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Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery

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ABSTRACT

In this study, we determined the number of peripheral blood circulating tumor cells (CTCs) pre- and post-cryosurgery in patients with colorectal cancer liver metastasis as a reference for understanding the relevance of any changes to the efficacy of cryosurgery. CTC numbers and CTC-related gene expression were measured in the peripheral blood of 55 patients with colorectal liver metastasis at 1 day before and 7 and 30 d after cryoablation using magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS) combined with real-time quantitative PCR (RT-qPCR). The number of CTCs decreased significantly with postoperative time (P < 0.01). Delta cycle threshold values for the CTC-related genes CEA, Ep-CAM, CK18 and CK19 increased significantly after cryoablation. Furthermore, the expression of CEA, Ep-CAM, CK18 and CK19 decreased significantly with time after cryoablation (P < 0.01). RT-qPCR and FACS combined with MACS has significant diagnostic and prognostic value for evaluating the efficacy of cryosurgery in patients with advanced colorectal cancer.

No MeSH data available.


△Ct values for CTC markers at 1 day before and 7 and 30 d after cryoablation therapy (**P < 0.01, n = 55).
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f0003: △Ct values for CTC markers at 1 day before and 7 and 30 d after cryoablation therapy (**P < 0.01, n = 55).

Mentions: Variable patterns of ΔCt value increases were observed. ΔCt for CEA increased from 2.27 ± 5.203 at baseline to 5.9204 ± 4.995 and 9.77 ± 5.551 at 7 and 30 d postoperatively, respectively; ΔCt for Ep-CAM increased from 2.60 ± 5.853 to 6.81 ± 5.816 and 10.56 ± 6.046; ΔCt for CK18 increased from 4.01 ± 6.049 to 8.23 ± 5.53 and 11.57 ± 5.530; and ΔCt for CK19 increased from 2.68 ± 5.506 to 6.09 ± 4.851 and 9.06 ± 6.005. Random analysis of variance (Fig. 3) showed that the △Ct values for the CTC markers were increased significantly at 7 and 30 d after cryoablation (P < 0.01 vs baseline).Figure 3.


Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery
△Ct values for CTC markers at 1 day before and 7 and 30 d after cryoablation therapy (**P < 0.01, n = 55).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036405&req=5

f0003: △Ct values for CTC markers at 1 day before and 7 and 30 d after cryoablation therapy (**P < 0.01, n = 55).
Mentions: Variable patterns of ΔCt value increases were observed. ΔCt for CEA increased from 2.27 ± 5.203 at baseline to 5.9204 ± 4.995 and 9.77 ± 5.551 at 7 and 30 d postoperatively, respectively; ΔCt for Ep-CAM increased from 2.60 ± 5.853 to 6.81 ± 5.816 and 10.56 ± 6.046; ΔCt for CK18 increased from 4.01 ± 6.049 to 8.23 ± 5.53 and 11.57 ± 5.530; and ΔCt for CK19 increased from 2.68 ± 5.506 to 6.09 ± 4.851 and 9.06 ± 6.005. Random analysis of variance (Fig. 3) showed that the △Ct values for the CTC markers were increased significantly at 7 and 30 d after cryoablation (P < 0.01 vs baseline).Figure 3.

View Article: PubMed Central - PubMed

ABSTRACT

In this study, we determined the number of peripheral blood circulating tumor cells (CTCs) pre- and post-cryosurgery in patients with colorectal cancer liver metastasis as a reference for understanding the relevance of any changes to the efficacy of cryosurgery. CTC numbers and CTC-related gene expression were measured in the peripheral blood of 55 patients with colorectal liver metastasis at 1&nbsp;day before and 7 and 30 d after cryoablation using magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS) combined with real-time quantitative PCR (RT-qPCR). The number of CTCs decreased significantly with postoperative time (P &lt; 0.01). Delta cycle threshold values for the CTC-related genes CEA, Ep-CAM, CK18 and CK19 increased significantly after cryoablation. Furthermore, the expression of CEA, Ep-CAM, CK18 and CK19 decreased significantly with time after cryoablation (P &lt; 0.01). RT-qPCR and FACS combined with MACS has significant diagnostic and prognostic value for evaluating the efficacy of cryosurgery in patients with advanced colorectal cancer.

No MeSH data available.