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Applicability of thin film phase plates in biological electron microscopy

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ABSTRACT

Presented is an evaluation of phase contrast techniques in transmission electron microscopy. The traditional defocus phase contrast is compared to two recently developed phase plate techniques. One is the Zernike phase contrast transmission electron microscope, the other is the Hilbert differential contrast thransmission electron microscope. The imaging characteristics of each technique are discussed. Phase plate techniques provide improved contrast for ice-embedded biological samples which are a challenge for the conventional defocus phase contrast. The flat spectral response of the Zernike and Hilbert modes extends towards the low frequencies which are severely suppressed in the conventional defocus mode. Target applications for each of the phase contrast techniques are discussed based on the specifics of image formation and spectral transfer.

No MeSH data available.


Example of a HDC-TEM application. (a) HDC-TEM image of ice-embedded mitochondrion, defocus 1.6 μm (Matsumoto, K., Fujita, Y., Yoneda, M., Itoh, M., Tanaka, M., Danev, R. and Nagayama, K., unpublished data). (b) modulus of the Fourier transform of (a).
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f6-2_35: Example of a HDC-TEM application. (a) HDC-TEM image of ice-embedded mitochondrion, defocus 1.6 μm (Matsumoto, K., Fujita, Y., Yoneda, M., Itoh, M., Tanaka, M., Danev, R. and Nagayama, K., unpublished data). (b) modulus of the Fourier transform of (a).

Mentions: Figs. 6a, b show a practical example of HDC-TEM application. The specimen is ice-embedded mitochondrion. The topographic representation makes it easy to recognize details in the ultra structure of the subject. The direct interpretation of the data is easy due to the topographic representation and absence of defocus modulation. It must be noted that the appearance of the image do not necessarily correspond to the surface features of the sample. The relief appearance corresponds to the phase information. In the case of ice-embedded biological samples this is the density variations inside the volume.


Applicability of thin film phase plates in biological electron microscopy
Example of a HDC-TEM application. (a) HDC-TEM image of ice-embedded mitochondrion, defocus 1.6 μm (Matsumoto, K., Fujita, Y., Yoneda, M., Itoh, M., Tanaka, M., Danev, R. and Nagayama, K., unpublished data). (b) modulus of the Fourier transform of (a).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5036645&req=5

f6-2_35: Example of a HDC-TEM application. (a) HDC-TEM image of ice-embedded mitochondrion, defocus 1.6 μm (Matsumoto, K., Fujita, Y., Yoneda, M., Itoh, M., Tanaka, M., Danev, R. and Nagayama, K., unpublished data). (b) modulus of the Fourier transform of (a).
Mentions: Figs. 6a, b show a practical example of HDC-TEM application. The specimen is ice-embedded mitochondrion. The topographic representation makes it easy to recognize details in the ultra structure of the subject. The direct interpretation of the data is easy due to the topographic representation and absence of defocus modulation. It must be noted that the appearance of the image do not necessarily correspond to the surface features of the sample. The relief appearance corresponds to the phase information. In the case of ice-embedded biological samples this is the density variations inside the volume.

View Article: PubMed Central - PubMed

ABSTRACT

Presented is an evaluation of phase contrast techniques in transmission electron microscopy. The traditional defocus phase contrast is compared to two recently developed phase plate techniques. One is the Zernike phase contrast transmission electron microscope, the other is the Hilbert differential contrast thransmission electron microscope. The imaging characteristics of each technique are discussed. Phase plate techniques provide improved contrast for ice-embedded biological samples which are a challenge for the conventional defocus phase contrast. The flat spectral response of the Zernike and Hilbert modes extends towards the low frequencies which are severely suppressed in the conventional defocus mode. Target applications for each of the phase contrast techniques are discussed based on the specifics of image formation and spectral transfer.

No MeSH data available.