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The relationship between non-HDL cholesterol and macrophage phenotypes in human adipose tissue

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ABSTRACT

Data from experimental animal models and in vitro studies suggest that both hyperlipoproteinemia and obesity predispose to development of proinflammatory pathways of macrophages within adipose tissue. The aim of this study was to analyze whether non-HDL cholesterol concentration in healthy living kidney donors (LKDs) is related to the number and phenotype of proinflammatory macrophages in visceral and subcutaneous adipose tissue. Adipose tissue samples were collected by cleansing the kidney grafts of LKDs obtained peroperatively. The stromal vascular fractions of these tissues were analyzed by flow cytometry. Proinflammatory macrophages were defined as CD14+ cells coexpressing CD16+ and high-expression CD36 as well (CD14+CD16+CD36+++), while CD16 negativity and CD163 positivity identified alternatively stimulated, anti-inflammatory macrophages. Non-HDL cholesterol concentration positively correlated to proinflammatory macrophages within visceral adipose tissue, with increased strength with more precise phenotype determination. On the contrary, the proportion of alternatively stimulated macrophages correlated negatively with non-HDL cholesterol. The present study suggests a relationship of non-HDL cholesterol concentration to the number and phenotype proportion of macrophages in visceral adipose tissue of healthy humans.

No MeSH data available.


The relations between different macrophage subpopulations and non-HDL cholesterol concentration in subcutaneous adipose tissue. Correlations of macrophage number and proportion of phenotypes with non-HDL cholesterol in the subcutaneous adipose tissue of 47 LKDs (or their subset in E). Consequently, total CD14+ cells (A), CD14+CD16+ cells (B), CD14+CD16+ % (C), CD14+CD16+CD36+++ % (D), CD14+CD16+CD36+++ % (limitation 2–4 mM) (E), and CD14+CD16−CD163+ % (F).
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f3: The relations between different macrophage subpopulations and non-HDL cholesterol concentration in subcutaneous adipose tissue. Correlations of macrophage number and proportion of phenotypes with non-HDL cholesterol in the subcutaneous adipose tissue of 47 LKDs (or their subset in E). Consequently, total CD14+ cells (A), CD14+CD16+ cells (B), CD14+CD16+ % (C), CD14+CD16+CD36+++ % (D), CD14+CD16+CD36+++ % (limitation 2–4 mM) (E), and CD14+CD16−CD163+ % (F).

Mentions: Although the correlation pattern of the total macrophage and CD14+CD16+ macrophage contents (Fig. 3A, B) and the proportion of CD14+CD16+ and normally stimulated macrophages CD14+, CD16+, CD36+++ (Fig. 3C, D) of subcutaneous adipose tissue to non-HDL cholesterol were similar to those of visceral tissue, the significance was much lower (only P < 0.05) and only borderline in all relations. Also, the correlation of alternatively stimulated macrophages in subcutaneous adipose tissue to non-HDL cholesterol exhibited the same pattern as visceral adipose tissue, but with a lower level of significance (Fig. 3F).


The relationship between non-HDL cholesterol and macrophage phenotypes in human adipose tissue
The relations between different macrophage subpopulations and non-HDL cholesterol concentration in subcutaneous adipose tissue. Correlations of macrophage number and proportion of phenotypes with non-HDL cholesterol in the subcutaneous adipose tissue of 47 LKDs (or their subset in E). Consequently, total CD14+ cells (A), CD14+CD16+ cells (B), CD14+CD16+ % (C), CD14+CD16+CD36+++ % (D), CD14+CD16+CD36+++ % (limitation 2–4 mM) (E), and CD14+CD16−CD163+ % (F).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5036370&req=5

f3: The relations between different macrophage subpopulations and non-HDL cholesterol concentration in subcutaneous adipose tissue. Correlations of macrophage number and proportion of phenotypes with non-HDL cholesterol in the subcutaneous adipose tissue of 47 LKDs (or their subset in E). Consequently, total CD14+ cells (A), CD14+CD16+ cells (B), CD14+CD16+ % (C), CD14+CD16+CD36+++ % (D), CD14+CD16+CD36+++ % (limitation 2–4 mM) (E), and CD14+CD16−CD163+ % (F).
Mentions: Although the correlation pattern of the total macrophage and CD14+CD16+ macrophage contents (Fig. 3A, B) and the proportion of CD14+CD16+ and normally stimulated macrophages CD14+, CD16+, CD36+++ (Fig. 3C, D) of subcutaneous adipose tissue to non-HDL cholesterol were similar to those of visceral tissue, the significance was much lower (only P < 0.05) and only borderline in all relations. Also, the correlation of alternatively stimulated macrophages in subcutaneous adipose tissue to non-HDL cholesterol exhibited the same pattern as visceral adipose tissue, but with a lower level of significance (Fig. 3F).

View Article: PubMed Central - PubMed

ABSTRACT

Data from experimental animal models and in vitro studies suggest that both hyperlipoproteinemia and obesity predispose to development of proinflammatory pathways of macrophages within adipose tissue. The aim of this study was to analyze whether non-HDL cholesterol concentration in healthy living kidney donors (LKDs) is related to the number and phenotype of proinflammatory macrophages in visceral and subcutaneous adipose tissue. Adipose tissue samples were collected by cleansing the kidney grafts of LKDs obtained peroperatively. The stromal vascular fractions of these tissues were analyzed by flow cytometry. Proinflammatory macrophages were defined as CD14+ cells coexpressing CD16+ and high-expression CD36 as well (CD14+CD16+CD36+++), while CD16 negativity and CD163 positivity identified alternatively stimulated, anti-inflammatory macrophages. Non-HDL cholesterol concentration positively correlated to proinflammatory macrophages within visceral adipose tissue, with increased strength with more precise phenotype determination. On the contrary, the proportion of alternatively stimulated macrophages correlated negatively with non-HDL cholesterol. The present study suggests a relationship of non-HDL cholesterol concentration to the number and phenotype proportion of macrophages in visceral adipose tissue of healthy humans.

No MeSH data available.