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High-speed microscopy of continuously moving cell culture vessels

View Article: PubMed Central - PubMed

ABSTRACT

We report a method of high-speed phase contrast and bright field microscopy which permits large cell culture vessels to be scanned at much higher speed (up to 30 times faster) than when conventional methods are used without compromising image quality. The object under investigation moves continuously and is captured using a flash illumination which creates an exposure time short enough to prevent motion blur. During the scan the object always stays in focus due to a novel hardware-autofocus system.

No MeSH data available.


Special hardware components necessary to upgrade a conventional microscope to a high-speed scanning system featuring image acquisition of moving objects (permission to publish the product photos is kindly granted by the manufacturers Märzhäuser, Gardasoft and PCO).
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f1: Special hardware components necessary to upgrade a conventional microscope to a high-speed scanning system featuring image acquisition of moving objects (permission to publish the product photos is kindly granted by the manufacturers Märzhäuser, Gardasoft and PCO).

Mentions: We have developed a high-speed microscopy solution using the latest hard- and software technology and a novel autofocus technique that fulfils both requirements (Figs 1 and 2).


High-speed microscopy of continuously moving cell culture vessels
Special hardware components necessary to upgrade a conventional microscope to a high-speed scanning system featuring image acquisition of moving objects (permission to publish the product photos is kindly granted by the manufacturers Märzhäuser, Gardasoft and PCO).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036042&req=5

f1: Special hardware components necessary to upgrade a conventional microscope to a high-speed scanning system featuring image acquisition of moving objects (permission to publish the product photos is kindly granted by the manufacturers Märzhäuser, Gardasoft and PCO).
Mentions: We have developed a high-speed microscopy solution using the latest hard- and software technology and a novel autofocus technique that fulfils both requirements (Figs 1 and 2).

View Article: PubMed Central - PubMed

ABSTRACT

We report a method of high-speed phase contrast and bright field microscopy which permits large cell culture vessels to be scanned at much higher speed (up to 30 times faster) than when conventional methods are used without compromising image quality. The object under investigation moves continuously and is captured using a flash illumination which creates an exposure time short enough to prevent motion blur. During the scan the object always stays in focus due to a novel hardware-autofocus system.

No MeSH data available.