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Intermolecular interaction of phosphatidylinositol with the lipid raft molecules sphingomyelin and cholesterol

View Article: PubMed Central - PubMed

ABSTRACT

Diacylphosphatidylinositol (PI) is the starting reactant in the process of phosphatidylinositide-related signal transduction mediated through the lipid raft domain. We investigated intermolecular interactions of PI with major raft components, sphingomyelin (SM) and cholesterol (Chol), using surface pressure–molecular area (π–A) isotherm measurements. The classical mean molecular area versus composition plot showed that the measured mean molecular areas are smaller in PI/Chol mixed monolayers and larger in PI/SM mixed monolayers than those calculated on the basis of the ideal additivity. These results indicate that PI interacts attractively with Chol and repulsively with SM. In addition, we energetically evaluated the interaction of PI with SM/Chol mixtures and found that the mixing energy of PI/SM/Chol ternary monolayers decreased as the molar ratio of Chol to SM increased. In order to quantitatively analyze the distribution of PI we calculated the chemical potentials of mixing of PI into the SM/Chol mixed monolayer and into the dioleoylphosphatidylcholine (DOPC) monolayer, which was used as a model for the fluid matrix, on the basis of partial molecular area analysis. Analysis using the chemical potential of mixing of PI suggested that partition of PI molecules between these two monolayers can be changed by a factor of about 1.7 in response to change in Chol molar fraction in the SM/Chol mixed monolayer from 0.3 to 0.6 when the concentration of PI in the DOPC monolayer is kept constant at 7 mol%.

No MeSH data available.


Excess mixing energy of PI and SM/Chol mixtures (SCm), ΔGexSCm, as a function of XPI. The value of ΔGexSCm was calculated by integration of area deviation (ΔA) over the surface pressure (Eq. (7)). The molar ratios of Chol in the SCm, rChol, are 0 (filled inverted triangle), 0.3 (filled square), 0.6 (filled circle), 0.9 (filled diamond) and 1.0 (filled triangle).
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f4-4_1: Excess mixing energy of PI and SM/Chol mixtures (SCm), ΔGexSCm, as a function of XPI. The value of ΔGexSCm was calculated by integration of area deviation (ΔA) over the surface pressure (Eq. (7)). The molar ratios of Chol in the SCm, rChol, are 0 (filled inverted triangle), 0.3 (filled square), 0.6 (filled circle), 0.9 (filled diamond) and 1.0 (filled triangle).

Mentions: Dependence of ΔGexSCm on XPI with the rChol value fixed at 0, 0.3, 0.6, 0.9 and 1.0 is shown in Figure 4. The energetic analysis supported the results of the mean molecular area analysis in PI/SM and PI/Chol monolayers that PI interacted repulsively with SM and attractively with Chol over the whole range of XPI. The maximum of ΔGex in the SM/PI mixed monolayers (rChol =0) seemed to lie at XPI = 0.1∼0.2 rather than XPI = 0.5, where the number of the pair of PI and SM would be the largest if they mixed homogeneously. Therefore, the PI and SM molecules may segregate weakly at higher XPI. In the PI/SCm ternary mixtures, ΔGexSCm changed from positive to negative as rChol increased. The value of ΔGexSCm was nearly equal to zero at rChol∼0.6. Thus, the PI/SCm monlayers at 30 mN/m became thermodynamically more stable as rChol increased.


Intermolecular interaction of phosphatidylinositol with the lipid raft molecules sphingomyelin and cholesterol
Excess mixing energy of PI and SM/Chol mixtures (SCm), ΔGexSCm, as a function of XPI. The value of ΔGexSCm was calculated by integration of area deviation (ΔA) over the surface pressure (Eq. (7)). The molar ratios of Chol in the SCm, rChol, are 0 (filled inverted triangle), 0.3 (filled square), 0.6 (filled circle), 0.9 (filled diamond) and 1.0 (filled triangle).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5036605&req=5

f4-4_1: Excess mixing energy of PI and SM/Chol mixtures (SCm), ΔGexSCm, as a function of XPI. The value of ΔGexSCm was calculated by integration of area deviation (ΔA) over the surface pressure (Eq. (7)). The molar ratios of Chol in the SCm, rChol, are 0 (filled inverted triangle), 0.3 (filled square), 0.6 (filled circle), 0.9 (filled diamond) and 1.0 (filled triangle).
Mentions: Dependence of ΔGexSCm on XPI with the rChol value fixed at 0, 0.3, 0.6, 0.9 and 1.0 is shown in Figure 4. The energetic analysis supported the results of the mean molecular area analysis in PI/SM and PI/Chol monolayers that PI interacted repulsively with SM and attractively with Chol over the whole range of XPI. The maximum of ΔGex in the SM/PI mixed monolayers (rChol =0) seemed to lie at XPI = 0.1∼0.2 rather than XPI = 0.5, where the number of the pair of PI and SM would be the largest if they mixed homogeneously. Therefore, the PI and SM molecules may segregate weakly at higher XPI. In the PI/SCm ternary mixtures, ΔGexSCm changed from positive to negative as rChol increased. The value of ΔGexSCm was nearly equal to zero at rChol∼0.6. Thus, the PI/SCm monlayers at 30 mN/m became thermodynamically more stable as rChol increased.

View Article: PubMed Central - PubMed

ABSTRACT

Diacylphosphatidylinositol (PI) is the starting reactant in the process of phosphatidylinositide-related signal transduction mediated through the lipid raft domain. We investigated intermolecular interactions of PI with major raft components, sphingomyelin (SM) and cholesterol (Chol), using surface pressure–molecular area (π–A) isotherm measurements. The classical mean molecular area versus composition plot showed that the measured mean molecular areas are smaller in PI/Chol mixed monolayers and larger in PI/SM mixed monolayers than those calculated on the basis of the ideal additivity. These results indicate that PI interacts attractively with Chol and repulsively with SM. In addition, we energetically evaluated the interaction of PI with SM/Chol mixtures and found that the mixing energy of PI/SM/Chol ternary monolayers decreased as the molar ratio of Chol to SM increased. In order to quantitatively analyze the distribution of PI we calculated the chemical potentials of mixing of PI into the SM/Chol mixed monolayer and into the dioleoylphosphatidylcholine (DOPC) monolayer, which was used as a model for the fluid matrix, on the basis of partial molecular area analysis. Analysis using the chemical potential of mixing of PI suggested that partition of PI molecules between these two monolayers can be changed by a factor of about 1.7 in response to change in Chol molar fraction in the SM/Chol mixed monolayer from 0.3 to 0.6 when the concentration of PI in the DOPC monolayer is kept constant at 7 mol%.

No MeSH data available.