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Filamentation protects Candida albicans fromamphotericin B-induced programmed cell death via a mechanism involving the yeast metacaspase, MCA1

View Article: PubMed Central - PubMed

ABSTRACT

The budding yeast Candida albicans is one of the mostsignificant fungal pathogens worldwide. It proliferates in two distinct celltypes: blastopores and filaments. Only cells that are able to transform from onecell type into the other are virulent in mouse disease models. Programmed celldeath is a controlled form of cell suicide that occurs when C.albicans cells are exposed to fungicidal drugs like amphotericin Band caspofungin, and to other stressful conditions. We now provide evidence thatsuggests that programmed cell death is cell-type specific in yeast: FilamentousC. albicans cells are more resistant to amphotericin B- andcaspofungin-induced programmed cell death than their blastospore counterparts.Finally, our genetic data suggests that this phenomenon is mediated by aprotective mechanism involving the yeast metacaspase, MCA1.

No MeSH data available.


FIGURE 3: Filamentous C. albicans cells are moreresistant than blastospores to caspofungin-induced programmed celldeath. Representative confocal scanning laser fluorescence images of wild-typeSC5314 C. albicans cells treated with 0.05 μg/ml caspofungin for 3 hours inYPD. Propidium iodide stains dead cells, dihydrorhodamine 123 (DHR123)indicates the presence of reactive oxygen species (ROS), and the FLICA assaystains for cells with activated intracellular caspases. No PI or FLICApositive cells were observed in the no drug controls. Error bars indicatestandard deviations for trials with at least three independent cultures,where at least 300 cells were counted for each trial. A single asteriskindicates statistical significance (p < 0.05) as compared to treatedcontrols. Statistical significance was determined with the unpairedStudent’s t-test.
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Fig3: FIGURE 3: Filamentous C. albicans cells are moreresistant than blastospores to caspofungin-induced programmed celldeath. Representative confocal scanning laser fluorescence images of wild-typeSC5314 C. albicans cells treated with 0.05 μg/ml caspofungin for 3 hours inYPD. Propidium iodide stains dead cells, dihydrorhodamine 123 (DHR123)indicates the presence of reactive oxygen species (ROS), and the FLICA assaystains for cells with activated intracellular caspases. No PI or FLICApositive cells were observed in the no drug controls. Error bars indicatestandard deviations for trials with at least three independent cultures,where at least 300 cells were counted for each trial. A single asteriskindicates statistical significance (p < 0.05) as compared to treatedcontrols. Statistical significance was determined with the unpairedStudent’s t-test.


Filamentation protects Candida albicans fromamphotericin B-induced programmed cell death via a mechanism involving the yeast metacaspase, MCA1
FIGURE 3: Filamentous C. albicans cells are moreresistant than blastospores to caspofungin-induced programmed celldeath. Representative confocal scanning laser fluorescence images of wild-typeSC5314 C. albicans cells treated with 0.05 μg/ml caspofungin for 3 hours inYPD. Propidium iodide stains dead cells, dihydrorhodamine 123 (DHR123)indicates the presence of reactive oxygen species (ROS), and the FLICA assaystains for cells with activated intracellular caspases. No PI or FLICApositive cells were observed in the no drug controls. Error bars indicatestandard deviations for trials with at least three independent cultures,where at least 300 cells were counted for each trial. A single asteriskindicates statistical significance (p < 0.05) as compared to treatedcontrols. Statistical significance was determined with the unpairedStudent’s t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036395&req=5

Fig3: FIGURE 3: Filamentous C. albicans cells are moreresistant than blastospores to caspofungin-induced programmed celldeath. Representative confocal scanning laser fluorescence images of wild-typeSC5314 C. albicans cells treated with 0.05 μg/ml caspofungin for 3 hours inYPD. Propidium iodide stains dead cells, dihydrorhodamine 123 (DHR123)indicates the presence of reactive oxygen species (ROS), and the FLICA assaystains for cells with activated intracellular caspases. No PI or FLICApositive cells were observed in the no drug controls. Error bars indicatestandard deviations for trials with at least three independent cultures,where at least 300 cells were counted for each trial. A single asteriskindicates statistical significance (p < 0.05) as compared to treatedcontrols. Statistical significance was determined with the unpairedStudent’s t-test.

View Article: PubMed Central - PubMed

ABSTRACT

The budding yeast Candida albicans is one of the mostsignificant fungal pathogens worldwide. It proliferates in two distinct celltypes: blastopores and filaments. Only cells that are able to transform from onecell type into the other are virulent in mouse disease models. Programmed celldeath is a controlled form of cell suicide that occurs when C.albicans cells are exposed to fungicidal drugs like amphotericin Band caspofungin, and to other stressful conditions. We now provide evidence thatsuggests that programmed cell death is cell-type specific in yeast: FilamentousC. albicans cells are more resistant to amphotericin B- andcaspofungin-induced programmed cell death than their blastospore counterparts.Finally, our genetic data suggests that this phenomenon is mediated by aprotective mechanism involving the yeast metacaspase, MCA1.

No MeSH data available.