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Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery

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ABSTRACT

In this study, we determined the number of peripheral blood circulating tumor cells (CTCs) pre- and post-cryosurgery in patients with colorectal cancer liver metastasis as a reference for understanding the relevance of any changes to the efficacy of cryosurgery. CTC numbers and CTC-related gene expression were measured in the peripheral blood of 55 patients with colorectal liver metastasis at 1 day before and 7 and 30 d after cryoablation using magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS) combined with real-time quantitative PCR (RT-qPCR). The number of CTCs decreased significantly with postoperative time (P < 0.01). Delta cycle threshold values for the CTC-related genes CEA, Ep-CAM, CK18 and CK19 increased significantly after cryoablation. Furthermore, the expression of CEA, Ep-CAM, CK18 and CK19 decreased significantly with time after cryoablation (P < 0.01). RT-qPCR and FACS combined with MACS has significant diagnostic and prognostic value for evaluating the efficacy of cryosurgery in patients with advanced colorectal cancer.

No MeSH data available.


Changes in CTC marker expression at 1 day before and 7 and 30 d after cryoablation therapy (2−△△Ct method).
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f0004: Changes in CTC marker expression at 1 day before and 7 and 30 d after cryoablation therapy (2−△△Ct method).

Mentions: Tumor marker-related gene expression before and after cryotherapy was analyzed using the 2−△△Ct method. Compared with baseline, CEA expression showed fold changes of 0.50 ± 1.496 and 0.05 ± 0.094 at 7 and 30 d after cryotherapy; Ep-CAM showed fold changes of 0.27 ± 0.523 and 0.08 ± 0.203; CK18 showed fold changes of 0.20 ± 0.324 and 0.05 ± 0.113; and CK19 showed fold changes of 0.53 ± 1.849 and 0.20 ± 0.730. All of the measured 2−△△Ct values for the target genes were less than 1, demonstrating that the expression of CTC markers decreased with time after treatment (Fig. 4).Figure 4.


Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery
Changes in CTC marker expression at 1 day before and 7 and 30 d after cryoablation therapy (2−△△Ct method).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036405&req=5

f0004: Changes in CTC marker expression at 1 day before and 7 and 30 d after cryoablation therapy (2−△△Ct method).
Mentions: Tumor marker-related gene expression before and after cryotherapy was analyzed using the 2−△△Ct method. Compared with baseline, CEA expression showed fold changes of 0.50 ± 1.496 and 0.05 ± 0.094 at 7 and 30 d after cryotherapy; Ep-CAM showed fold changes of 0.27 ± 0.523 and 0.08 ± 0.203; CK18 showed fold changes of 0.20 ± 0.324 and 0.05 ± 0.113; and CK19 showed fold changes of 0.53 ± 1.849 and 0.20 ± 0.730. All of the measured 2−△△Ct values for the target genes were less than 1, demonstrating that the expression of CTC markers decreased with time after treatment (Fig. 4).Figure 4.

View Article: PubMed Central - PubMed

ABSTRACT

In this study, we determined the number of peripheral blood circulating tumor cells (CTCs) pre- and post-cryosurgery in patients with colorectal cancer liver metastasis as a reference for understanding the relevance of any changes to the efficacy of cryosurgery. CTC numbers and CTC-related gene expression were measured in the peripheral blood of 55 patients with colorectal liver metastasis at 1 day before and 7 and 30 d after cryoablation using magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS) combined with real-time quantitative PCR (RT-qPCR). The number of CTCs decreased significantly with postoperative time (P < 0.01). Delta cycle threshold values for the CTC-related genes CEA, Ep-CAM, CK18 and CK19 increased significantly after cryoablation. Furthermore, the expression of CEA, Ep-CAM, CK18 and CK19 decreased significantly with time after cryoablation (P < 0.01). RT-qPCR and FACS combined with MACS has significant diagnostic and prognostic value for evaluating the efficacy of cryosurgery in patients with advanced colorectal cancer.

No MeSH data available.