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Compartmentalized gene expression profiling of receptive endometrium reveals progesterone regulated ENPP3 is differentially expressed and secreted in glycosylated form

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ABSTRACT

The complexity of endometrial receptivity at the molecular level needs to be explored in detail to improve the management of infertility. Here, differential expression of transcriptomes in receptive endometrial glands and stroma revealed Ectonucleotide Pyrophosphatase/Phosphodiesterase 3 (ENPP3) as a progesterone regulated factor and confirmed by various methods, both at mRNA and protein level. The involvement of ENPP3 in embryo attachment was tested in an in vitro model for human embryo implantation. Interestingly, there was high expression of ENPP3 mRNA in stroma but not protein. Presence of N-glycosylated ENPP3 in receptive phase uterine fluid in women confirms its regulation by progesterone and makes it possible to use in a non-invasive test of endometrial receptivity.

No MeSH data available.


Expression of ENPP3 in the uterine fluid of receptive and non-receptive phase.Tukey plot for the expression of ENPP3 quantified by nano-ESI-LC/MS in uterine fluids from early secretory phase (LH + 2) and mid-secretory phase (LH + 8) of the same set of women. A significant upregulation of ENPP3 (p = 0.0032) was observed in P dominant, receptive phase (LH + 8) on comparison with non-receptive phase (LH + 2).
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f8: Expression of ENPP3 in the uterine fluid of receptive and non-receptive phase.Tukey plot for the expression of ENPP3 quantified by nano-ESI-LC/MS in uterine fluids from early secretory phase (LH + 2) and mid-secretory phase (LH + 8) of the same set of women. A significant upregulation of ENPP3 (p = 0.0032) was observed in P dominant, receptive phase (LH + 8) on comparison with non-receptive phase (LH + 2).

Mentions: We identified and quantified the expression of ENPP3 protein with 35 ENPP3 specific peptides obtaining 53.4% sequence coverage in the mass-spectrometry of uterine fluid. Based on total normalized protein intensities, ENPP3 protein was observed to be upregulated (mean fold change + 35.0, p = 0.003) during P dominant LH + 8, on comparing with early secretory phase (LH + 2) of the natural menstrual cycle (Fig. 8).


Compartmentalized gene expression profiling of receptive endometrium reveals progesterone regulated ENPP3 is differentially expressed and secreted in glycosylated form
Expression of ENPP3 in the uterine fluid of receptive and non-receptive phase.Tukey plot for the expression of ENPP3 quantified by nano-ESI-LC/MS in uterine fluids from early secretory phase (LH + 2) and mid-secretory phase (LH + 8) of the same set of women. A significant upregulation of ENPP3 (p = 0.0032) was observed in P dominant, receptive phase (LH + 8) on comparison with non-receptive phase (LH + 2).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036034&req=5

f8: Expression of ENPP3 in the uterine fluid of receptive and non-receptive phase.Tukey plot for the expression of ENPP3 quantified by nano-ESI-LC/MS in uterine fluids from early secretory phase (LH + 2) and mid-secretory phase (LH + 8) of the same set of women. A significant upregulation of ENPP3 (p = 0.0032) was observed in P dominant, receptive phase (LH + 8) on comparison with non-receptive phase (LH + 2).
Mentions: We identified and quantified the expression of ENPP3 protein with 35 ENPP3 specific peptides obtaining 53.4% sequence coverage in the mass-spectrometry of uterine fluid. Based on total normalized protein intensities, ENPP3 protein was observed to be upregulated (mean fold change + 35.0, p = 0.003) during P dominant LH + 8, on comparing with early secretory phase (LH + 2) of the natural menstrual cycle (Fig. 8).

View Article: PubMed Central - PubMed

ABSTRACT

The complexity of endometrial receptivity at the molecular level needs to be explored in detail to improve the management of infertility. Here, differential expression of transcriptomes in receptive endometrial glands and stroma revealed Ectonucleotide Pyrophosphatase/Phosphodiesterase 3 (ENPP3) as a progesterone regulated factor and confirmed by various methods, both at mRNA and protein level. The involvement of ENPP3 in embryo attachment was tested in an in vitro model for human embryo implantation. Interestingly, there was high expression of ENPP3 mRNA in stroma but not protein. Presence of N-glycosylated ENPP3 in receptive phase uterine fluid in women confirms its regulation by progesterone and makes it possible to use in a non-invasive test of endometrial receptivity.

No MeSH data available.