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Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery

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ABSTRACT

In this study, we determined the number of peripheral blood circulating tumor cells (CTCs) pre- and post-cryosurgery in patients with colorectal cancer liver metastasis as a reference for understanding the relevance of any changes to the efficacy of cryosurgery. CTC numbers and CTC-related gene expression were measured in the peripheral blood of 55 patients with colorectal liver metastasis at 1 day before and 7 and 30 d after cryoablation using magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS) combined with real-time quantitative PCR (RT-qPCR). The number of CTCs decreased significantly with postoperative time (P < 0.01). Delta cycle threshold values for the CTC-related genes CEA, Ep-CAM, CK18 and CK19 increased significantly after cryoablation. Furthermore, the expression of CEA, Ep-CAM, CK18 and CK19 decreased significantly with time after cryoablation (P < 0.01). RT-qPCR and FACS combined with MACS has significant diagnostic and prognostic value for evaluating the efficacy of cryosurgery in patients with advanced colorectal cancer.

No MeSH data available.


Flow cytometric enumeration of CTCs at 1 day before and 7 and 30 d after cryoablation therapy (P < 0.01, n = 55).
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f0002: Flow cytometric enumeration of CTCs at 1 day before and 7 and 30 d after cryoablation therapy (P < 0.01, n = 55).

Mentions: Peripheral blood CTCs were analyzed in all 55 patients before and after cryosurgical treatment for CRML (Fig. 2). At 1 day before cryoablation (baseline), the mean number of CTCs was 23.69 ± 9.593; the mean numbers at 7 d and 30 d after the procedure were 19.29 ± 9.506 and 12.62 ± 6.178, respectively. Random analysis of variance (Table 3) showed that CTC numbers were decreased significantly at 7 and 30 d after cryoablation (P < 0.01 vs baseline).Figure 2.


Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery
Flow cytometric enumeration of CTCs at 1 day before and 7 and 30 d after cryoablation therapy (P < 0.01, n = 55).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036405&req=5

f0002: Flow cytometric enumeration of CTCs at 1 day before and 7 and 30 d after cryoablation therapy (P < 0.01, n = 55).
Mentions: Peripheral blood CTCs were analyzed in all 55 patients before and after cryosurgical treatment for CRML (Fig. 2). At 1 day before cryoablation (baseline), the mean number of CTCs was 23.69 ± 9.593; the mean numbers at 7 d and 30 d after the procedure were 19.29 ± 9.506 and 12.62 ± 6.178, respectively. Random analysis of variance (Table 3) showed that CTC numbers were decreased significantly at 7 and 30 d after cryoablation (P < 0.01 vs baseline).Figure 2.

View Article: PubMed Central - PubMed

ABSTRACT

In this study, we determined the number of peripheral blood circulating tumor cells (CTCs) pre- and post-cryosurgery in patients with colorectal cancer liver metastasis as a reference for understanding the relevance of any changes to the efficacy of cryosurgery. CTC numbers and CTC-related gene expression were measured in the peripheral blood of 55 patients with colorectal liver metastasis at 1&nbsp;day before and 7 and 30 d after cryoablation using magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS) combined with real-time quantitative PCR (RT-qPCR). The number of CTCs decreased significantly with postoperative time (P &lt; 0.01). Delta cycle threshold values for the CTC-related genes CEA, Ep-CAM, CK18 and CK19 increased significantly after cryoablation. Furthermore, the expression of CEA, Ep-CAM, CK18 and CK19 decreased significantly with time after cryoablation (P &lt; 0.01). RT-qPCR and FACS combined with MACS has significant diagnostic and prognostic value for evaluating the efficacy of cryosurgery in patients with advanced colorectal cancer.

No MeSH data available.