Limits...
Controlled release of TGF-beta 1 from RADA self-assembling peptide hydrogel scaffolds

View Article: PubMed Central - PubMed

ABSTRACT

Bioactive mediators, cytokines, and chemokines have an important role in regulating and optimizing the synergistic action of materials, cells, and cellular microenvironments for tissue engineering. RADA self-assembling peptide hydrogels have been proved to have an excellent ability to promote cell proliferation, wound healing, tissue repair, and drug delivery. Here, we report that D-RADA16 and L-RADA16-RGD self-assembling peptides can form stable second structure and hydrogel scaffolds, affording the slow release of growth factor (transforming growth factor cytokine-beta 1 [TGF-beta 1]). In vitro tests demonstrated that the plateau release amount can be obtained till 72 hours. Moreover, L-RADA16, D-RADA16, and L-RADA16-RGD self-assembling peptide hydrogels containing TGF-beta 1 were used for 3D cell culture of bone mesenchymal stem cells of rats for 2 weeks. The results revealed that these three RADA16 peptide hydrogels had a significantly favorable influence on proliferation of bone mesenchymal stem cells and hold some promise in slow and sustained release of growth factor.

No MeSH data available.


Rheological assays: 2.5, 5.0 mg/mL peptide hydrogels in PBS (pH 7.4), 25°C.Notes: (A) The rheological properties of 2.5 and 5.0 mg/mL L-RADA16 hydrogels in 10.0 mg/mL PBS (pH 7.4) at 25°C. (B) The rheological properties of 2.5 and 5.0 mg/mL D-RADA16 hydrogels in 10.0 mg/mL PBS (pH 7.4) at 25°C.Abbreviation: PBS, phosphate-buffered saline.
© Copyright Policy
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC5036568&req=5

f3-dddt-10-3043: Rheological assays: 2.5, 5.0 mg/mL peptide hydrogels in PBS (pH 7.4), 25°C.Notes: (A) The rheological properties of 2.5 and 5.0 mg/mL L-RADA16 hydrogels in 10.0 mg/mL PBS (pH 7.4) at 25°C. (B) The rheological properties of 2.5 and 5.0 mg/mL D-RADA16 hydrogels in 10.0 mg/mL PBS (pH 7.4) at 25°C.Abbreviation: PBS, phosphate-buffered saline.

Mentions: These peptides can self-assemble into nanofibers and further hydrogelation occurs. The earliest few minutes of hydrogelation are crucial to keep cells in the truly 3D microenvironment. However, gelation nanomechanical properties in the cell media are poorly clear, especially in the initial phase. We chose typical ions (Na+/K+) in the cell media to investigate the gelation nanomechanical properties. Rheological experiments measure a storage modulus (G′) and a loss modulus (G″) by evaluating elastic response (rigidity) and viscous response of material, respectively, by varying frequencies of applied oscillatory stress. Upon comparing the rheological property of D-RADA16 and L-RADA16 (1 mg/mL) self-assembly in water or salt solution (0.01 M PBS, Na+/K+), D-RADA16 peptide is more sensitive to ions than L-RADA16, and the frequency G′ was greater than G″ (Figure 3A). High concentration of peptides was revealed to result in higher frequency G′ (20.0 mg/mL), both D- and L-RADA16 >12 Pa (Figure 3A and B), and D-form peptide showed a little bit lower frequency G′ (Figure 3B). High concentration of D-RADA16 and L-RADA16 demonstrated similar mechanical properties and Na+/K+ can affect them immediately. A reasonable explanation is that Na+/K+ can induce the peptides to self-assemble into short nanofibers rapidly. Our result is useful in a process of 3D cell culture.


Controlled release of TGF-beta 1 from RADA self-assembling peptide hydrogel scaffolds
Rheological assays: 2.5, 5.0 mg/mL peptide hydrogels in PBS (pH 7.4), 25°C.Notes: (A) The rheological properties of 2.5 and 5.0 mg/mL L-RADA16 hydrogels in 10.0 mg/mL PBS (pH 7.4) at 25°C. (B) The rheological properties of 2.5 and 5.0 mg/mL D-RADA16 hydrogels in 10.0 mg/mL PBS (pH 7.4) at 25°C.Abbreviation: PBS, phosphate-buffered saline.
© Copyright Policy
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5036568&req=5

f3-dddt-10-3043: Rheological assays: 2.5, 5.0 mg/mL peptide hydrogels in PBS (pH 7.4), 25°C.Notes: (A) The rheological properties of 2.5 and 5.0 mg/mL L-RADA16 hydrogels in 10.0 mg/mL PBS (pH 7.4) at 25°C. (B) The rheological properties of 2.5 and 5.0 mg/mL D-RADA16 hydrogels in 10.0 mg/mL PBS (pH 7.4) at 25°C.Abbreviation: PBS, phosphate-buffered saline.
Mentions: These peptides can self-assemble into nanofibers and further hydrogelation occurs. The earliest few minutes of hydrogelation are crucial to keep cells in the truly 3D microenvironment. However, gelation nanomechanical properties in the cell media are poorly clear, especially in the initial phase. We chose typical ions (Na+/K+) in the cell media to investigate the gelation nanomechanical properties. Rheological experiments measure a storage modulus (G′) and a loss modulus (G″) by evaluating elastic response (rigidity) and viscous response of material, respectively, by varying frequencies of applied oscillatory stress. Upon comparing the rheological property of D-RADA16 and L-RADA16 (1 mg/mL) self-assembly in water or salt solution (0.01 M PBS, Na+/K+), D-RADA16 peptide is more sensitive to ions than L-RADA16, and the frequency G′ was greater than G″ (Figure 3A). High concentration of peptides was revealed to result in higher frequency G′ (20.0 mg/mL), both D- and L-RADA16 >12 Pa (Figure 3A and B), and D-form peptide showed a little bit lower frequency G′ (Figure 3B). High concentration of D-RADA16 and L-RADA16 demonstrated similar mechanical properties and Na+/K+ can affect them immediately. A reasonable explanation is that Na+/K+ can induce the peptides to self-assemble into short nanofibers rapidly. Our result is useful in a process of 3D cell culture.

View Article: PubMed Central - PubMed

ABSTRACT

Bioactive mediators, cytokines, and chemokines have an important role in regulating and optimizing the synergistic action of materials, cells, and cellular microenvironments for tissue engineering. RADA self-assembling peptide hydrogels have been proved to have an excellent ability to promote cell proliferation, wound healing, tissue repair, and drug delivery. Here, we report that D-RADA16 and L-RADA16-RGD self-assembling peptides can form stable second structure and hydrogel scaffolds, affording the slow release of growth factor (transforming growth factor cytokine-beta 1 [TGF-beta 1]). In vitro tests demonstrated that the plateau release amount can be obtained till 72 hours. Moreover, L-RADA16, D-RADA16, and L-RADA16-RGD self-assembling peptide hydrogels containing TGF-beta 1 were used for 3D cell culture of bone mesenchymal stem cells of rats for 2 weeks. The results revealed that these three RADA16 peptide hydrogels had a significantly favorable influence on proliferation of bone mesenchymal stem cells and hold some promise in slow and sustained release of growth factor.

No MeSH data available.