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Controlled release of TGF-beta 1 from RADA self-assembling peptide hydrogel scaffolds

View Article: PubMed Central - PubMed

ABSTRACT

Bioactive mediators, cytokines, and chemokines have an important role in regulating and optimizing the synergistic action of materials, cells, and cellular microenvironments for tissue engineering. RADA self-assembling peptide hydrogels have been proved to have an excellent ability to promote cell proliferation, wound healing, tissue repair, and drug delivery. Here, we report that D-RADA16 and L-RADA16-RGD self-assembling peptides can form stable second structure and hydrogel scaffolds, affording the slow release of growth factor (transforming growth factor cytokine-beta 1 [TGF-beta 1]). In vitro tests demonstrated that the plateau release amount can be obtained till 72 hours. Moreover, L-RADA16, D-RADA16, and L-RADA16-RGD self-assembling peptide hydrogels containing TGF-beta 1 were used for 3D cell culture of bone mesenchymal stem cells of rats for 2 weeks. The results revealed that these three RADA16 peptide hydrogels had a significantly favorable influence on proliferation of bone mesenchymal stem cells and hold some promise in slow and sustained release of growth factor.

No MeSH data available.


Circular dichroism spectra of the chiral peptides.Notes: Peptide L-RADA16-RGD of 50 μM has stable secondary structures in 20 mM NaCl at 25°C, 193.5 nm (13.5827), 193 nm (12.4508), and 198 nm (−15.8484).Abbreviation: deg, degree.
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f1-dddt-10-3043: Circular dichroism spectra of the chiral peptides.Notes: Peptide L-RADA16-RGD of 50 μM has stable secondary structures in 20 mM NaCl at 25°C, 193.5 nm (13.5827), 193 nm (12.4508), and 198 nm (−15.8484).Abbreviation: deg, degree.

Mentions: The β-sheet structure of L-RADA16-RGD is shown in Figure 1, indicating that L-RADA16-RGD peptide could produce stable secondary structures in 20 mM NaCl at 25°C. This result indicates that the 3D cell culture should be handled quickly at room temperature.


Controlled release of TGF-beta 1 from RADA self-assembling peptide hydrogel scaffolds
Circular dichroism spectra of the chiral peptides.Notes: Peptide L-RADA16-RGD of 50 μM has stable secondary structures in 20 mM NaCl at 25°C, 193.5 nm (13.5827), 193 nm (12.4508), and 198 nm (−15.8484).Abbreviation: deg, degree.
© Copyright Policy
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5036568&req=5

f1-dddt-10-3043: Circular dichroism spectra of the chiral peptides.Notes: Peptide L-RADA16-RGD of 50 μM has stable secondary structures in 20 mM NaCl at 25°C, 193.5 nm (13.5827), 193 nm (12.4508), and 198 nm (−15.8484).Abbreviation: deg, degree.
Mentions: The β-sheet structure of L-RADA16-RGD is shown in Figure 1, indicating that L-RADA16-RGD peptide could produce stable secondary structures in 20 mM NaCl at 25°C. This result indicates that the 3D cell culture should be handled quickly at room temperature.

View Article: PubMed Central - PubMed

ABSTRACT

Bioactive mediators, cytokines, and chemokines have an important role in regulating and optimizing the synergistic action of materials, cells, and cellular microenvironments for tissue engineering. RADA self-assembling peptide hydrogels have been proved to have an excellent ability to promote cell proliferation, wound healing, tissue repair, and drug delivery. Here, we report that D-RADA16 and L-RADA16-RGD self-assembling peptides can form stable second structure and hydrogel scaffolds, affording the slow release of growth factor (transforming growth factor cytokine-beta 1 [TGF-beta 1]). In vitro tests demonstrated that the plateau release amount can be obtained till 72 hours. Moreover, L-RADA16, D-RADA16, and L-RADA16-RGD self-assembling peptide hydrogels containing TGF-beta 1 were used for 3D cell culture of bone mesenchymal stem cells of rats for 2 weeks. The results revealed that these three RADA16 peptide hydrogels had a significantly favorable influence on proliferation of bone mesenchymal stem cells and hold some promise in slow and sustained release of growth factor.

No MeSH data available.