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Highly efficient in vitro biosynthesis of silver nanoparticles using Lysinibacillus sphaericus MR-1 and their characterization

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ABSTRACT

Silver nanoparticles (AgNPs) have been widely used in diverse fields due to their superior properties. Currently the biosynthesis of AgNPs is in the limelight of modern nanotechnology because of its green properties. However, relatively low yield and inefficiency diminish the prospect of applying these biosynthesized AgNPs. In this work, a rapid mass AgNP biosynthesis method using the cell-free extract of a novel bacterial strain, Lysinibacillus sphaericus MR-1, which has been isolated from a chemical fertilizer plant, is reported. In addition, the optimum synthesis conditions of AgNPs were investigated. The optimum pH, temperature, dosage, and reaction time were 12, 70 °C, 20 mM AgNO3, and 75 min, respectively. Finally, AgNPs were characterized by optical absorption spectroscopy, zeta potential and size distribution analysis, x-ray diffraction, electron microscopy, and energy-dispersive x-ray spectroscopy. The results revealed that these biosynthesized AgNPs were bimolecular covered, stable, well-dispersed face centered cubic (fcc) spherical crystalline particles with diameters in the range 5–20 nm. The advantages of this approach are its simplicity, high efficiency, and eco-friendly and cost-effective features.

No MeSH data available.


Related in: MedlinePlus

(a) Effect of temperature on silver nanoparticle synthesis; inset shows a nearly linear relationship between maximum absorbance and a temperature in the range 20–70 °C. (b) Effect of pH on AgNP synthesis by L. sphaericus MR-1 cell-free extract, which showed that alkaline pH was a necessary condition of this biosynthesis procedure and pH 12 was an optimum condition; the inset shows the relationship between the maximum absorbance and pH in the range 6–13.
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Figure 3: (a) Effect of temperature on silver nanoparticle synthesis; inset shows a nearly linear relationship between maximum absorbance and a temperature in the range 20–70 °C. (b) Effect of pH on AgNP synthesis by L. sphaericus MR-1 cell-free extract, which showed that alkaline pH was a necessary condition of this biosynthesis procedure and pH 12 was an optimum condition; the inset shows the relationship between the maximum absorbance and pH in the range 6–13.

Mentions: While the effect of temperature on the synthesis of AgNPs was being investigated, it was found that the maximum absorbance of the reaction mixture had increased from 20 to 70 °C, whereas it had decreased from 70 to 90 °C. As shown in the inset of figure 3(a), a nearly linear relationship between the maximum absorbance and the temperature in the range 20–70 °C was presented. The result suggested that moderately elevated temperature accelerated the reduction process.


Highly efficient in vitro biosynthesis of silver nanoparticles using Lysinibacillus sphaericus MR-1 and their characterization
(a) Effect of temperature on silver nanoparticle synthesis; inset shows a nearly linear relationship between maximum absorbance and a temperature in the range 20–70 °C. (b) Effect of pH on AgNP synthesis by L. sphaericus MR-1 cell-free extract, which showed that alkaline pH was a necessary condition of this biosynthesis procedure and pH 12 was an optimum condition; the inset shows the relationship between the maximum absorbance and pH in the range 6–13.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5036493&req=5

Figure 3: (a) Effect of temperature on silver nanoparticle synthesis; inset shows a nearly linear relationship between maximum absorbance and a temperature in the range 20–70 °C. (b) Effect of pH on AgNP synthesis by L. sphaericus MR-1 cell-free extract, which showed that alkaline pH was a necessary condition of this biosynthesis procedure and pH 12 was an optimum condition; the inset shows the relationship between the maximum absorbance and pH in the range 6–13.
Mentions: While the effect of temperature on the synthesis of AgNPs was being investigated, it was found that the maximum absorbance of the reaction mixture had increased from 20 to 70 °C, whereas it had decreased from 70 to 90 °C. As shown in the inset of figure 3(a), a nearly linear relationship between the maximum absorbance and the temperature in the range 20–70 °C was presented. The result suggested that moderately elevated temperature accelerated the reduction process.

View Article: PubMed Central - PubMed

ABSTRACT

Silver nanoparticles (AgNPs) have been widely used in diverse fields due to their superior properties. Currently the biosynthesis of AgNPs is in the limelight of modern nanotechnology because of its green properties. However, relatively low yield and inefficiency diminish the prospect of applying these biosynthesized AgNPs. In this work, a rapid mass AgNP biosynthesis method using the cell-free extract of a novel bacterial strain, Lysinibacillus sphaericus MR-1, which has been isolated from a chemical fertilizer plant, is reported. In addition, the optimum synthesis conditions of AgNPs were investigated. The optimum pH, temperature, dosage, and reaction time were 12, 70 °C, 20 mM AgNO3, and 75 min, respectively. Finally, AgNPs were characterized by optical absorption spectroscopy, zeta potential and size distribution analysis, x-ray diffraction, electron microscopy, and energy-dispersive x-ray spectroscopy. The results revealed that these biosynthesized AgNPs were bimolecular covered, stable, well-dispersed face centered cubic (fcc) spherical crystalline particles with diameters in the range 5–20 nm. The advantages of this approach are its simplicity, high efficiency, and eco-friendly and cost-effective features.

No MeSH data available.


Related in: MedlinePlus