to the CTAB micellar solution containing MgChl-a and b (procedure 2 in Fig. 1). The concentrations of crocetin, and MgChl-a and b in Cro/MgChl were 0.43, 0.40, and 0.03 mM, respectively.


Photochemical and photophysical properties of carotenoid immobilized on a surfactant micellar medium including chlorophyll as an artificial photosynthesis system
Schematic representation of the preparation of Cro/MgChl.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC5036646&req=5

f1-2_57: Schematic representation of the preparation of Cro/MgChl.
Mentions: Cro/MgChl was prepared according to the method shown in Figure 1. At first, MgChl-a and b were solubilized in 20 mM cetyltrimethylammonium bromide (CTAB) micellar medium containing potassium phosphate buffer (pH 7) (procedure 1 in Fig. 1). Then, crocetin solution containing potassium phosphate buffer (pH 7) was added to the CTAB micellar solution containing MgChl-a and b (procedure 2 in Fig. 1). The concentrations of crocetin, and MgChl-a and b in Cro/MgChl were 0.43, 0.40, and 0.03 mM, respectively.

View Article: PubMed Central - PubMed

ABSTRACT

To develop an artificial photosynthesis model, the anionic water-soluble carotenoid dye crocetin was electrostatically immobilized onto the surface of cationic surfactant cetyltrimethylammonium bromide (CTAB) micellar medium including Mg chlorophyll-a and b (MgChl-a and b) (Cro/MgChl), and its photophysical properties were studied using UV-vis absorption and fluorescence spectroscopy. The fluorescence of MgChl-a and b was observed, with the excitation wavelength attributed to the absorption band of crocetin, indicating that photo-induced energy transfer from the photoexcited state of crocetin to MgChl-a and b occurs. The photostability of MgChl-a and b in Cro/MgChl was investigated under continuous irradiation. After 60 min irradiation, the absorbance decreases at 660 nm Cro/MgChl and MgChl-a/b, without crocetin, were 3.0 and 17%, respectively. These results indicate that the photo-bleaching rate of MgChl-a/b in Cro/MgChl on irradiation is suppressed by the crocetin molecule on the surface of micelles.

No MeSH data available.