Limits...
Mechanisms of secondary structure breakers in soluble proteins

View Article: PubMed Central - PubMed

ABSTRACT

Breaking signals of secondary structure put strong limitations on the tertiary structures of proteins. In addition to proline and glycine clusters, which are well-known secondary structure breakers, clusters of amphiphilic residues were found to be a novel type of secondary structure breaker. These secondary structure breakers were found to depend on specific environmental factors. Such conditions included the average hydrophobicity, the helical periodicity, the density of serine and threonine residues, and the presence of tryptophan and tyrosine clusters. Principal component analysis of environmental factors was conducted in order to identify candidate breakers in the secondary structure breaking regions. Predicted breakers were located in breaking regions with an accuracy of 72%. Taking the loop core into consideration, almost 90% of the predicted breakers were located in the loop segments. When the migration effect of the breaking point was taken into account, the loop segments with the predicted breakers covered two thirds of all loop segments. Herein, the possibility of secondary structure prediction based on secondary structure breakers is discussed. The system of the present method is available at the URL: http://bp.nuap.nagoya-u.ac.jp/sosui/sosuibreaker/sosuibreaker_submit.html.

No MeSH data available.


Predicted secondary structure breakers by proline, glycine, and amphiphilic peaks: (a) myoglobin 1a6m (all α-type); (b) cytochrome-C552 1c52 (all α-type); (c) FC-γ RIIB ectodomain 2fcbA (all β-type); (d) human neutrophil gelatinase 1dfvB (all β-type).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC5036629&req=5

f8-1_55: Predicted secondary structure breakers by proline, glycine, and amphiphilic peaks: (a) myoglobin 1a6m (all α-type); (b) cytochrome-C552 1c52 (all α-type); (c) FC-γ RIIB ectodomain 2fcbA (all β-type); (d) human neutrophil gelatinase 1dfvB (all β-type).

Mentions: The present method is applicable to breakers of both α-helices and β-sheets. Figure 8 shows a number of examples. The results of all-α-type proteins, myoglobin (1a6m) and cytochrome-C552 (1c52), and all-β-type proteins, FC-γ RIIB ectodomain (2fcbA) and human neutrophil gelatinase (1dfvB) are shown in Figures 8a–d. The α-helices and the β-sheets are effectively terminated by the predicted breakers, irrespective of the types of folds.


Mechanisms of secondary structure breakers in soluble proteins
Predicted secondary structure breakers by proline, glycine, and amphiphilic peaks: (a) myoglobin 1a6m (all α-type); (b) cytochrome-C552 1c52 (all α-type); (c) FC-γ RIIB ectodomain 2fcbA (all β-type); (d) human neutrophil gelatinase 1dfvB (all β-type).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC5036629&req=5

f8-1_55: Predicted secondary structure breakers by proline, glycine, and amphiphilic peaks: (a) myoglobin 1a6m (all α-type); (b) cytochrome-C552 1c52 (all α-type); (c) FC-γ RIIB ectodomain 2fcbA (all β-type); (d) human neutrophil gelatinase 1dfvB (all β-type).
Mentions: The present method is applicable to breakers of both α-helices and β-sheets. Figure 8 shows a number of examples. The results of all-α-type proteins, myoglobin (1a6m) and cytochrome-C552 (1c52), and all-β-type proteins, FC-γ RIIB ectodomain (2fcbA) and human neutrophil gelatinase (1dfvB) are shown in Figures 8a–d. The α-helices and the β-sheets are effectively terminated by the predicted breakers, irrespective of the types of folds.

View Article: PubMed Central - PubMed

ABSTRACT

Breaking signals of secondary structure put strong limitations on the tertiary structures of proteins. In addition to proline and glycine clusters, which are well-known secondary structure breakers, clusters of amphiphilic residues were found to be a novel type of secondary structure breaker. These secondary structure breakers were found to depend on specific environmental factors. Such conditions included the average hydrophobicity, the helical periodicity, the density of serine and threonine residues, and the presence of tryptophan and tyrosine clusters. Principal component analysis of environmental factors was conducted in order to identify candidate breakers in the secondary structure breaking regions. Predicted breakers were located in breaking regions with an accuracy of 72%. Taking the loop core into consideration, almost 90% of the predicted breakers were located in the loop segments. When the migration effect of the breaking point was taken into account, the loop segments with the predicted breakers covered two thirds of all loop segments. Herein, the possibility of secondary structure prediction based on secondary structure breakers is discussed. The system of the present method is available at the URL: http://bp.nuap.nagoya-u.ac.jp/sosui/sosuibreaker/sosuibreaker_submit.html.

No MeSH data available.