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High-speed microscopy of continuously moving cell culture vessels

View Article: PubMed Central - PubMed

ABSTRACT

We report a method of high-speed phase contrast and bright field microscopy which permits large cell culture vessels to be scanned at much higher speed (up to 30 times faster) than when conventional methods are used without compromising image quality. The object under investigation moves continuously and is captured using a flash illumination which creates an exposure time short enough to prevent motion blur. During the scan the object always stays in focus due to a novel hardware-autofocus system.

No MeSH data available.


Options for coupling the focus measurement beam into the microscope.(a) Schematic, (b) photo of modified camera side port. Note that the imaging camera is mounted to another camera port of the microscope on the photo.
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f4: Options for coupling the focus measurement beam into the microscope.(a) Schematic, (b) photo of modified camera side port. Note that the imaging camera is mounted to another camera port of the microscope on the photo.

Mentions: It measures the focus position several thousand times per second with no moving parts thereby allowing the object to move uninterruptedly during measurement. A near-infrared laser beam at 840 nm is coupled into the microscope through a beam splitter in front of the C-mount camera port allowing synchronous focus measurements and imaging of the moving object (Fig. 4).


High-speed microscopy of continuously moving cell culture vessels
Options for coupling the focus measurement beam into the microscope.(a) Schematic, (b) photo of modified camera side port. Note that the imaging camera is mounted to another camera port of the microscope on the photo.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036042&req=5

f4: Options for coupling the focus measurement beam into the microscope.(a) Schematic, (b) photo of modified camera side port. Note that the imaging camera is mounted to another camera port of the microscope on the photo.
Mentions: It measures the focus position several thousand times per second with no moving parts thereby allowing the object to move uninterruptedly during measurement. A near-infrared laser beam at 840 nm is coupled into the microscope through a beam splitter in front of the C-mount camera port allowing synchronous focus measurements and imaging of the moving object (Fig. 4).

View Article: PubMed Central - PubMed

ABSTRACT

We report a method of high-speed phase contrast and bright field microscopy which permits large cell culture vessels to be scanned at much higher speed (up to 30 times faster) than when conventional methods are used without compromising image quality. The object under investigation moves continuously and is captured using a flash illumination which creates an exposure time short enough to prevent motion blur. During the scan the object always stays in focus due to a novel hardware-autofocus system.

No MeSH data available.