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Controlled release of TGF-beta 1 from RADA self-assembling peptide hydrogel scaffolds

View Article: PubMed Central - PubMed

ABSTRACT

Bioactive mediators, cytokines, and chemokines have an important role in regulating and optimizing the synergistic action of materials, cells, and cellular microenvironments for tissue engineering. RADA self-assembling peptide hydrogels have been proved to have an excellent ability to promote cell proliferation, wound healing, tissue repair, and drug delivery. Here, we report that D-RADA16 and L-RADA16-RGD self-assembling peptides can form stable second structure and hydrogel scaffolds, affording the slow release of growth factor (transforming growth factor cytokine-beta 1 [TGF-beta 1]). In vitro tests demonstrated that the plateau release amount can be obtained till 72 hours. Moreover, L-RADA16, D-RADA16, and L-RADA16-RGD self-assembling peptide hydrogels containing TGF-beta 1 were used for 3D cell culture of bone mesenchymal stem cells of rats for 2 weeks. The results revealed that these three RADA16 peptide hydrogels had a significantly favorable influence on proliferation of bone mesenchymal stem cells and hold some promise in slow and sustained release of growth factor.

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Release profiles for TGF-beta 1 through three types of self-assembling peptide hydrogels 3D cells coculture (data points represent the average of three samples).Abbreviations: h, hours; TGF, transforming growth factor; 3D, three-dimensional.
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f5-dddt-10-3043: Release profiles for TGF-beta 1 through three types of self-assembling peptide hydrogels 3D cells coculture (data points represent the average of three samples).Abbreviations: h, hours; TGF, transforming growth factor; 3D, three-dimensional.

Mentions: The release experiments were observed for 14 days. The release profiles of TGF-beta 1 from the RADA peptide hydrogels are shown in Figures 4 and 5. Results revealed that ~18% (Figure 4) and 31% (Figure 5) of incorporated TGF were released from 1% w/v L-RADA16-RGD hydrogel within the first 24 hours. In contrast, only 17% (Figure 4) and 20% (Figure 5) of TGF-beta 1 were released from the D-RADA16 hydrogel within 24 hours. These results demonstrated that protein release rate in different types of peptide hydrogel was dissimilar. Compared with the D-RADA16 hydrogel, the initial burst effect of the L-RADA16-RGD hydrogel was higher.


Controlled release of TGF-beta 1 from RADA self-assembling peptide hydrogel scaffolds
Release profiles for TGF-beta 1 through three types of self-assembling peptide hydrogels 3D cells coculture (data points represent the average of three samples).Abbreviations: h, hours; TGF, transforming growth factor; 3D, three-dimensional.
© Copyright Policy
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5036568&req=5

f5-dddt-10-3043: Release profiles for TGF-beta 1 through three types of self-assembling peptide hydrogels 3D cells coculture (data points represent the average of three samples).Abbreviations: h, hours; TGF, transforming growth factor; 3D, three-dimensional.
Mentions: The release experiments were observed for 14 days. The release profiles of TGF-beta 1 from the RADA peptide hydrogels are shown in Figures 4 and 5. Results revealed that ~18% (Figure 4) and 31% (Figure 5) of incorporated TGF were released from 1% w/v L-RADA16-RGD hydrogel within the first 24 hours. In contrast, only 17% (Figure 4) and 20% (Figure 5) of TGF-beta 1 were released from the D-RADA16 hydrogel within 24 hours. These results demonstrated that protein release rate in different types of peptide hydrogel was dissimilar. Compared with the D-RADA16 hydrogel, the initial burst effect of the L-RADA16-RGD hydrogel was higher.

View Article: PubMed Central - PubMed

ABSTRACT

Bioactive mediators, cytokines, and chemokines have an important role in regulating and optimizing the synergistic action of materials, cells, and cellular microenvironments for tissue engineering. RADA self-assembling peptide hydrogels have been proved to have an excellent ability to promote cell proliferation, wound healing, tissue repair, and drug delivery. Here, we report that D-RADA16 and L-RADA16-RGD self-assembling peptides can form stable second structure and hydrogel scaffolds, affording the slow release of growth factor (transforming growth factor cytokine-beta 1 [TGF-beta 1]). In vitro tests demonstrated that the plateau release amount can be obtained till 72 hours. Moreover, L-RADA16, D-RADA16, and L-RADA16-RGD self-assembling peptide hydrogels containing TGF-beta 1 were used for 3D cell culture of bone mesenchymal stem cells of rats for 2 weeks. The results revealed that these three RADA16 peptide hydrogels had a significantly favorable influence on proliferation of bone mesenchymal stem cells and hold some promise in slow and sustained release of growth factor.

No MeSH data available.


Related in: MedlinePlus