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High-speed microscopy of continuously moving cell culture vessels

View Article: PubMed Central - PubMed

ABSTRACT

We report a method of high-speed phase contrast and bright field microscopy which permits large cell culture vessels to be scanned at much higher speed (up to 30 times faster) than when conventional methods are used without compromising image quality. The object under investigation moves continuously and is captured using a flash illumination which creates an exposure time short enough to prevent motion blur. During the scan the object always stays in focus due to a novel hardware-autofocus system.

No MeSH data available.


Linearity of the peak positions when the object is evenly elevated by the piezo stage from 0 to 300 μm.Note that the peak positions travel in opposite direction. This is because one of the peaks was deliberately mirrored by setting the length of the reference arm accordingly. The advantage of this is that the peak positions can be easily separated and do not extend into each other’s range which facilitates the peak determination. Furthermore the range of the two peaks can be controlled in order not to overlap with the autocorrelation peak visible in Fig. 5.
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f9: Linearity of the peak positions when the object is evenly elevated by the piezo stage from 0 to 300 μm.Note that the peak positions travel in opposite direction. This is because one of the peaks was deliberately mirrored by setting the length of the reference arm accordingly. The advantage of this is that the peak positions can be easily separated and do not extend into each other’s range which facilitates the peak determination. Furthermore the range of the two peaks can be controlled in order not to overlap with the autocorrelation peak visible in Fig. 5.

Mentions: The factor K can be constant as there is a linear relationship between the peak and the geometrical position of the reflection. The value of K corresponds to the inverse slope of the straight line which is generated when plotting the peak positions over the geometrical shift of the object in axial direction preferably by using the calibrated piezo z-stage (Fig. 9).


High-speed microscopy of continuously moving cell culture vessels
Linearity of the peak positions when the object is evenly elevated by the piezo stage from 0 to 300 μm.Note that the peak positions travel in opposite direction. This is because one of the peaks was deliberately mirrored by setting the length of the reference arm accordingly. The advantage of this is that the peak positions can be easily separated and do not extend into each other’s range which facilitates the peak determination. Furthermore the range of the two peaks can be controlled in order not to overlap with the autocorrelation peak visible in Fig. 5.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036042&req=5

f9: Linearity of the peak positions when the object is evenly elevated by the piezo stage from 0 to 300 μm.Note that the peak positions travel in opposite direction. This is because one of the peaks was deliberately mirrored by setting the length of the reference arm accordingly. The advantage of this is that the peak positions can be easily separated and do not extend into each other’s range which facilitates the peak determination. Furthermore the range of the two peaks can be controlled in order not to overlap with the autocorrelation peak visible in Fig. 5.
Mentions: The factor K can be constant as there is a linear relationship between the peak and the geometrical position of the reflection. The value of K corresponds to the inverse slope of the straight line which is generated when plotting the peak positions over the geometrical shift of the object in axial direction preferably by using the calibrated piezo z-stage (Fig. 9).

View Article: PubMed Central - PubMed

ABSTRACT

We report a method of high-speed phase contrast and bright field microscopy which permits large cell culture vessels to be scanned at much higher speed (up to 30 times faster) than when conventional methods are used without compromising image quality. The object under investigation moves continuously and is captured using a flash illumination which creates an exposure time short enough to prevent motion blur. During the scan the object always stays in focus due to a novel hardware-autofocus system.

No MeSH data available.