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Controlled release of TGF-beta 1 from RADA self-assembling peptide hydrogel scaffolds

View Article: PubMed Central - PubMed

ABSTRACT

Bioactive mediators, cytokines, and chemokines have an important role in regulating and optimizing the synergistic action of materials, cells, and cellular microenvironments for tissue engineering. RADA self-assembling peptide hydrogels have been proved to have an excellent ability to promote cell proliferation, wound healing, tissue repair, and drug delivery. Here, we report that D-RADA16 and L-RADA16-RGD self-assembling peptides can form stable second structure and hydrogel scaffolds, affording the slow release of growth factor (transforming growth factor cytokine-beta 1 [TGF-beta 1]). In vitro tests demonstrated that the plateau release amount can be obtained till 72 hours. Moreover, L-RADA16, D-RADA16, and L-RADA16-RGD self-assembling peptide hydrogels containing TGF-beta 1 were used for 3D cell culture of bone mesenchymal stem cells of rats for 2 weeks. The results revealed that these three RADA16 peptide hydrogels had a significantly favorable influence on proliferation of bone mesenchymal stem cells and hold some promise in slow and sustained release of growth factor.

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TEM images of microstructure of (A) L-RADA16, (B) D-RADA16, and (C) L-RADA16-RGD (200 nm).Note: Magnification: (A) ×75,000; (B) ×60,000; and (C) ×75,000.Abbreviation: TEM, transmission electron microscopy.
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f2-dddt-10-3043: TEM images of microstructure of (A) L-RADA16, (B) D-RADA16, and (C) L-RADA16-RGD (200 nm).Note: Magnification: (A) ×75,000; (B) ×60,000; and (C) ×75,000.Abbreviation: TEM, transmission electron microscopy.

Mentions: TEM was applied to observe the microstructure of the RADA16 peptide hydrogels. TEM images showed that the RADA16 peptides were able to self-assemble into interwoven nanofibers at 25°C (Figure 2). These nanofibers were ~10 nm in diameter and formed pores <200 nm, whereas the diameter of nanofibers of L-RADA16-RGD was wider than that of the other peptides. The physical network and these structures coincided with the mechanism of interwoven formation in these peptide hydrogels.


Controlled release of TGF-beta 1 from RADA self-assembling peptide hydrogel scaffolds
TEM images of microstructure of (A) L-RADA16, (B) D-RADA16, and (C) L-RADA16-RGD (200 nm).Note: Magnification: (A) ×75,000; (B) ×60,000; and (C) ×75,000.Abbreviation: TEM, transmission electron microscopy.
© Copyright Policy
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5036568&req=5

f2-dddt-10-3043: TEM images of microstructure of (A) L-RADA16, (B) D-RADA16, and (C) L-RADA16-RGD (200 nm).Note: Magnification: (A) ×75,000; (B) ×60,000; and (C) ×75,000.Abbreviation: TEM, transmission electron microscopy.
Mentions: TEM was applied to observe the microstructure of the RADA16 peptide hydrogels. TEM images showed that the RADA16 peptides were able to self-assemble into interwoven nanofibers at 25°C (Figure 2). These nanofibers were ~10 nm in diameter and formed pores <200 nm, whereas the diameter of nanofibers of L-RADA16-RGD was wider than that of the other peptides. The physical network and these structures coincided with the mechanism of interwoven formation in these peptide hydrogels.

View Article: PubMed Central - PubMed

ABSTRACT

Bioactive mediators, cytokines, and chemokines have an important role in regulating and optimizing the synergistic action of materials, cells, and cellular microenvironments for tissue engineering. RADA self-assembling peptide hydrogels have been proved to have an excellent ability to promote cell proliferation, wound healing, tissue repair, and drug delivery. Here, we report that D-RADA16 and L-RADA16-RGD self-assembling peptides can form stable second structure and hydrogel scaffolds, affording the slow release of growth factor (transforming growth factor cytokine-beta 1 [TGF-beta 1]). In vitro tests demonstrated that the plateau release amount can be obtained till 72 hours. Moreover, L-RADA16, D-RADA16, and L-RADA16-RGD self-assembling peptide hydrogels containing TGF-beta 1 were used for 3D cell culture of bone mesenchymal stem cells of rats for 2 weeks. The results revealed that these three RADA16 peptide hydrogels had a significantly favorable influence on proliferation of bone mesenchymal stem cells and hold some promise in slow and sustained release of growth factor.

No MeSH data available.


Related in: MedlinePlus