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High-speed microscopy of continuously moving cell culture vessels

View Article: PubMed Central - PubMed

ABSTRACT

We report a method of high-speed phase contrast and bright field microscopy which permits large cell culture vessels to be scanned at much higher speed (up to 30 times faster) than when conventional methods are used without compromising image quality. The object under investigation moves continuously and is captured using a flash illumination which creates an exposure time short enough to prevent motion blur. During the scan the object always stays in focus due to a novel hardware-autofocus system.

No MeSH data available.


Feature detection using SURF keypoint descriptors in the overlapping image region.(a) Translation between images of (40, −4) pixels. (b) Alignment of both images after compensation of detected translation.
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f10: Feature detection using SURF keypoint descriptors in the overlapping image region.(a) Translation between images of (40, −4) pixels. (b) Alignment of both images after compensation of detected translation.

Mentions: The response time of the motion control and trigger generation based on the stage positions, is 40 μs with the motion controller used. This allows the fields of view on the object to be captured quite precisely but not with perfectly equidistant spacing. The latency of the trigger signal leads to stochastic variations in the position of the field of view up to 40 μs times the scan velocity. To correct for this, we implemented a fast stitching procedure. Neighbouring fields are captured with a slight overlap and the relative position to each other is detected using GPU accelerated feature detection based on the SURF (speeded up robust features) algorithm21 (Fig. 10).


High-speed microscopy of continuously moving cell culture vessels
Feature detection using SURF keypoint descriptors in the overlapping image region.(a) Translation between images of (40, −4) pixels. (b) Alignment of both images after compensation of detected translation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036042&req=5

f10: Feature detection using SURF keypoint descriptors in the overlapping image region.(a) Translation between images of (40, −4) pixels. (b) Alignment of both images after compensation of detected translation.
Mentions: The response time of the motion control and trigger generation based on the stage positions, is 40 μs with the motion controller used. This allows the fields of view on the object to be captured quite precisely but not with perfectly equidistant spacing. The latency of the trigger signal leads to stochastic variations in the position of the field of view up to 40 μs times the scan velocity. To correct for this, we implemented a fast stitching procedure. Neighbouring fields are captured with a slight overlap and the relative position to each other is detected using GPU accelerated feature detection based on the SURF (speeded up robust features) algorithm21 (Fig. 10).

View Article: PubMed Central - PubMed

ABSTRACT

We report a method of high-speed phase contrast and bright field microscopy which permits large cell culture vessels to be scanned at much higher speed (up to 30 times faster) than when conventional methods are used without compromising image quality. The object under investigation moves continuously and is captured using a flash illumination which creates an exposure time short enough to prevent motion blur. During the scan the object always stays in focus due to a novel hardware-autofocus system.

No MeSH data available.