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Highly efficient in vitro biosynthesis of silver nanoparticles using Lysinibacillus sphaericus MR-1 and their characterization

View Article: PubMed Central - PubMed

ABSTRACT

Silver nanoparticles (AgNPs) have been widely used in diverse fields due to their superior properties. Currently the biosynthesis of AgNPs is in the limelight of modern nanotechnology because of its green properties. However, relatively low yield and inefficiency diminish the prospect of applying these biosynthesized AgNPs. In this work, a rapid mass AgNP biosynthesis method using the cell-free extract of a novel bacterial strain, Lysinibacillus sphaericus MR-1, which has been isolated from a chemical fertilizer plant, is reported. In addition, the optimum synthesis conditions of AgNPs were investigated. The optimum pH, temperature, dosage, and reaction time were 12, 70 °C, 20 mM AgNO3, and 75 min, respectively. Finally, AgNPs were characterized by optical absorption spectroscopy, zeta potential and size distribution analysis, x-ray diffraction, electron microscopy, and energy-dispersive x-ray spectroscopy. The results revealed that these biosynthesized AgNPs were bimolecular covered, stable, well-dispersed face centered cubic (fcc) spherical crystalline particles with diameters in the range 5–20 nm. The advantages of this approach are its simplicity, high efficiency, and eco-friendly and cost-effective features.

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(a) Color change of reaction mixture containing different constituents (1. YPK medium; 2. YPK medium with 5 mM of AgNO3; 3. L. sphaericus MR-1 (X11) cell-free extract; 4. L. sphaericus MR-1 (X11) cell-free extract with 5 mM AgNO3). After overnight incubation, only the L. sphaericus MR-1 (X11) cell-free extract with 5 mM of AgNO3 showed an obvious color change from clear pale yellow to dark brown, confirming the formation of silver nanoparticles. (b) UV–vis spectrum of the reaction mixture containing different constituents, only the L. sphaericus MR-1 (X11) cell-free extract after the overnight incubation of the 5 mM of AgNO3 showed a peak at 416 nm, further confirming the formation of silver nanoparticles.
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Figure 2: (a) Color change of reaction mixture containing different constituents (1. YPK medium; 2. YPK medium with 5 mM of AgNO3; 3. L. sphaericus MR-1 (X11) cell-free extract; 4. L. sphaericus MR-1 (X11) cell-free extract with 5 mM AgNO3). After overnight incubation, only the L. sphaericus MR-1 (X11) cell-free extract with 5 mM of AgNO3 showed an obvious color change from clear pale yellow to dark brown, confirming the formation of silver nanoparticles. (b) UV–vis spectrum of the reaction mixture containing different constituents, only the L. sphaericus MR-1 (X11) cell-free extract after the overnight incubation of the 5 mM of AgNO3 showed a peak at 416 nm, further confirming the formation of silver nanoparticles.

Mentions: In the experiment, the formation of AgNPs was visually confirmed by the color change of the mixture from pale yellow to dark brown. This change did not occur in the negative controls (figure 2(a)). The preliminary investigation of the biosynthesized AgNPs was carried out by UV–vis spectroscopic analysis. It can be readily observed that the characteristic surface plasmon resonance band peak of the mixture was at 416 nm (figure 2(b)), indicating the presence of AgNPs [19].


Highly efficient in vitro biosynthesis of silver nanoparticles using Lysinibacillus sphaericus MR-1 and their characterization
(a) Color change of reaction mixture containing different constituents (1. YPK medium; 2. YPK medium with 5 mM of AgNO3; 3. L. sphaericus MR-1 (X11) cell-free extract; 4. L. sphaericus MR-1 (X11) cell-free extract with 5 mM AgNO3). After overnight incubation, only the L. sphaericus MR-1 (X11) cell-free extract with 5 mM of AgNO3 showed an obvious color change from clear pale yellow to dark brown, confirming the formation of silver nanoparticles. (b) UV–vis spectrum of the reaction mixture containing different constituents, only the L. sphaericus MR-1 (X11) cell-free extract after the overnight incubation of the 5 mM of AgNO3 showed a peak at 416 nm, further confirming the formation of silver nanoparticles.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5036493&req=5

Figure 2: (a) Color change of reaction mixture containing different constituents (1. YPK medium; 2. YPK medium with 5 mM of AgNO3; 3. L. sphaericus MR-1 (X11) cell-free extract; 4. L. sphaericus MR-1 (X11) cell-free extract with 5 mM AgNO3). After overnight incubation, only the L. sphaericus MR-1 (X11) cell-free extract with 5 mM of AgNO3 showed an obvious color change from clear pale yellow to dark brown, confirming the formation of silver nanoparticles. (b) UV–vis spectrum of the reaction mixture containing different constituents, only the L. sphaericus MR-1 (X11) cell-free extract after the overnight incubation of the 5 mM of AgNO3 showed a peak at 416 nm, further confirming the formation of silver nanoparticles.
Mentions: In the experiment, the formation of AgNPs was visually confirmed by the color change of the mixture from pale yellow to dark brown. This change did not occur in the negative controls (figure 2(a)). The preliminary investigation of the biosynthesized AgNPs was carried out by UV–vis spectroscopic analysis. It can be readily observed that the characteristic surface plasmon resonance band peak of the mixture was at 416 nm (figure 2(b)), indicating the presence of AgNPs [19].

View Article: PubMed Central - PubMed

ABSTRACT

Silver nanoparticles (AgNPs) have been widely used in diverse fields due to their superior properties. Currently the biosynthesis of AgNPs is in the limelight of modern nanotechnology because of its green properties. However, relatively low yield and inefficiency diminish the prospect of applying these biosynthesized AgNPs. In this work, a rapid mass AgNP biosynthesis method using the cell-free extract of a novel bacterial strain, Lysinibacillus sphaericus MR-1, which has been isolated from a chemical fertilizer plant, is reported. In addition, the optimum synthesis conditions of AgNPs were investigated. The optimum pH, temperature, dosage, and reaction time were 12, 70 °C, 20 mM AgNO3, and 75 min, respectively. Finally, AgNPs were characterized by optical absorption spectroscopy, zeta potential and size distribution analysis, x-ray diffraction, electron microscopy, and energy-dispersive x-ray spectroscopy. The results revealed that these biosynthesized AgNPs were bimolecular covered, stable, well-dispersed face centered cubic (fcc) spherical crystalline particles with diameters in the range 5–20 nm. The advantages of this approach are its simplicity, high efficiency, and eco-friendly and cost-effective features.

No MeSH data available.


Related in: MedlinePlus