Limits...
Shape-memory surfaces for cell mechanobiology

View Article: PubMed Central - PubMed

ABSTRACT

Shape-memory polymers (SMPs) are a new class of smart materials, which have the capability to change from a temporary shape ‘A’ to a memorized permanent shape ‘B’ upon application of an external stimulus. In recent years, SMPs have attracted much attention from basic and fundamental research to industrial and practical applications due to the cheap and efficient alternative to well-known metallic shape-memory alloys. Since the shape-memory effect in SMPs is not related to a specific material property of single polymers, the control of nanoarchitecture of polymer networks is particularly important for the smart functions of SMPs. Such nanoarchitectonic approaches have enabled us to further create shape-memory surfaces (SMSs) with tunable surface topography at nano scale. The present review aims to bring together the exciting design of SMSs and the ever-expanding range of their uses as tools to control cell functions. The goal for these endeavors is to mimic the surrounding mechanical cues of extracellular environments which have been considered as critical parameters in cell fate determination. The untapped potential of SMSs makes them one of the most exciting interfaces of materials science and cell mechanobiology.

No MeSH data available.


YAP/TAZ nuclear localization is sensitive to dynamic changes in substrate nanopattern in cardiac progenitor cells. PCL-based thermo-responsive substrates having shape-memory effect were produced to display a temporary nanopattern at 32 °C. The surfaces could be induced to a thermal transition after switching the temperature to 37 °C to acquire a permanent flat surface. When shape-memory transition is activated, cells are displaced from the surface and encounter a dramatic but transient switch in YAP/TAZ nuclear expression, which is restored after 180 min.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC5036502&req=5

Figure 8: YAP/TAZ nuclear localization is sensitive to dynamic changes in substrate nanopattern in cardiac progenitor cells. PCL-based thermo-responsive substrates having shape-memory effect were produced to display a temporary nanopattern at 32 °C. The surfaces could be induced to a thermal transition after switching the temperature to 37 °C to acquire a permanent flat surface. When shape-memory transition is activated, cells are displaced from the surface and encounter a dramatic but transient switch in YAP/TAZ nuclear expression, which is restored after 180 min.

Mentions: These dynamic changes in nanotopography created on PCL substrate can also influence the intracellular localization of YAP/TAZ in cardiac progenitor cells [83]. YAP and TAZ are respectively Yes-associated protein YAP and WW domain-containing transcription regulator protein 1 which are the downstream effectors of the Hippo pathway. The YAP/TAZ subcellular localization, strictly regulated by cell–cell and cell–ECM interaction, has been correlated to cell mechanosensing. Following surface change from nanotopography (300 nm of line ridge, 500 nm of groove, 120 nm of height) to flat by switching temperature to 32–37 °C, a significant decrease in nuclear expression of YAP/TAZ could be detected after 90 min. However, the percentage of YAP nuclear positive cells returned to the original values after 180 min (figure 8). This result indicates that the YAP/TAZ transcriptional coactivators may act as intracellular mechanical rheostats mediating the effectors of mechanical dosing on stem cell plasticity by a persistent presence in the nucleus. In the future, these approaches will also enable not only unprecedented observations of time-dependent cell–substrate interactions without the need for invasive forces against intact adhered cells but also direct manipulation of cell function and fate.


Shape-memory surfaces for cell mechanobiology
YAP/TAZ nuclear localization is sensitive to dynamic changes in substrate nanopattern in cardiac progenitor cells. PCL-based thermo-responsive substrates having shape-memory effect were produced to display a temporary nanopattern at 32 °C. The surfaces could be induced to a thermal transition after switching the temperature to 37 °C to acquire a permanent flat surface. When shape-memory transition is activated, cells are displaced from the surface and encounter a dramatic but transient switch in YAP/TAZ nuclear expression, which is restored after 180 min.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5036502&req=5

Figure 8: YAP/TAZ nuclear localization is sensitive to dynamic changes in substrate nanopattern in cardiac progenitor cells. PCL-based thermo-responsive substrates having shape-memory effect were produced to display a temporary nanopattern at 32 °C. The surfaces could be induced to a thermal transition after switching the temperature to 37 °C to acquire a permanent flat surface. When shape-memory transition is activated, cells are displaced from the surface and encounter a dramatic but transient switch in YAP/TAZ nuclear expression, which is restored after 180 min.
Mentions: These dynamic changes in nanotopography created on PCL substrate can also influence the intracellular localization of YAP/TAZ in cardiac progenitor cells [83]. YAP and TAZ are respectively Yes-associated protein YAP and WW domain-containing transcription regulator protein 1 which are the downstream effectors of the Hippo pathway. The YAP/TAZ subcellular localization, strictly regulated by cell–cell and cell–ECM interaction, has been correlated to cell mechanosensing. Following surface change from nanotopography (300 nm of line ridge, 500 nm of groove, 120 nm of height) to flat by switching temperature to 32–37 °C, a significant decrease in nuclear expression of YAP/TAZ could be detected after 90 min. However, the percentage of YAP nuclear positive cells returned to the original values after 180 min (figure 8). This result indicates that the YAP/TAZ transcriptional coactivators may act as intracellular mechanical rheostats mediating the effectors of mechanical dosing on stem cell plasticity by a persistent presence in the nucleus. In the future, these approaches will also enable not only unprecedented observations of time-dependent cell–substrate interactions without the need for invasive forces against intact adhered cells but also direct manipulation of cell function and fate.

View Article: PubMed Central - PubMed

ABSTRACT

Shape-memory polymers (SMPs) are a new class of smart materials, which have the capability to change from a temporary shape ‘A’ to a memorized permanent shape ‘B’ upon application of an external stimulus. In recent years, SMPs have attracted much attention from basic and fundamental research to industrial and practical applications due to the cheap and efficient alternative to well-known metallic shape-memory alloys. Since the shape-memory effect in SMPs is not related to a specific material property of single polymers, the control of nanoarchitecture of polymer networks is particularly important for the smart functions of SMPs. Such nanoarchitectonic approaches have enabled us to further create shape-memory surfaces (SMSs) with tunable surface topography at nano scale. The present review aims to bring together the exciting design of SMSs and the ever-expanding range of their uses as tools to control cell functions. The goal for these endeavors is to mimic the surrounding mechanical cues of extracellular environments which have been considered as critical parameters in cell fate determination. The untapped potential of SMSs makes them one of the most exciting interfaces of materials science and cell mechanobiology.

No MeSH data available.