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High-speed microscopy of continuously moving cell culture vessels

View Article: PubMed Central - PubMed

ABSTRACT

We report a method of high-speed phase contrast and bright field microscopy which permits large cell culture vessels to be scanned at much higher speed (up to 30 times faster) than when conventional methods are used without compromising image quality. The object under investigation moves continuously and is captured using a flash illumination which creates an exposure time short enough to prevent motion blur. During the scan the object always stays in focus due to a novel hardware-autofocus system.

No MeSH data available.


Schematic representation of the focus measurement system based on a fiber-coupled Michelson interferometer design.Light coming from the superluminescent diode (SLD) is split into a reference and a measurement arm and is reflected from a fixed reference mirror and from the various interfaces of the object (microtiter plate - MTP). The reflected light portions are combined in the spectrometer arm where they interfere with each other. The interference pattern is captured by an optical spectrometer.
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f3: Schematic representation of the focus measurement system based on a fiber-coupled Michelson interferometer design.Light coming from the superluminescent diode (SLD) is split into a reference and a measurement arm and is reflected from a fixed reference mirror and from the various interfaces of the object (microtiter plate - MTP). The reflected light portions are combined in the spectrometer arm where they interfere with each other. The interference pattern is captured by an optical spectrometer.

Mentions: For this purpose, we have developed an interferometric focus measurement system based on Fourier domain optical coherence tomography (FD-OCT)13 (Fig. 3).


High-speed microscopy of continuously moving cell culture vessels
Schematic representation of the focus measurement system based on a fiber-coupled Michelson interferometer design.Light coming from the superluminescent diode (SLD) is split into a reference and a measurement arm and is reflected from a fixed reference mirror and from the various interfaces of the object (microtiter plate - MTP). The reflected light portions are combined in the spectrometer arm where they interfere with each other. The interference pattern is captured by an optical spectrometer.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036042&req=5

f3: Schematic representation of the focus measurement system based on a fiber-coupled Michelson interferometer design.Light coming from the superluminescent diode (SLD) is split into a reference and a measurement arm and is reflected from a fixed reference mirror and from the various interfaces of the object (microtiter plate - MTP). The reflected light portions are combined in the spectrometer arm where they interfere with each other. The interference pattern is captured by an optical spectrometer.
Mentions: For this purpose, we have developed an interferometric focus measurement system based on Fourier domain optical coherence tomography (FD-OCT)13 (Fig. 3).

View Article: PubMed Central - PubMed

ABSTRACT

We report a method of high-speed phase contrast and bright field microscopy which permits large cell culture vessels to be scanned at much higher speed (up to 30 times faster) than when conventional methods are used without compromising image quality. The object under investigation moves continuously and is captured using a flash illumination which creates an exposure time short enough to prevent motion blur. During the scan the object always stays in focus due to a novel hardware-autofocus system.

No MeSH data available.