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High-speed microscopy of continuously moving cell culture vessels

View Article: PubMed Central - PubMed

ABSTRACT

We report a method of high-speed phase contrast and bright field microscopy which permits large cell culture vessels to be scanned at much higher speed (up to 30 times faster) than when conventional methods are used without compromising image quality. The object under investigation moves continuously and is captured using a flash illumination which creates an exposure time short enough to prevent motion blur. During the scan the object always stays in focus due to a novel hardware-autofocus system.

No MeSH data available.


Benefit of shading correction in rendering the single tiles almost invisible.(a) Without shading correction, (b) with shading correction.
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f12: Benefit of shading correction in rendering the single tiles almost invisible.(a) Without shading correction, (b) with shading correction.

Mentions: Apart from software-based image stitching to enhance the accuracy of the alignment we apply a shading correction algorithm to compensate for illumination inhomogeneities on each image tile24. This improves the overall appearance of the image as it renders the single tiles almost invisible as can be seen when comparing a stitched image of one well with and without shading correction (Fig. 12).


High-speed microscopy of continuously moving cell culture vessels
Benefit of shading correction in rendering the single tiles almost invisible.(a) Without shading correction, (b) with shading correction.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036042&req=5

f12: Benefit of shading correction in rendering the single tiles almost invisible.(a) Without shading correction, (b) with shading correction.
Mentions: Apart from software-based image stitching to enhance the accuracy of the alignment we apply a shading correction algorithm to compensate for illumination inhomogeneities on each image tile24. This improves the overall appearance of the image as it renders the single tiles almost invisible as can be seen when comparing a stitched image of one well with and without shading correction (Fig. 12).

View Article: PubMed Central - PubMed

ABSTRACT

We report a method of high-speed phase contrast and bright field microscopy which permits large cell culture vessels to be scanned at much higher speed (up to 30 times faster) than when conventional methods are used without compromising image quality. The object under investigation moves continuously and is captured using a flash illumination which creates an exposure time short enough to prevent motion blur. During the scan the object always stays in focus due to a novel hardware-autofocus system.

No MeSH data available.