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High-speed microscopy of continuously moving cell culture vessels

View Article: PubMed Central - PubMed

ABSTRACT

We report a method of high-speed phase contrast and bright field microscopy which permits large cell culture vessels to be scanned at much higher speed (up to 30 times faster) than when conventional methods are used without compromising image quality. The object under investigation moves continuously and is captured using a flash illumination which creates an exposure time short enough to prevent motion blur. During the scan the object always stays in focus due to a novel hardware-autofocus system.

No MeSH data available.


Implementation of the high-speed microscopy solution with the OCT-based autofocus system on an inverted Nikon Ti-E microscope.The fibre of the focus measurement system is coupled into the microscope using a modification on the fluorescence port. See also the dotted line in the drawing of Fig. 4a. Coupling via the camera port is shown in Fig. 4b.
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f2: Implementation of the high-speed microscopy solution with the OCT-based autofocus system on an inverted Nikon Ti-E microscope.The fibre of the focus measurement system is coupled into the microscope using a modification on the fluorescence port. See also the dotted line in the drawing of Fig. 4a. Coupling via the camera port is shown in Fig. 4b.

Mentions: We have developed a high-speed microscopy solution using the latest hard- and software technology and a novel autofocus technique that fulfils both requirements (Figs 1 and 2).


High-speed microscopy of continuously moving cell culture vessels
Implementation of the high-speed microscopy solution with the OCT-based autofocus system on an inverted Nikon Ti-E microscope.The fibre of the focus measurement system is coupled into the microscope using a modification on the fluorescence port. See also the dotted line in the drawing of Fig. 4a. Coupling via the camera port is shown in Fig. 4b.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036042&req=5

f2: Implementation of the high-speed microscopy solution with the OCT-based autofocus system on an inverted Nikon Ti-E microscope.The fibre of the focus measurement system is coupled into the microscope using a modification on the fluorescence port. See also the dotted line in the drawing of Fig. 4a. Coupling via the camera port is shown in Fig. 4b.
Mentions: We have developed a high-speed microscopy solution using the latest hard- and software technology and a novel autofocus technique that fulfils both requirements (Figs 1 and 2).

View Article: PubMed Central - PubMed

ABSTRACT

We report a method of high-speed phase contrast and bright field microscopy which permits large cell culture vessels to be scanned at much higher speed (up to 30 times faster) than when conventional methods are used without compromising image quality. The object under investigation moves continuously and is captured using a flash illumination which creates an exposure time short enough to prevent motion blur. During the scan the object always stays in focus due to a novel hardware-autofocus system.

No MeSH data available.