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Active Shiga-Like Toxin Produced by Some Aeromonas spp., Isolated in Mexico City

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ABSTRACT

Shiga-like toxins (Stx) represent a group of bacterial toxins involved in human and animal diseases. Stx is produced by enterohemorrhagic Escherichia coli, Shigella dysenteriae type 1, Citrobacter freundii, and Aeromonas spp.; Stx is an important cause of bloody diarrhea and hemolytic uremic syndrome (HUS). The aim of this study was to identify the stx1/stx2 genes in clinical strains and outer membrane vesicles (OMVs) of Aeromonas spp., 66 strains were isolated from children who live in Mexico City, and Stx effects were evaluated in Vero cell cultures. The capacity to express active Stx1 and Stx2 toxins was determined in Vero cell cultures and the concentration of Stx was evaluated by 50% lethal dose (LD50) assays, observing inhibition of damaged cells by specific monoclonal antibodies. The results obtained in this study support the hypothesis that the stx gene is another putative virulence factor of Aeromonas, and since this gene can be transferred horizontally through OMVs this genus should be included as a possible causal agents of gastroenteritis and it should be reported as part of standard health surveillance procedures. Furthermore, these results indicate that the Aeromonas genus might be a potential causative agent of HUS.

No MeSH data available.


Electropherogram of stx1 gene amplification from OMVs’ DNA. (1) Kapa Universal Ladder, (2) Negative control, (3) OMVs of A. hydrophila F-0050, (4) A. hydrophila F-0050, (5) OMVs of E. coli O157:H7, and (6) E. coli O157:H7.
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Figure 4: Electropherogram of stx1 gene amplification from OMVs’ DNA. (1) Kapa Universal Ladder, (2) Negative control, (3) OMVs of A. hydrophila F-0050, (4) A. hydrophila F-0050, (5) OMVs of E. coli O157:H7, and (6) E. coli O157:H7.

Mentions: Polymerase chain reaction of OMVs’ DNA of A. hydrophila F-0050 revealed the OMVs contained the gen stx1; hence, OMVs might be a potential vehicle for horizontal virulence genes transfer (Figure 4).


Active Shiga-Like Toxin Produced by Some Aeromonas spp., Isolated in Mexico City
Electropherogram of stx1 gene amplification from OMVs’ DNA. (1) Kapa Universal Ladder, (2) Negative control, (3) OMVs of A. hydrophila F-0050, (4) A. hydrophila F-0050, (5) OMVs of E. coli O157:H7, and (6) E. coli O157:H7.
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Related In: Results  -  Collection

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Figure 4: Electropherogram of stx1 gene amplification from OMVs’ DNA. (1) Kapa Universal Ladder, (2) Negative control, (3) OMVs of A. hydrophila F-0050, (4) A. hydrophila F-0050, (5) OMVs of E. coli O157:H7, and (6) E. coli O157:H7.
Mentions: Polymerase chain reaction of OMVs’ DNA of A. hydrophila F-0050 revealed the OMVs contained the gen stx1; hence, OMVs might be a potential vehicle for horizontal virulence genes transfer (Figure 4).

View Article: PubMed Central - PubMed

ABSTRACT

Shiga-like toxins (Stx) represent a group of bacterial toxins involved in human and animal diseases. Stx is produced by enterohemorrhagic Escherichia coli, Shigella dysenteriae type 1, Citrobacter freundii, and Aeromonas spp.; Stx is an important cause of bloody diarrhea and hemolytic uremic syndrome (HUS). The aim of this study was to identify the stx1/stx2 genes in clinical strains and outer membrane vesicles (OMVs) of Aeromonas spp., 66 strains were isolated from children who live in Mexico City, and Stx effects were evaluated in Vero cell cultures. The capacity to express active Stx1 and Stx2 toxins was determined in Vero cell cultures and the concentration of Stx was evaluated by 50% lethal dose (LD50) assays, observing inhibition of damaged cells by specific monoclonal antibodies. The results obtained in this study support the hypothesis that the stx gene is another putative virulence factor of Aeromonas, and since this gene can be transferred horizontally through OMVs this genus should be included as a possible causal agents of gastroenteritis and it should be reported as part of standard health surveillance procedures. Furthermore, these results indicate that the Aeromonas genus might be a potential causative agent of HUS.

No MeSH data available.