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Highly efficient in vitro biosynthesis of silver nanoparticles using Lysinibacillus sphaericus MR-1 and their characterization

View Article: PubMed Central - PubMed

ABSTRACT

Silver nanoparticles (AgNPs) have been widely used in diverse fields due to their superior properties. Currently the biosynthesis of AgNPs is in the limelight of modern nanotechnology because of its green properties. However, relatively low yield and inefficiency diminish the prospect of applying these biosynthesized AgNPs. In this work, a rapid mass AgNP biosynthesis method using the cell-free extract of a novel bacterial strain, Lysinibacillus sphaericus MR-1, which has been isolated from a chemical fertilizer plant, is reported. In addition, the optimum synthesis conditions of AgNPs were investigated. The optimum pH, temperature, dosage, and reaction time were 12, 70 °C, 20 mM AgNO3, and 75 min, respectively. Finally, AgNPs were characterized by optical absorption spectroscopy, zeta potential and size distribution analysis, x-ray diffraction, electron microscopy, and energy-dispersive x-ray spectroscopy. The results revealed that these biosynthesized AgNPs were bimolecular covered, stable, well-dispersed face centered cubic (fcc) spherical crystalline particles with diameters in the range 5–20 nm. The advantages of this approach are its simplicity, high efficiency, and eco-friendly and cost-effective features.

No MeSH data available.


Dynamic light scattering measurements for (a) particle size distribution analysis and (b) zeta potential measurements of AgNPs.
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Figure 6: Dynamic light scattering measurements for (a) particle size distribution analysis and (b) zeta potential measurements of AgNPs.

Mentions: The average size distribution of silver nanoparticles in colloidal solution was found to be 14.8 ± 1.2 nm (figure 6(a)). A negative zeta potential of about −41.6 ± 0.5 mV was observed in the current study that represents the ideal surface charge (figure 6(b)). A high absolute value of zeta potential denotes a high electrical charge on the surface of AgNPs, which can cause a strong repulsive force among the particles to prevent agglomeration and which thus might be responsible for the stable nature of the AgNPs.


Highly efficient in vitro biosynthesis of silver nanoparticles using Lysinibacillus sphaericus MR-1 and their characterization
Dynamic light scattering measurements for (a) particle size distribution analysis and (b) zeta potential measurements of AgNPs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5036493&req=5

Figure 6: Dynamic light scattering measurements for (a) particle size distribution analysis and (b) zeta potential measurements of AgNPs.
Mentions: The average size distribution of silver nanoparticles in colloidal solution was found to be 14.8 ± 1.2 nm (figure 6(a)). A negative zeta potential of about −41.6 ± 0.5 mV was observed in the current study that represents the ideal surface charge (figure 6(b)). A high absolute value of zeta potential denotes a high electrical charge on the surface of AgNPs, which can cause a strong repulsive force among the particles to prevent agglomeration and which thus might be responsible for the stable nature of the AgNPs.

View Article: PubMed Central - PubMed

ABSTRACT

Silver nanoparticles (AgNPs) have been widely used in diverse fields due to their superior properties. Currently the biosynthesis of AgNPs is in the limelight of modern nanotechnology because of its green properties. However, relatively low yield and inefficiency diminish the prospect of applying these biosynthesized AgNPs. In this work, a rapid mass AgNP biosynthesis method using the cell-free extract of a novel bacterial strain, Lysinibacillus sphaericus MR-1, which has been isolated from a chemical fertilizer plant, is reported. In addition, the optimum synthesis conditions of AgNPs were investigated. The optimum pH, temperature, dosage, and reaction time were 12, 70 °C, 20 mM AgNO3, and 75 min, respectively. Finally, AgNPs were characterized by optical absorption spectroscopy, zeta potential and size distribution analysis, x-ray diffraction, electron microscopy, and energy-dispersive x-ray spectroscopy. The results revealed that these biosynthesized AgNPs were bimolecular covered, stable, well-dispersed face centered cubic (fcc) spherical crystalline particles with diameters in the range 5–20 nm. The advantages of this approach are its simplicity, high efficiency, and eco-friendly and cost-effective features.

No MeSH data available.