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Highly efficient in vitro biosynthesis of silver nanoparticles using Lysinibacillus sphaericus MR-1 and their characterization

View Article: PubMed Central - PubMed

ABSTRACT

Silver nanoparticles (AgNPs) have been widely used in diverse fields due to their superior properties. Currently the biosynthesis of AgNPs is in the limelight of modern nanotechnology because of its green properties. However, relatively low yield and inefficiency diminish the prospect of applying these biosynthesized AgNPs. In this work, a rapid mass AgNP biosynthesis method using the cell-free extract of a novel bacterial strain, Lysinibacillus sphaericus MR-1, which has been isolated from a chemical fertilizer plant, is reported. In addition, the optimum synthesis conditions of AgNPs were investigated. The optimum pH, temperature, dosage, and reaction time were 12, 70 °C, 20 mM AgNO3, and 75 min, respectively. Finally, AgNPs were characterized by optical absorption spectroscopy, zeta potential and size distribution analysis, x-ray diffraction, electron microscopy, and energy-dispersive x-ray spectroscopy. The results revealed that these biosynthesized AgNPs were bimolecular covered, stable, well-dispersed face centered cubic (fcc) spherical crystalline particles with diameters in the range 5–20 nm. The advantages of this approach are its simplicity, high efficiency, and eco-friendly and cost-effective features.

No MeSH data available.


(a) Pure pale-white colonies of L. sphaericus MR-1 isolated from soil by a nutrient agar plate. (b) Phylogenetic tree constructed through the neighbor-joining method based on 16 S rRNA gene nucleotide sequences of L. sphaericus MR-1 and a reference sequence retrieved from NCBI Gen Bank.
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Figure 1: (a) Pure pale-white colonies of L. sphaericus MR-1 isolated from soil by a nutrient agar plate. (b) Phylogenetic tree constructed through the neighbor-joining method based on 16 S rRNA gene nucleotide sequences of L. sphaericus MR-1 and a reference sequence retrieved from NCBI Gen Bank.

Mentions: The pure pale-white bacterial X11 (figure 1(a)) obtained on the nutrient agar plate was identified as L. sphaericus based on molecular analysis through 16 S rRNA sequencing studies (figure 1(b)) and was named L. sphaericus MR-1.


Highly efficient in vitro biosynthesis of silver nanoparticles using Lysinibacillus sphaericus MR-1 and their characterization
(a) Pure pale-white colonies of L. sphaericus MR-1 isolated from soil by a nutrient agar plate. (b) Phylogenetic tree constructed through the neighbor-joining method based on 16 S rRNA gene nucleotide sequences of L. sphaericus MR-1 and a reference sequence retrieved from NCBI Gen Bank.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5036493&req=5

Figure 1: (a) Pure pale-white colonies of L. sphaericus MR-1 isolated from soil by a nutrient agar plate. (b) Phylogenetic tree constructed through the neighbor-joining method based on 16 S rRNA gene nucleotide sequences of L. sphaericus MR-1 and a reference sequence retrieved from NCBI Gen Bank.
Mentions: The pure pale-white bacterial X11 (figure 1(a)) obtained on the nutrient agar plate was identified as L. sphaericus based on molecular analysis through 16 S rRNA sequencing studies (figure 1(b)) and was named L. sphaericus MR-1.

View Article: PubMed Central - PubMed

ABSTRACT

Silver nanoparticles (AgNPs) have been widely used in diverse fields due to their superior properties. Currently the biosynthesis of AgNPs is in the limelight of modern nanotechnology because of its green properties. However, relatively low yield and inefficiency diminish the prospect of applying these biosynthesized AgNPs. In this work, a rapid mass AgNP biosynthesis method using the cell-free extract of a novel bacterial strain, Lysinibacillus sphaericus MR-1, which has been isolated from a chemical fertilizer plant, is reported. In addition, the optimum synthesis conditions of AgNPs were investigated. The optimum pH, temperature, dosage, and reaction time were 12, 70 °C, 20 mM AgNO3, and 75 min, respectively. Finally, AgNPs were characterized by optical absorption spectroscopy, zeta potential and size distribution analysis, x-ray diffraction, electron microscopy, and energy-dispersive x-ray spectroscopy. The results revealed that these biosynthesized AgNPs were bimolecular covered, stable, well-dispersed face centered cubic (fcc) spherical crystalline particles with diameters in the range 5–20 nm. The advantages of this approach are its simplicity, high efficiency, and eco-friendly and cost-effective features.

No MeSH data available.