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MALDI-TOF-MS reveals differential N -linked plasma- and IgG-glycosylation profiles between mothers and their newborns

View Article: PubMed Central - PubMed

ABSTRACT

During pregnancy, the mother provides multiple nutrients and substances to the foetus, with maternal immunoglobulin G (IgG) being actively transported to the foetus. Newborns depend on maternal IgG for immune-protection in their first months. The glycosylation of IgG has been shown to influence its dynamics, e.g. receptor binding. While minor differences in IgG glycosylation have been found between IgG derived from maternal blood and umbilical cord blood (UC) of newborn children, the differential glycosylation of maternal and UC plasma has hitherto not been studied. Here, we studied the N-glycosylation of IgG and total plasma proteome of both maternal and UC plasma of 42 pairs of mothers and newborn children. A total of 37 N-glycans were quantified for IgG and 45 for the total plasma N-glycome (TPNG). The study showed slightly higher levels of galactosylation for UC IgG than maternal IgG, confirming previous results, as well as lower bisection and sialylation. Furthermore, the TPNG results showed lower values for galactosylation and sialylation, and higher values for fucosylation in the UC plasma. In conclusion, this study presents some novel insights into IgG glycosylation differences as well as the first broad overview of the differential plasma glycosylation between mothers and newborns.

No MeSH data available.


Glycosylation differences between maternal and UC TPNG.The differences in TPNG glycosylation are presented for each individual pair as well as the entire group. Six relevant derived traits are presented, namely (A) % fucosylation, (B) % sialylation per antenna, (C) ratio of α2,3-linked/α2,6-linked sialylation per galactose of complex compositions, (D) % galactosylation per antenna, (E) % sialylation per galactose and (F) % glycan compositions unique to IgG, being the non sialylated fucosylated diantennary glycans.
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f4: Glycosylation differences between maternal and UC TPNG.The differences in TPNG glycosylation are presented for each individual pair as well as the entire group. Six relevant derived traits are presented, namely (A) % fucosylation, (B) % sialylation per antenna, (C) ratio of α2,3-linked/α2,6-linked sialylation per galactose of complex compositions, (D) % galactosylation per antenna, (E) % sialylation per galactose and (F) % glycan compositions unique to IgG, being the non sialylated fucosylated diantennary glycans.

Mentions: UC plasma showed higher levels of hybrid glycans (Hy; mother versus UC: 0.6% vs. 0.9%; p = 5.4∙10−4), diantennary glycans (A2; 77.1% vs. 85.1%; p = 1.0∙10−5), fucosylation (F; 31.1% vs. 39.7%; p = 6.0∙10−6; Fig. 4A) and glycan compositions supposedly unique to IgG (IGG; 11.9% vs. 20.1%; p = 1.1∙10−5; Fig. 4F). Glycan trait levels of UC were lower for galactosylation per antenna (AG; 94.8% vs. 92.9%; p = 1.1∙10−4; Fig. 4D), sialylation per antenna (AS; 75.8% vs. 65.5%; p = 1.3∙10−5; Fig. 4B), α2,3-linked sialylation per antenna (AL; 12.0% vs. 7.9%; p = 9.4∙10−6), α2,6-linked sialylation per antenna (AE; 63.8% vs. 57.6%; p = 5.0∙10−5), sialylation per galactose (GS; 79.7% vs. 70.4%; p = 1.3∙10−5; Fig. 4E), α2,3-linked sialylation per galactose (GL; 12.4% vs. 8.4%; p = 9.4∙10−6), α2,6-linked sialylation per galactose (GE; 67.3% vs. 62.0%; p = 6.3∙10−5) and the percentage of sialylation on complex compositions being α2,3-linked (AGRle; 15.6% vs. 11.8%; p = 1.1∙10−5; Fig. 4C). A summary of all derived traits is presented in Table 2.


MALDI-TOF-MS reveals differential N -linked plasma- and IgG-glycosylation profiles between mothers and their newborns
Glycosylation differences between maternal and UC TPNG.The differences in TPNG glycosylation are presented for each individual pair as well as the entire group. Six relevant derived traits are presented, namely (A) % fucosylation, (B) % sialylation per antenna, (C) ratio of α2,3-linked/α2,6-linked sialylation per galactose of complex compositions, (D) % galactosylation per antenna, (E) % sialylation per galactose and (F) % glycan compositions unique to IgG, being the non sialylated fucosylated diantennary glycans.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5036037&req=5

f4: Glycosylation differences between maternal and UC TPNG.The differences in TPNG glycosylation are presented for each individual pair as well as the entire group. Six relevant derived traits are presented, namely (A) % fucosylation, (B) % sialylation per antenna, (C) ratio of α2,3-linked/α2,6-linked sialylation per galactose of complex compositions, (D) % galactosylation per antenna, (E) % sialylation per galactose and (F) % glycan compositions unique to IgG, being the non sialylated fucosylated diantennary glycans.
Mentions: UC plasma showed higher levels of hybrid glycans (Hy; mother versus UC: 0.6% vs. 0.9%; p = 5.4∙10−4), diantennary glycans (A2; 77.1% vs. 85.1%; p = 1.0∙10−5), fucosylation (F; 31.1% vs. 39.7%; p = 6.0∙10−6; Fig. 4A) and glycan compositions supposedly unique to IgG (IGG; 11.9% vs. 20.1%; p = 1.1∙10−5; Fig. 4F). Glycan trait levels of UC were lower for galactosylation per antenna (AG; 94.8% vs. 92.9%; p = 1.1∙10−4; Fig. 4D), sialylation per antenna (AS; 75.8% vs. 65.5%; p = 1.3∙10−5; Fig. 4B), α2,3-linked sialylation per antenna (AL; 12.0% vs. 7.9%; p = 9.4∙10−6), α2,6-linked sialylation per antenna (AE; 63.8% vs. 57.6%; p = 5.0∙10−5), sialylation per galactose (GS; 79.7% vs. 70.4%; p = 1.3∙10−5; Fig. 4E), α2,3-linked sialylation per galactose (GL; 12.4% vs. 8.4%; p = 9.4∙10−6), α2,6-linked sialylation per galactose (GE; 67.3% vs. 62.0%; p = 6.3∙10−5) and the percentage of sialylation on complex compositions being α2,3-linked (AGRle; 15.6% vs. 11.8%; p = 1.1∙10−5; Fig. 4C). A summary of all derived traits is presented in Table 2.

View Article: PubMed Central - PubMed

ABSTRACT

During pregnancy, the mother provides multiple nutrients and substances to the foetus, with maternal immunoglobulin G (IgG) being actively transported to the foetus. Newborns depend on maternal IgG for immune-protection in their first months. The glycosylation of IgG has been shown to influence its dynamics, e.g. receptor binding. While minor differences in IgG glycosylation have been found between IgG derived from maternal blood and umbilical cord blood (UC) of newborn children, the differential glycosylation of maternal and UC plasma has hitherto not been studied. Here, we studied the N-glycosylation of IgG and total plasma proteome of both maternal and UC plasma of 42 pairs of mothers and newborn children. A total of 37 N-glycans were quantified for IgG and 45 for the total plasma N-glycome (TPNG). The study showed slightly higher levels of galactosylation for UC IgG than maternal IgG, confirming previous results, as well as lower bisection and sialylation. Furthermore, the TPNG results showed lower values for galactosylation and sialylation, and higher values for fucosylation in the UC plasma. In conclusion, this study presents some novel insights into IgG glycosylation differences as well as the first broad overview of the differential plasma glycosylation between mothers and newborns.

No MeSH data available.