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Multimodal near-infrared-emitting PluS Silica nanoparticles with fluorescent, photoacoustic, and photothermal capabilities

View Article: PubMed Central - PubMed

ABSTRACT

Purpose: The aim of the present study was to develop nanoprobes with theranostic features, including – at the same time – photoacoustic, near-infrared (NIR) optical imaging, and photothermal properties, in a versatile and stable core–shell silica-polyethylene glycol (PEG) nanoparticle architecture.

Materials and methods: We synthesized core–shell silica-PEG nanoparticles by a one-pot direct micelles approach. Fluorescence emission and photoacoustic and photothermal properties were obtained at the same time by appropriate doping with triethoxysilane-derivatized cyanine 5.5 (Cy5.5) and cyanine 7 (Cy7) dyes. The performances of these nanoprobes were measured in vitro, using nanoparticle suspensions in phosphate-buffered saline and blood, dedicated phantoms, and after incubation with MDA-MB-231 cells.

Results: We obtained core–shell silica-PEG nanoparticles endowed with very high colloidal stability in water and in biological environment, with absorption and fluorescence emission in the NIR field. The presence of Cy5.5 and Cy7 dyes made it possible to reach a more reproducible and higher doping regime, producing fluorescence emission at a single excitation wavelength in two different channels, owing to the energy transfer processes within the nanoparticle. The nanoarchitecture and the presence of both Cy5.5 and Cy7 dyes provided a favorable agreement between fluorescence emission and quenching, to achieve optical imaging and photoacoustic and photothermal properties.

Conclusion: We obtained rationally designed nanoparticles with outstanding stability in biological environment. At appropriate doping regimes, the presence of Cy5.5 and Cy7 dyes allowed us to tune fluorescence emission in the NIR for optical imaging and to exploit quenching processes for photoacoustic and photothermal capabilities. These nanostructures are promising in vivo theranostic tools for the near future.

No MeSH data available.


Related in: MedlinePlus

Comparison of fluorescence properties of NIR-PluS NP preparations.Notes: (A) UV-Vis spectra. (B) Equimolar aliquots of the NIR-PluS NP preparatives were placed on a paper substrate before analysis of the fluorescence with a time-domain fluorescence imager. Data are expressed as mean ± SD of three independent experiments.Abbreviations: NIR, near infrared; NIR-PluS NPs, NIR-emitting pluronic-silica nanoparticles; NP, nanoparticle; NC, normalized counts; Cy5.5, cyanine 5.5; Cy7, cyanine 7.
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f2-ijn-11-4865: Comparison of fluorescence properties of NIR-PluS NP preparations.Notes: (A) UV-Vis spectra. (B) Equimolar aliquots of the NIR-PluS NP preparatives were placed on a paper substrate before analysis of the fluorescence with a time-domain fluorescence imager. Data are expressed as mean ± SD of three independent experiments.Abbreviations: NIR, near infrared; NIR-PluS NPs, NIR-emitting pluronic-silica nanoparticles; NP, nanoparticle; NC, normalized counts; Cy5.5, cyanine 5.5; Cy7, cyanine 7.

Mentions: NIR cyanine 5.5 (Cy5.5) and cyanine 7 (Cy7) dyes (Figures 1 and S17–S42) were simultaneously covalently embedded into the PluS NPs to develop a bright NIR emitting NP material free of dye leaching with both absorption and emission in the NIR range. In particular, by varying the doping ratio of the two dyes (Table 1), absorption and emission profiles could be tuned to have the NIR-PluS NPs exhibiting multiple emission wavelengths by single wavelength excitation (Figures 2A and S43–S49).


Multimodal near-infrared-emitting PluS Silica nanoparticles with fluorescent, photoacoustic, and photothermal capabilities
Comparison of fluorescence properties of NIR-PluS NP preparations.Notes: (A) UV-Vis spectra. (B) Equimolar aliquots of the NIR-PluS NP preparatives were placed on a paper substrate before analysis of the fluorescence with a time-domain fluorescence imager. Data are expressed as mean ± SD of three independent experiments.Abbreviations: NIR, near infrared; NIR-PluS NPs, NIR-emitting pluronic-silica nanoparticles; NP, nanoparticle; NC, normalized counts; Cy5.5, cyanine 5.5; Cy7, cyanine 7.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5036595&req=5

f2-ijn-11-4865: Comparison of fluorescence properties of NIR-PluS NP preparations.Notes: (A) UV-Vis spectra. (B) Equimolar aliquots of the NIR-PluS NP preparatives were placed on a paper substrate before analysis of the fluorescence with a time-domain fluorescence imager. Data are expressed as mean ± SD of three independent experiments.Abbreviations: NIR, near infrared; NIR-PluS NPs, NIR-emitting pluronic-silica nanoparticles; NP, nanoparticle; NC, normalized counts; Cy5.5, cyanine 5.5; Cy7, cyanine 7.
Mentions: NIR cyanine 5.5 (Cy5.5) and cyanine 7 (Cy7) dyes (Figures 1 and S17–S42) were simultaneously covalently embedded into the PluS NPs to develop a bright NIR emitting NP material free of dye leaching with both absorption and emission in the NIR range. In particular, by varying the doping ratio of the two dyes (Table 1), absorption and emission profiles could be tuned to have the NIR-PluS NPs exhibiting multiple emission wavelengths by single wavelength excitation (Figures 2A and S43–S49).

View Article: PubMed Central - PubMed

ABSTRACT

Purpose: The aim of the present study was to develop nanoprobes with theranostic features, including – at the same time – photoacoustic, near-infrared (NIR) optical imaging, and photothermal properties, in a versatile and stable core–shell silica-polyethylene glycol (PEG) nanoparticle architecture.

Materials and methods: We synthesized core–shell silica-PEG nanoparticles by a one-pot direct micelles approach. Fluorescence emission and photoacoustic and photothermal properties were obtained at the same time by appropriate doping with triethoxysilane-derivatized cyanine 5.5 (Cy5.5) and cyanine 7 (Cy7) dyes. The performances of these nanoprobes were measured in vitro, using nanoparticle suspensions in phosphate-buffered saline and blood, dedicated phantoms, and after incubation with MDA-MB-231 cells.

Results: We obtained core–shell silica-PEG nanoparticles endowed with very high colloidal stability in water and in biological environment, with absorption and fluorescence emission in the NIR field. The presence of Cy5.5 and Cy7 dyes made it possible to reach a more reproducible and higher doping regime, producing fluorescence emission at a single excitation wavelength in two different channels, owing to the energy transfer processes within the nanoparticle. The nanoarchitecture and the presence of both Cy5.5 and Cy7 dyes provided a favorable agreement between fluorescence emission and quenching, to achieve optical imaging and photoacoustic and photothermal properties.

Conclusion: We obtained rationally designed nanoparticles with outstanding stability in biological environment. At appropriate doping regimes, the presence of Cy5.5 and Cy7 dyes allowed us to tune fluorescence emission in the NIR for optical imaging and to exploit quenching processes for photoacoustic and photothermal capabilities. These nanostructures are promising in vivo theranostic tools for the near future.

No MeSH data available.


Related in: MedlinePlus