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The Clinical Significance of MiR-429 as a Predictive Biomarker in Colorectal Cancer Patients Receiving 5-Fluorouracil Treatment

View Article: PubMed Central - PubMed

ABSTRACT

Background: 5-Fluorouracil (5-FU) based treatment is the standard therapy for metastatic colorectal cancer (CRC), but the development of chemoresistance is inevitable. Increasing evidence shows that dysregulation of microRNAs (miRNAs) is involved in malignant transformation. Thus, it is imperative that we find new diagnostic and prognostic marker for chemotherapy in CRC.

Material/methods: For clinical parameter analysis, 78 CRC tissues and adjacent normal tissues and 45 serum specimens from CRC patients were included in this study. For chemo-response analysis, 116 primary tissues were collected from the patients receiving first-line 5-FU treatment. Quantitative Real-Time PCR (qRT-PCR) was used to detect microRNAs expression.

Results: The expression of miR-429 was significantly increased in both serum and primary tissues from CRC patients, and enhanced miR-429 level was associated with tumor size, lymph node metastasis, and TNM stage. The diagnostic and prognostic values were also confirmed in CRC by using primary tissues. For patients receiving 5-FU-based treatment, miR-429 levels were significantly lower in responding group. The proportions of patients that did not experience response to therapy were higher in primary tumors with high miR-429 expression levels as compared with primary tumors with low miR-429 expression levels. Finally, Kaplan-Meier survival analysis showed that miR-429 is an independent prognostic indicator for chemo-response to 5-FU therapy among CRC patients.

Conclusions: High level of miR-429 expression was correlated with enhanced malignant potential and poor prognosis of CRC patients. Furthermore, miR-429 could affect the chemo-sensitivity of CRC patients to 5-FU therapy and was associated with poor response to 5-FU-based chemotherapy in patients with CRC.

No MeSH data available.


The diagnostic and prognostic value of miR-429 in CRC tissues. ROC curve was drawn to exhibit the diagnostic capacity of miR-429 (A) and CEA (B). (C) miR-429 expression was negatively correlated with CEA in primary CRC tissues. (D) Patients with high expression of miR-429 were associated with shorter overall survival compared with patients with low expression.
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f2-medscimonit-22-3352: The diagnostic and prognostic value of miR-429 in CRC tissues. ROC curve was drawn to exhibit the diagnostic capacity of miR-429 (A) and CEA (B). (C) miR-429 expression was negatively correlated with CEA in primary CRC tissues. (D) Patients with high expression of miR-429 were associated with shorter overall survival compared with patients with low expression.

Mentions: We next sought to validate the diagnostic role of miR-429 of primary CRC tissues by using the receiver operating characteristic (ROC) curve analysis, and compared it with the traditional CRC marker, CEA. As shown in Figure 2A and 2B, the area under the curve (AUC) of miR-429 were 0.775 (95% CI: 0.702–0.838). The diagnostic sensitivity and specificity was 71.790% and 62.820%, respectively. The AUC of miR-429 was higher than CEA, while its AUC was 0.742 (95% CI: 0.666–0.808), and diagnostic sensitivity and specificity were 70.51% and 64.10%, respectively. And there is no statistical significance of AUC between miR-429 and CEA. Besides, the expression of miR-429 was negatively correlated with CEA (Figure 2C). These data showed that miR-429 had a potential diagnostic value for CRC patients.


The Clinical Significance of MiR-429 as a Predictive Biomarker in Colorectal Cancer Patients Receiving 5-Fluorouracil Treatment
The diagnostic and prognostic value of miR-429 in CRC tissues. ROC curve was drawn to exhibit the diagnostic capacity of miR-429 (A) and CEA (B). (C) miR-429 expression was negatively correlated with CEA in primary CRC tissues. (D) Patients with high expression of miR-429 were associated with shorter overall survival compared with patients with low expression.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC5036382&req=5

f2-medscimonit-22-3352: The diagnostic and prognostic value of miR-429 in CRC tissues. ROC curve was drawn to exhibit the diagnostic capacity of miR-429 (A) and CEA (B). (C) miR-429 expression was negatively correlated with CEA in primary CRC tissues. (D) Patients with high expression of miR-429 were associated with shorter overall survival compared with patients with low expression.
Mentions: We next sought to validate the diagnostic role of miR-429 of primary CRC tissues by using the receiver operating characteristic (ROC) curve analysis, and compared it with the traditional CRC marker, CEA. As shown in Figure 2A and 2B, the area under the curve (AUC) of miR-429 were 0.775 (95% CI: 0.702–0.838). The diagnostic sensitivity and specificity was 71.790% and 62.820%, respectively. The AUC of miR-429 was higher than CEA, while its AUC was 0.742 (95% CI: 0.666–0.808), and diagnostic sensitivity and specificity were 70.51% and 64.10%, respectively. And there is no statistical significance of AUC between miR-429 and CEA. Besides, the expression of miR-429 was negatively correlated with CEA (Figure 2C). These data showed that miR-429 had a potential diagnostic value for CRC patients.

View Article: PubMed Central - PubMed

ABSTRACT

Background: 5-Fluorouracil (5-FU) based treatment is the standard therapy for metastatic colorectal cancer (CRC), but the development of chemoresistance is inevitable. Increasing evidence shows that dysregulation of microRNAs (miRNAs) is involved in malignant transformation. Thus, it is imperative that we find new diagnostic and prognostic marker for chemotherapy in CRC.

Material/methods: For clinical parameter analysis, 78 CRC tissues and adjacent normal tissues and 45 serum specimens from CRC patients were included in this study. For chemo-response analysis, 116 primary tissues were collected from the patients receiving first-line 5-FU treatment. Quantitative Real-Time PCR (qRT-PCR) was used to detect microRNAs expression.

Results: The expression of miR-429 was significantly increased in both serum and primary tissues from CRC patients, and enhanced miR-429 level was associated with tumor size, lymph node metastasis, and TNM stage. The diagnostic and prognostic values were also confirmed in CRC by using primary tissues. For patients receiving 5-FU-based treatment, miR-429 levels were significantly lower in responding group. The proportions of patients that did not experience response to therapy were higher in primary tumors with high miR-429 expression levels as compared with primary tumors with low miR-429 expression levels. Finally, Kaplan-Meier survival analysis showed that miR-429 is an independent prognostic indicator for chemo-response to 5-FU therapy among CRC patients.

Conclusions: High level of miR-429 expression was correlated with enhanced malignant potential and poor prognosis of CRC patients. Furthermore, miR-429 could affect the chemo-sensitivity of CRC patients to 5-FU therapy and was associated with poor response to 5-FU-based chemotherapy in patients with CRC.

No MeSH data available.